Self-induction Genetic Circuit with an Inducible Promoter
An inducible promoter, pTAC is added in front of the self-induction circuit as a positive control for the other hypoxia detecting devices. Tac promoter (BBa_K301000)is functional properly, as tested with an RFP reporter.
This composite part contains the self-inducing positive loop and a downstream reporter, RFP. Can be jointed with different promoters which can response to various conditions as a complete self-induction system as required.
Tac promoter, hybrid derived from trp and lac promoters
Tac promoter, hybrid derived from trp and lac promoters/.//////
The part is over 100 bp with prefix and suffix, therefore the oligos were designed as small fragments and then join together.
Built by Josh as an intermediate in screening plasmid construction.
T7 promoter is used as part of the "positive loop" for the self-inducing system in HYPOXON project. The constitutive promoter regulate the expression of the promoter activator, T7 RNA Polymerase, in order to produce the downstream product continuously.
Part was obtained by oligonucleotide synthesis.
T7 RNA polymerase from the T7 genome. Used as promoter activator in our HYPOXON genetic circuit.
Adding prefix&suffix sequence in front of and after the original T7 RNAP sequence by PCR is a bit difficult.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Campbell et al., PNAS v99 p7877 URL
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
Conversion of the iGEM parts registry to SBOL2.1