LOCUS       BBa_K302000              930 bp    DNA     linear       22-Dec-2024
DEFINITION  rocF RBS and coding sequence
ACCESSION   BBa_K302000
VERSION     BBa_K302000.1  
FEATURES             Location/Qualifiers
     misc_feature    27..29
                     /label=start
     misc_feature    915..919
                     /label=Double stop
     misc_feature    227..227
                     /label=Single base mutation to remove EcoR1 site
     misc_feature    824..824
                     /label=	Single base mutation to remove EcoR1 site
ORIGIN
        1 tcttaaatgt tgaggtggaa tcacagatgg ataaaacgat ttcggttatt ggaatgccaa
       61 tggatttagg acaagcacga cgcggagtgg atatgggccc gagtgccatc cggtacgctc
      121 atctgatcga gaggctgtca gacatggggt atacggttga agatctcggt gacattccga
      181 tcaatcgcga aaaaatcaaa aatgacgagg aactgaaaaa cctgaactcc gttttggcgg
      241 gaaatgaaaa actcgcgcaa aaggtcaaca aagtcattga agagaaaaaa ttcccgcttg
      301 tcctgggcgg tgaccacagt attgcgatcg gcacgcttgc aggcacagcg aagcattacg
      361 ataatctcgg cgtcatctgg tatgacgcgc acggcgattt gaatacactt gaaacttcac
      421 catcgggcaa tattcacggc atgccgctcg cggtcagcct aggcattggc cacgagtcac
      481 tggttaacct tgaaggctac gcgcctaaaa tcaaaccgga aaacgtcgtc atcattggcg
      541 cccggtcact tgatgaaggg gagcgcaagt acattaagga aagcggcatg aaggtgtaca
      601 caatgcacga aatcgatcgt cttggcatga caaaggtcat tgaagaaacc cttgattatt
      661 tatcagcatg tgatggcgtc catctgagcc ttgatctgga cggacttgat ccgaacgacg
      721 caccgggtgt cggaacccct gtcgtcggcg gcatcagcta ccgggagagc catttggcta
      781 tggaaatgct gtatgacgca ggcatcatta cctcagccga atttgttgag gttaacccga
      841 tccttgatca caaaaacaaa acgggcaaaa cagcagtaga gctcgtagaa tccctgttag
      901 ggaagaagct gctgtaataa gaaaaccccc
//