##gff-version 3
##sequence-region BBa_K302000 27 919
BBa_K302000	.	sequence_alteration	227	227	.	+	0	ID=annotation2077770;Name=Single base mutation to remove EcoR1 site
BBa_K302000	.	stop_codon	915	919	.	+	0	ID=annotation2077660;Name=Double stop
BBa_K302000	.	start_codon	27	29	.	+	0	ID=annotation2077659;Name=start
BBa_K302000	.	sequence_alteration	824	824	.	+	0	ID=annotation2077771;Name=	Single base mutation to remove EcoR1 site
>BBa_K302000
tcttaaatgttgaggtggaatcacagatggataaaacgatttcggttattggaatgccaatggatttaggacaagcacg
acgcggagtggatatgggcccgagtgccatccggtacgctcatctgatcgagaggctgtcagacatggggtatacggtt
gaagatctcggtgacattccgatcaatcgcgaaaaaatcaaaaatgacgaggaactgaaaaacctgaactccgttttgg
cgggaaatgaaaaactcgcgcaaaaggtcaacaaagtcattgaagagaaaaaattcccgcttgtcctgggcggtgacca
cagtattgcgatcggcacgcttgcaggcacagcgaagcattacgataatctcggcgtcatctggtatgacgcgcacggc
gatttgaatacacttgaaacttcaccatcgggcaatattcacggcatgccgctcgcggtcagcctaggcattggccacg
agtcactggttaaccttgaaggctacgcgcctaaaatcaaaccggaaaacgtcgtcatcattggcgcccggtcacttga
tgaaggggagcgcaagtacattaaggaaagcggcatgaaggtgtacacaatgcacgaaatcgatcgtcttggcatgaca
aaggtcattgaagaaacccttgattatttatcagcatgtgatggcgtccatctgagccttgatctggacggacttgatc
cgaacgacgcaccgggtgtcggaacccctgtcgtcggcggcatcagctaccgggagagccatttggctatggaaatgct
gtatgacgcaggcatcattacctcagccgaatttgttgaggttaacccgatccttgatcacaaaaacaaaacgggcaaa
acagcagtagagctcgtagaatccctgttagggaagaagctgctgtaataagaaaaccccc