##gff-version 3
##sequence-region BBa_K301007 1 3032
BBa_K301007	.	engineered_region	96	3032	.	+	0	ID=BBa_K301010;Name=BBa_K301010
BBa_K301007	.	promoter	2141	2163	.	+	0	ID=BBa_K301003;Name=BBa_K301003;Parent=BBa_K301010
BBa_K301007	.	promoter	2141	2163	.	+	0	ID=annotation2099978;Name=promoter;Parent=BBa_K301003
BBa_K301007	.	promoter	1104	1126	.	+	0	ID=BBa_K301003;Name=BBa_K301003;Parent=BBa_K301010
BBa_K301007	.	promoter	1104	1126	.	+	0	ID=annotation2099978;Name=promoter;Parent=BBa_K301003
BBa_K301007	.	CDS	96	1095	.	+	0	ID=BBa_K301005;Name=BBa_K301005;Parent=BBa_K301010
BBa_K301007	.	CDS	1133	2132	.	+	0	ID=BBa_K301005;Name=BBa_K301005;Parent=BBa_K301010
BBa_K301007	.	engineered_region	2172	3032	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_K301010
BBa_K301007	.	CDS	2190	2870	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K301007	.	CDS	2190	2864	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K301007	.	engineered_tag	2871	2895	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K301007	.	terminator	2992	3032	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K301007	.	engineered_region	2992	3032	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K301007	.	stem_loop	2999	3018	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K301007	.	stop_codon	3025	3025	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K301007	.	polyA_site	3019	3032	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K301007	.	ribosome_entry_site	2172	2183	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K301007	.	conserved_region	2176	2179	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K301007	.	terminator	2904	2983	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K301007	.	engineered_region	2904	2983	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K301007	.	stem_loop	2915	2958	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K301007	.	promoter	1	89	.	+	0	ID=BBa_K239006;Name=BBa_K239006
BBa_K301007	.	non_covalent_binding_site	42	55	.	+	0	ID=annotation2006722;Name=Fnr;Parent=BBa_K239006
BBa_K301007	.	non_covalent_binding_site	38	56	.	+	0	ID=annotation2006723;Name=Fis;Parent=BBa_K239006
BBa_K301007	.	non_covalent_binding_site	4	17	.	+	0	ID=annotation2006724;Name=Fnr;Parent=BBa_K239006
BBa_K301007	.	promoter	76	83	.	+	0	ID=annotation2006720;Name=-10;Parent=BBa_K239006
BBa_K301007	.	non_covalent_binding_site	20	26	.	+	0	ID=annotation2006725;Name=NarL;Parent=BBa_K239006
BBa_K301007	.	promoter	48	56	.	+	0	ID=annotation2006721;Name=-35;Parent=BBa_K239006
BBa_K301007	.	non_covalent_binding_site	7	13	.	+	0	ID=annotation2006726;Name=NarL;Parent=BBa_K239006
>BBa_K301007
gtattgataaatatcaatgatagataaagttatcttatcgtttgatttacatcaaattgcctttagctacagacactaa
ggtggcagactactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttc
aacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtg
aagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcat
cactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccg
acagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaa
ccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcg
tatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaag
aaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcata
aggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgcca
aaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgca
ggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtg
gctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgt
ttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtactagagtaa
tacgactcactatagggagatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgc
tatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttac
gagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgcca
agcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcgg
caagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctg
gcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctc
gcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggca
cgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtct
tcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagct
tacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgc
aacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcatt
actggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgct
acgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagt
actagagtaatacgactcactatagggagatactagagaaagaggagaaatactagatggcttcctccgaagacgttat
caaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaa
ggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcc
tgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcctt
cccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctg
caagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaa
ccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaa
actgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgct
tacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaag
gtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata