LOCUS       BBa_K301010             2937 bp    DNA     linear       22-Nov-2024
DEFINITION  Self-induction Genetic Circuit
ACCESSION   BBa_K301010
VERSION     BBa_K301010.1  
FEATURES             Location/Qualifiers
     CDS             1..1000
                     /label=BBa_K301005
     promoter        1009..1031
                     /label=promoter
     promoter        1009..1031
                     /label=BBa_K301003
     CDS             1038..2037
                     /label=BBa_K301005
     promoter        2046..2068
                     /label=promoter
     promoter        2046..2068
                     /label=BBa_K301003
     misc_feature    2081..2084
                     /label=conserved
     RBS             2077..2088
                     /label=BBa_B0034
     CDS             2095..2769
                     /label=mrfp1
     misc_feature    2776..2800
                     /label=Help:Barcodes
     CDS             2095..2775
                     /label=BBa_E1010
     stem_loop       2820..2863
                     /label=stem_loop
     misc_feature    2809..2888
                     /label=BBa_B0010
     terminator      2809..2888
                     /label=BBa_B0010
     stem_loop       2904..2923
                     /label=T7 TE
     misc_feature    2930..2930
                     /label=stop
     polyA_site      2924..2937
                     /label=polya
     misc_feature    2897..2937
                     /label=BBa_B0012
     terminator      2897..2937
                     /label=BBa_B0012
     misc_feature    2077..2937
                     /label=BBa_I13507
ORIGIN
        1 atgaacacga ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg
       61 ttcaacactc tggctgacca ttacggtgag cgtttagctc gcgaacagtt ggcccttgag
      121 catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa
      181 gctggtgagg ttgcggataa cgctgccgcc aagcctctca tcactaccct actccctaag
      241 atgattgcac gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg caagcgcccg
      301 acagccttcc agttcctgca agaaatcaag ccggaagccg tagcgtacat caccattaag
      361 accactctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt agcaagcgca
      421 atcggtcggg ccattgagga cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag
      481 cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt ctacaagaaa
      541 gcatttatgc aagttgtcga ggctgacatg ctctctaagg gtctactcgg tggcgaggcg
      601 tggtcttcgt ggcataagga agactctatt catgtaggag tacgctgcat cgagatgctc
      661 attgagtcaa ccggaatggt tagcttacac cgccaaaatg ctggcgtagt aggtcaagac
      721 tctgagacta tcgaactcgc acctgaatac gctgaggcta tcgcaacccg tgcaggtgcg
      781 ctggctggca tctctccgat gttccaacct tgcgtagttc ctcctaagcc gtggactggc
      841 attactggtg gtggctattg ggctaacggt cgtcgtcctc tggcgctggt gcgtactcac
      901 agtaagaaag cactgatgcg ctacgaagac gtttacatgc ctgaggtgta caaagcgatt
      961 aacattgcgc aaaacaccgc atggaaaatc aacaagaaag tactagagta atacgactca
     1021 ctatagggag atactagatg aacacgatta acatcgctaa gaacgacttc tctgacatcg
     1081 aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt ttagctcgcg
     1141 aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc cgcaagatgt
     1201 ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag cctctcatca
     1261 ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa gtgaaagcta
     1321 agcgcggcaa gcgcccgaca gccttccagt tcctgcaaga aatcaagccg gaagccgtag
     1381 cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat acaaccgttc
     1441 aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc
     1501 gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac aagcgcgtag
     1561 ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc
     1621 tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat gtaggagtac
     1681 gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc caaaatgctg
     1741 gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct gaggctatcg
     1801 caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc gtagttcctc
     1861 ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt cgtcctctgg
     1921 cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg
     1981 aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac aagaaagtac
     2041 tagagtaata cgactcacta tagggagata ctagagaaag aggagaaata ctagatggct
     2101 tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt
     2161 aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag
     2221 accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc
     2281 ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac
     2341 ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt
     2401 ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt
     2461 aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt
     2521 tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa
     2581 atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg
     2641 gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc
     2701 tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc
     2761 accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa
     2821 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2881 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//