BBa_K301007
type: ComponentDefinition
persistent identity: BBa K301007
display id: BBa_K301007
version: 1
was generated by: iGEM to SBOL conversion
description: Low Oxygen Level Self-induction Genetic Circuit. mNarK promoter
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: ..
owned by: james
owned by: myers
mutable description: Similar to the device "Low Oxygen Level Self-induction Genetic Circuit. NarK promoter". mNarK promoter is designed to have better affinity to activated Fnr than the NarK promoter (BBa_K239005) and hence also stronger activity under anaerobic conditions. It is also shorter than the NarK promoter (BBa_K239005).
discontinued: false
dominant: false
group u list: _484_
m user id: 0
owner id: 5136
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: ..
star: false
creator: Xiang Chen
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K301010
component: BBa_K239006
sequence annotation: BBa_K239006
sequence annotation: BBa_K301010
sequence: https://synbiohub.org/public/igem/BBa_K301007_sequence/1

BBa_K301010
type: Component
persistent identity: component2107043
display id: component2107043
version: 1
top level: BBa_K301007
definition: BBa_K301010
access: public

BBa_K239006
type: Component
persistent identity: component2107024
display id: component2107024
version: 1
top level: BBa_K301007
definition: BBa_K239006
access: public

BBa_K239006
type: SequenceAnnotation
persistent identity: annotation2107024
display id: annotation2107024
version: 1
top level: BBa_K301007
location: https://synbiohub.org/public/igem/BBa_K301007/annotation2107024/range2107024/1
component: BBa_K239006

https://synbiohub.org/public/igem/BBa_K301007/annotation2107024/range2107024/1
type: Range
persistent identity: range2107024
display id: range2107024
version: 1
top level: BBa_K301007
direction: direction/reverse
start: 1
end: 89
orientation: inline

BBa_K301010
type: SequenceAnnotation
persistent identity: annotation2107043
display id: annotation2107043
version: 1
top level: BBa_K301007
location: https://synbiohub.org/public/igem/BBa_K301007/annotation2107043/range2107043/1
component: BBa_K301010

https://synbiohub.org/public/igem/BBa_K301007/annotation2107043/range2107043/1
type: Range
persistent identity: range2107043
display id: range2107043
version: 1
top level: BBa_K301007
direction: direction/reverse
start: 96
end: 3032
orientation: inline

BBa_K301010
type: ComponentDefinition
persistent identity: BBa K301010
display id: BBa_K301010
version: 1
was generated by: iGEM to SBOL conversion
description: Self-induction Genetic Circuit
created: 2010-10-26T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: ..
owned by: james
owned by: myers
mutable description: This composite part contains the self-inducing positive loop and a downstream reporter, RFP. Can be jointed with different promoters which can response to various conditions as a complete self-induction system as required.
discontinued: false
dominant: false
group u list: _484_
m user id: 0
owner id: 5136
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: ..
star: false
creator: Xiang Chen
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K301003
component: BBa_K301005
component: BBa_I13507
sequence annotation: BBa_K301005
sequence annotation: BBa_K301003
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_K301010_sequence/1

BBa_K301003
type: Component
persistent identity: component2106960
display id: component2106960
version: 1
top level: BBa_K301010
definition: BBa_K301003
access: public

BBa_K301005
type: Component
persistent identity: component2106958
display id: component2106958
version: 1
top level: BBa_K301010
definition: BBa_K301005
access: public

BBa_K301003
type: Component
persistent identity: component2106957
display id: component2106957
version: 1
top level: BBa_K301010
definition: BBa_K301003
access: public

BBa_K301005
type: Component
persistent identity: component2106955
display id: component2106955
version: 1
top level: BBa_K301010
definition: BBa_K301005
access: public

BBa_I13507
type: Component
persistent identity: component2106972
display id: component2106972
version: 1
top level: BBa_K301010
definition: BBa_I13507
access: public

BBa_K301005
type: SequenceAnnotation
persistent identity: annotation2106955
display id: annotation2106955
version: 1
top level: BBa_K301010
location: https://synbiohub.org/public/igem/BBa_K301010/annotation2106955/range2106955/1
component: BBa_K301005

https://synbiohub.org/public/igem/BBa_K301010/annotation2106955/range2106955/1
type: Range
persistent identity: range2106955
display id: range2106955
version: 1
top level: BBa_K301010
direction: direction/reverse
start: 1
end: 1000
orientation: inline

BBa_K301003
type: SequenceAnnotation
persistent identity: annotation2106957
display id: annotation2106957
version: 1
top level: BBa_K301010
location: https://synbiohub.org/public/igem/BBa_K301010/annotation2106957/range2106957/1
component: BBa_K301003

https://synbiohub.org/public/igem/BBa_K301010/annotation2106957/range2106957/1
type: Range
persistent identity: range2106957
display id: range2106957
version: 1
top level: BBa_K301010
direction: direction/reverse
start: 1009
end: 1031
orientation: inline

