##gff-version 3
##sequence-region BBa_K302014 1 1131
BBa_K302014	.	engineered_region	107	1036	.	+	0	ID=BBa_K302000;Name=BBa_K302000
BBa_K302014	.	sequence_alteration	333	333	.	+	0	ID=annotation2077770;Name=Single base mutation to remove EcoR1 site;Parent=BBa_K302000
BBa_K302014	.	stop_codon	1021	1025	.	+	0	ID=annotation2077660;Name=Double stop;Parent=BBa_K302000
BBa_K302014	.	start_codon	133	135	.	+	0	ID=annotation2077659;Name=start;Parent=BBa_K302000
BBa_K302014	.	sequence_alteration	930	930	.	+	0	ID=annotation2077771;Name=	Single base mutation to remove EcoR1 site;Parent=BBa_K302000
BBa_K302014	.	terminator	1037	1131	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K302014	.	terminator	1037	1077	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K302014	.	stem_loop	1044	1063	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K302014	.	engineered_region	1037	1077	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K302014	.	stop_codon	1070	1070	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K302014	.	polyA_site	1064	1077	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K302014	.	terminator	1086	1131	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K302014	.	stem_loop	1098	1120	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K302014	.	engineered_region	1086	1131	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K302014	.	promoter	1	106	.	+	0	ID=BBa_K174004;Name=BBa_K174004
BBa_K302014	.	operator	81	100	.	+	0	ID=annotation2033093;Name=oid - Lac operator;Parent=BBa_K174004
BBa_K302014	.	promoter	43	80	.	+	0	ID=annotation2033094;Name=pspac promoter;Parent=BBa_K174004
>BBa_K302014
agaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtg
gaattgtgagcgctcacaattaagctttcttaaatgttgaggtggaatcacagatggataaaacgatttcggttattgg
aatgccaatggatttaggacaagcacgacgcggagtggatatgggcccgagtgccatccggtacgctcatctgatcgag
aggctgtcagacatggggtatacggttgaagatctcggtgacattccgatcaatcgcgaaaaaatcaaaaatgacgagg
aactgaaaaacctgaactccgttttggcgggaaatgaaaaactcgcgcaaaaggtcaacaaagtcattgaagagaaaaa
attcccgcttgtcctgggcggtgaccacagtattgcgatcggcacgcttgcaggcacagcgaagcattacgataatctc
ggcgtcatctggtatgacgcgcacggcgatttgaatacacttgaaacttcaccatcgggcaatattcacggcatgccgc
tcgcggtcagcctaggcattggccacgagtcactggttaaccttgaaggctacgcgcctaaaatcaaaccggaaaacgt
cgtcatcattggcgcccggtcacttgatgaaggggagcgcaagtacattaaggaaagcggcatgaaggtgtacacaatg
cacgaaatcgatcgtcttggcatgacaaaggtcattgaagaaacccttgattatttatcagcatgtgatggcgtccatc
tgagccttgatctggacggacttgatccgaacgacgcaccgggtgtcggaacccctgtcgtcggcggcatcagctaccg
ggagagccatttggctatggaaatgctgtatgacgcaggcatcattacctcagccgaatttgttgaggttaacccgatc
cttgatcacaaaaacaaaacgggcaaaacagcagtagagctcgtagaatccctgttagggaagaagctgctgtaataag
aaaaccccctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagat
tattaatccggcttttttattattt