BBa_K302012
1
BBa_K302012
IPTG-inducible filamentous cell formation
2010-08-11T11:00:00Z
2015-05-08T01:11:52Z
Genomic DNA from ''Bacillus subtilis'' -''yneA''
Hyperspankoid from oid and hyperspank
''Bacillus subtilis'' in response to stress such as DNA damage stops the cells from dividing. This is a part of the SOS response initiated by the accumulation of single stranded DNA from DNA damage or stalled replication. Two proteins are vital for this response: RecA and LexA. RecA forms filaments on ssDNA and promotes the autocleavage of LexA. LexA usually represses the SOS operon. ''dinR'' is homologous to lexA in ''E. coli'' and is transcribed in the opposite direction of ''yneA''.
''yneA'' stops the formation of ''ftsZ'' ring indirectly. When ''ftsZ'' forms a 30 subunit ring at the midpoint of the cell, it will contract and cause cell division. By expressing ''yneA'' and inhibiting ''ftsZ'' ring formation, the cells will grow filamentous.
By inhibiting cell division, ''yneA'' allows the DNA damage genes to repair the DNA damage before continuing with the cell division cycle. It is hypothesized that ''yneA'' acts through an unknown transmembrane protein to inhibit ''ftsZ'' ring formation; we call this/these unknown components ???Blackbox proteins???. As the evidence shows expression of ''yneA'' leads to filamentation.
false
false
_442_
0
6014
9
It's complicated
true
none
false
Rachel May Boyd, Phil Hall
component2090495
1
BBa_K302003
component2090497
1
BBa_K302005
annotation2090497
1
BBa_K302005
range2090497
1
107
460
annotation2090495
1
BBa_K302003
range2090495
1
1
106
BBa_K302003
1
BBa_K302003
P_hyperspankoid
2010-08-11T11:00:00Z
2015-05-08T01:11:52Z
Combining oid sequence (palindromic) with hyperspank.
Promoter Hyperspankoid for ''B.subtilis'' adapted pspac-oid and hyperspank
false
false
_442_
0
6014
9
Not in stock
true
We used pspacoid and the spacing of the repeat from hyperspank.
false
Rachel May Boyd, Phil Hall and Jem Stach
annotation2091500
1
oid - Lac operator
range2091500
1
81
100
annotation2091499
1
oid - Lac operator
range2091499
1
11
30
annotation2091501
1
pspac promoter
range2091501
1
43
80
BBa_K302005
1
BBa_K302005
yneA RBS and coding sequence
2010-08-11T11:00:00Z
2015-05-08T01:11:52Z
Genomic ''Bacillus subtilis''
''Bacillus subtilis'' in response to stress such as DNA damage stops the cells from dividing. This is a part of the SOS response initiated by the accumulation of single stranded DNA from DNA damage or stalled replication. Two proteins are vital for this response: RecA and LexA. RecA forms filaments on ssDNA and promotes the autocleavage of LexA. LexA usually represses the SOS operon. ''dinR'' is homologous to ''lexA'' in E. coli and is transcribed in the opposite direction of yneA.
yneA stops the formation of ''ftsZ'' ring indirectly. When ''ftsZ'' forms a 30 subunit ring at the midpoint of the cell, it will contract and cause cell division. By expressing ''yneA'' and inhibiting ''ftsZ'' ring formation, the cells will grow filamentous.
By inhibiting cell division, yneA allows the DNA damage genes to repair the DNA damage before continuing with the cell division cycle. It is hypothesized that yneA acts through an unknown transmembrane protein to inhibit ''ftsZ'' ring formation; we call this/these unknown components ???Blackbox proteins???. As the evidence shows expression of ''yneA'' leads to filamentation.
false
false
_442_
0
6014
9
Not in stock
false
None
false
Rachel May Boyd, Phil Hall, Deena Tsu, Jannetta Steyn
annotation2080756
1
Double stop codon
range2080756
1
349
354
BBa_K302012_sequence
1
agaacaacctaattgtgagcgctcacaattttttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcgctcacaattaagcttaagccttactagagaaagaggaggtgaactactatgatcatgagtaaagaatctattatttttgtcggtctgtttacagtgattttgagcgcggttatccttatgctgtcatatacaagcagcggccaagagcttaatcagtatgttaaaatagaagtccagcaaggcgacacactctggtcaattgctgatcaggtagccgatacaaaaaagataaacaaaaatgattttattgaatgggtagctgataaaaatcaacttcaaacctctgatatccagccgggtgatgagttagtgatcccattgaaaaagaagcatcaggatgcatatgaattagcaactgtaagataataa
BBa_K302003_sequence
1
agaacaacctaattgtgagcgctcacaattttttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcgctcacaattaagctt
BBa_K302005_sequence
1
aagccttactagagaaagaggaggtgaactactatgatcatgagtaaagaatctattatttttgtcggtctgtttacagtgattttgagcgcggttatccttatgctgtcatatacaagcagcggccaagagcttaatcagtatgttaaaatagaagtccagcaaggcgacacactctggtcaattgctgatcaggtagccgatacaaaaaagataaacaaaaatgattttattgaatgggtagctgataaaaatcaacttcaaacctctgatatccagccgggtgatgagttagtgatcccattgaaaaagaagcatcaggatgcatatgaattagcaactgtaagataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z