BBa_K301005
type: SequenceAnnotation
persistent identity: annotation2106958
display id: annotation2106958
version: 1
top level: BBa_K301010
location: https://synbiohub.org/public/igem/BBa_K301010/annotation2106958/range2106958/1
component: BBa_K301005

https://synbiohub.org/public/igem/BBa_K301010/annotation2106958/range2106958/1
type: Range
persistent identity: range2106958
display id: range2106958
version: 1
top level: BBa_K301010
direction: direction/reverse
start: 1038
end: 2037
orientation: inline

BBa_K301003
type: SequenceAnnotation
persistent identity: annotation2106960
display id: annotation2106960
version: 1
top level: BBa_K301010
location: https://synbiohub.org/public/igem/BBa_K301010/annotation2106960/range2106960/1
component: BBa_K301003

https://synbiohub.org/public/igem/BBa_K301010/annotation2106960/range2106960/1
type: Range
persistent identity: range2106960
display id: range2106960
version: 1
top level: BBa_K301010
direction: direction/reverse
start: 2046
end: 2068
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2106972
display id: annotation2106972
version: 1
top level: BBa_K301010
location: https://synbiohub.org/public/igem/BBa_K301010/annotation2106972/range2106972/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_K301010/annotation2106972/range2106972/1
type: Range
persistent identity: range2106972
display id: range2106972
version: 1
top level: BBa_K301010
direction: direction/reverse
start: 2077
end: 2937
orientation: inline

BBa_K239006
type: ComponentDefinition
persistent identity: BBa K239006
display id: BBa_K239006
version: 1
was generated by: iGEM to SBOL conversion
description: Modified NarK promoter
created: 2009-06-25T11:00:00Z
modified: 2015-05-08T01:11:36Z
mutable provenance: Raw genomic sequence from biocyc, Escherichia coli K-12, but modified.
owned by: james
owned by: myers
mutable description: Sequence contains 2 Fnr (one of which is modified), 1 Fis, 2 NarL binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions. Area of application: Detection of when the cell is switching to anaerobic metabolism.
discontinued: false
dominant: false
group u list: _375_
m user id: 0
owner id: 4247
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first FNR binding site and ends 6 pb after the -10 box. One pb has been changed in order optimise the first FNR binding site.
star: false
creator: Axel Nystrom
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Fnr
sequence annotation: NarL
sequence annotation: Fis
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K239006_sequence/1

Fnr
type: SequenceAnnotation
persistent identity: annotation2006724
display id: annotation2006724
version: 1
top level: BBa_K239006
location: https://synbiohub.org/public/igem/BBa_K239006/annotation2006724/range2006724/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K239006/annotation2006724/range2006724/1
type: Range
persistent identity: range2006724
display id: range2006724
version: 1
top level: BBa_K239006
direction: direction/forward
start: 4
end: 17
orientation: inline

NarL
type: SequenceAnnotation
persistent identity: annotation2006726
display id: annotation2006726
version: 1
top level: BBa_K239006
location: https://synbiohub.org/public/igem/BBa_K239006/annotation2006726/range2006726/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K239006/annotation2006726/range2006726/1
type: Range
persistent identity: range2006726
display id: range2006726
version: 1
top level: BBa_K239006
direction: direction/forward
start: 7
end: 13
orientation: inline

NarL
type: SequenceAnnotation
persistent identity: annotation2006725
display id: annotation2006725
version: 1
top level: BBa_K239006
location: https://synbiohub.org/public/igem/BBa_K239006/annotation2006725/range2006725/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K239006/annotation2006725/range2006725/1
type: Range
persistent identity: range2006725
display id: range2006725
version: 1
top level: BBa_K239006
direction: direction/forward
start: 20
end: 26
orientation: inline

Fis
type: SequenceAnnotation
persistent identity: annotation2006723
display id: annotation2006723
version: 1
top level: BBa_K239006
location: https://synbiohub.org/public/igem/BBa_K239006/annotation2006723/range2006723/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K239006/annotation2006723/range2006723/1
type: Range
persistent identity: range2006723
display id: range2006723
version: 1
top level: BBa_K239006
direction: direction/forward
start: 38
end: 56
orientation: inline

