LOCUS       BBa_K301008             3016 bp    DNA     linear       22-Nov-2024
DEFINITION  Self-induction Genetic Circuit with an Inducible Promoter
ACCESSION   BBa_K301008
VERSION     BBa_K301008.1  
FEATURES             Location/Qualifiers
     promoter        1..73
                     /label=BBa_K301000
     CDS             80..1079
                     /label=BBa_K301005
     promoter        1088..1110
                     /label=promoter
     promoter        1088..1110
                     /label=BBa_K301003
     CDS             1117..2116
                     /label=BBa_K301005
     promoter        2125..2147
                     /label=promoter
     promoter        2125..2147
                     /label=BBa_K301003
     misc_feature    2160..2163
                     /label=conserved
     RBS             2156..2167
                     /label=BBa_B0034
     CDS             2174..2848
                     /label=mrfp1
     misc_feature    2855..2879
                     /label=Help:Barcodes
     CDS             2174..2854
                     /label=BBa_E1010
     stem_loop       2899..2942
                     /label=stem_loop
     misc_feature    2888..2967
                     /label=BBa_B0010
     terminator      2888..2967
                     /label=BBa_B0010
     stem_loop       2983..3002
                     /label=T7 TE
     misc_feature    3009..3009
                     /label=stop
     polyA_site      3003..3016
                     /label=polya
     misc_feature    2976..3016
                     /label=BBa_B0012
     terminator      2976..3016
                     /label=BBa_B0012
     misc_feature    2156..3016
                     /label=BBa_I13507
     misc_feature    80..3016
                     /label=BBa_K301010
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttcacacagg aaatactaga tgaacacgat taacatcgct aagaacgact tctctgacat
      121 cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg
      181 cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat
      241 gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat
      301 cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc
      361 taagcgcggc aagcgcccga cagccttcca gttcctgcaa gaaatcaagc cggaagccgt
      421 agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca atacaaccgt
      481 tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat
      541 ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca acaagcgcgt
      601 agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg
      661 tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt
      721 acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc
      781 tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat
      841 cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc
      901 tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct
      961 ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg tttacatgcc
     1021 tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt
     1081 actagagtaa tacgactcac tatagggaga tactagatga acacgattaa catcgctaag
     1141 aacgacttct ctgacatcga actggctgct atcccgttca acactctggc tgaccattac
     1201 ggtgagcgtt tagctcgcga acagttggcc cttgagcatg agtcttacga gatgggtgaa
     1261 gcacgcttcc gcaagatgtt tgagcgtcaa cttaaagctg gtgaggttgc ggataacgct
     1321 gccgccaagc ctctcatcac taccctactc cctaagatga ttgcacgcat caacgactgg
     1381 tttgaggaag tgaaagctaa gcgcggcaag cgcccgacag ccttccagtt cctgcaagaa
     1441 atcaagccgg aagccgtagc gtacatcacc attaagacca ctctggcttg cctaaccagt
     1501 gctgacaata caaccgttca ggctgtagca agcgcaatcg gtcgggccat tgaggacgag
     1561 gctcgcttcg gtcgtatccg tgaccttgaa gctaagcact tcaagaaaaa cgttgaggaa
     1621 caactcaaca agcgcgtagg gcacgtctac aagaaagcat ttatgcaagt tgtcgaggct
     1681 gacatgctct ctaagggtct actcggtggc gaggcgtggt cttcgtggca taaggaagac
     1741 tctattcatg taggagtacg ctgcatcgag atgctcattg agtcaaccgg aatggttagc
     1801 ttacaccgcc aaaatgctgg cgtagtaggt caagactctg agactatcga actcgcacct
     1861 gaatacgctg aggctatcgc aacccgtgca ggtgcgctgg ctggcatctc tccgatgttc
     1921 caaccttgcg tagttcctcc taagccgtgg actggcatta ctggtggtgg ctattgggct
     1981 aacggtcgtc gtcctctggc gctggtgcgt actcacagta agaaagcact gatgcgctac
     2041 gaagacgttt acatgcctga ggtgtacaaa gcgattaaca ttgcgcaaaa caccgcatgg
     2101 aaaatcaaca agaaagtact agagtaatac gactcactat agggagatac tagagaaaga
     2161 ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt
     2221 tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg
     2281 tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt
     2341 cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc
     2401 ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt
     2461 tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg
     2521 tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat
     2581 gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc
     2641 tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga
     2701 agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga
     2761 catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg
     2821 tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct
     2881 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2941 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     3001 cctttctgcg tttata
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