Fnr
type: SequenceAnnotation
persistent identity: annotation2006722
display id: annotation2006722
version: 1
top level: BBa_K239006
location: https://synbiohub.org/public/igem/BBa_K239006/annotation2006722/range2006722/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K239006/annotation2006722/range2006722/1
type: Range
persistent identity: range2006722
display id: range2006722
version: 1
top level: BBa_K239006
direction: direction/forward
start: 42
end: 55
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2006721
display id: annotation2006721
version: 1
top level: BBa_K239006
location: https://synbiohub.org/public/igem/BBa_K239006/annotation2006721/range2006721/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K239006/annotation2006721/range2006721/1
type: Range
persistent identity: range2006721
display id: range2006721
version: 1
top level: BBa_K239006
direction: direction/forward
start: 48
end: 56
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2006720
display id: annotation2006720
version: 1
top level: BBa_K239006
location: https://synbiohub.org/public/igem/BBa_K239006/annotation2006720/range2006720/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K239006/annotation2006720/range2006720/1
type: Range
persistent identity: range2006720
display id: range2006720
version: 1
top level: BBa_K239006
direction: direction/forward
start: 76
end: 83
orientation: inline

BBa_I13507
type: ComponentDefinition
persistent identity: BBa I13507
display id: BBa_I13507
version: 1
was generated by: iGEM to SBOL conversion
description: Screening plasmid intermediate
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Built by Josh as an intermediate in screening plasmid construction.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_E1010
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13507_sequence/1

BBa_B0034
type: Component
persistent identity: component1524815
display id: component1524815
version: 1
top level: BBa_I13507
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component1524828
display id: component1524828
version: 1
top level: BBa_I13507
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component1524822
display id: component1524822
version: 1
top level: BBa_I13507
definition: mRFP1
access: public

BBa_B0012
type: Component
persistent identity: component1524838
display id: component1524838
version: 1
top level: BBa_I13507
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1524815
display id: annotation1524815
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
type: Range
persistent identity: range1524815
display id: range1524815
version: 1
top level: BBa_I13507
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1524822
display id: annotation1524822
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
type: Range
persistent identity: range1524822
display id: range1524822
version: 1
top level: BBa_I13507
direction: direction/forward
start: 19
end: 699
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1524828
display id: annotation1524828
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
type: Range
persistent identity: range1524828
display id: range1524828
version: 1
top level: BBa_I13507
direction: direction/forward
start: 733
end: 812
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1524838
display id: annotation1524838
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
type: Range
persistent identity: range1524838
display id: range1524838
version: 1
top level: BBa_I13507
direction: direction/forward
start: 821
end: 861
orientation: inline

BBa_K301003
type: ComponentDefinition
persistent identity: BBa K301003
display id: BBa_K301003
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: T7 promoter sequence
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T7 promoter is used as part of the "positive loop" for the self-inducing system in HYPOXON project. The constitutive promoter regulate the expression of the promoter activator, T7 RNA Polymerase, in order to produce the downstream product continuously.
discontinued: false
dominant: false
group u list: _484_
m user id: 0
owner id: 5136
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Part was obtained by oligonucleotide synthesis.
star: false
creator: Xiang Chen
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K301003_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2099978
display id: annotation2099978
version: 1
top level: BBa_K301003
location: https://synbiohub.org/public/igem/BBa_K301003/annotation2099978/range2099978/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K301003/annotation2099978/range2099978/1
type: Range
persistent identity: range2099978
display id: range2099978
version: 1
top level: BBa_K301003
direction: direction/forward
start: 1
end: 23
orientation: inline

BBa_K301005
type: ComponentDefinition
persistent identity: BBa K301005
display id: BBa_K301005
version: 1
was generated by: iGEM to SBOL conversion
description: T7 RNA Polymerase
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: T7 genome
owned by: james
owned by: myers
mutable description: T7 RNA polymerase from the T7 genome. Used as promoter activator in our HYPOXON genetic circuit.
discontinued: false
dominant: false
experience: experience/None
group u list: _484_
m user id: 0
owner id: 5136
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Adding prefix&suffix sequence in front of and after the original T7 RNAP sequence by PCR is a bit difficult.
star: false
creator: Xiang Chen
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K301005_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

https://synbiohub.org/public/igem/BBa_K301007_sequence/1
type: Sequence
persistent identity: BBa K301007 sequence
display id: BBa_K301007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtattgataaatatcaatgatagataaagttatcttatcgtttgatttacatcaaattgcctttagctacagacactaaggtggcagactactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtactagagtaatacgactcactatagggagatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K301010_sequence/1
type: Sequence
persistent identity: BBa K301010 sequence
display id: BBa_K301010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtactagagtaatacgactcactatagggagatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K239006_sequence/1
type: Sequence
persistent identity: BBa K239006 sequence
display id: BBa_K239006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtattgataaatatcaatgatagataaagttatcttatcgtttgatttacatcaaattgcctttagctacagacactaaggtggcagac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13507_sequence/1
type: Sequence
persistent identity: BBa I13507 sequence
display id: BBa_I13507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K301003_sequence/1
type: Sequence
persistent identity: BBa K301003 sequence
display id: BBa_K301003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K301005_sequence/1
type: Sequence
persistent identity: BBa K301005 sequence
display id: BBa_K301005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z