LOCUS       BBa_K302015             1538 bp    DNA     linear       23-Dec-2024
DEFINITION  IPTG-inducible urea/urease
ACCESSION   BBa_K302015
VERSION     BBa_K302015.1  
FEATURES             Location/Qualifiers
     misc_feature    81..100
                     /label=oid - Lac operator
     promoter        43..80
                     /label=pspac promoter
     promoter        1..106
                     /label=BBa_K174004
     misc_feature    121..125
                     /label=predicted pairing
     misc_feature    216..222
                     /label=predicted pairing
     misc_feature    225..233
                     /label=predicted pairing
     misc_feature    236..241
                     /label=predicted pairing
     misc_feature    252..257
                     /label=predicted pairing
     misc_feature    259..264
                     /label=predicted pairing
     misc_feature    282..287
                     /label=predicted pairing
     misc_feature    107..312
                     /label=BBa_K302002
     stem_loop       320..339
                     /label=T7 TE
     misc_feature    346..346
                     /label=stop
     polyA_site      340..353
                     /label=polya
     misc_feature    313..353
                     /label=BBa_B0012
     terminator      313..353
                     /label=BBa_B0012
     stem_loop       374..396
                     /label=stem_loop
     misc_feature    362..407
                     /label=BBa_B0011
     terminator      362..407
                     /label=BBa_B0011
     terminator      313..407
                     /label=BBa_B0014
     misc_feature    1..407
                     /label=BBa_K302013
     misc_feature    488..507
                     /label=oid - Lac operator
     promoter        450..487
                     /label=pspac promoter
     promoter        408..513
                     /label=BBa_K174004
     misc_feature    540..542
                     /label=start
     misc_feature    1428..1432
                     /label=Double stop
     misc_feature    740..740
                     /label=Single base mutation to remove EcoR1 site
     misc_feature    1337..1337
                     /label=	Single base mutation to remove EcoR1 site
     misc_feature    514..1443
                     /label=BBa_K302000
     stem_loop       1451..1470
                     /label=T7 TE
     misc_feature    1477..1477
                     /label=stop
     polyA_site      1471..1484
                     /label=polya
     misc_feature    1444..1484
                     /label=BBa_B0012
     terminator      1444..1484
                     /label=BBa_B0012
     stem_loop       1505..1527
                     /label=stem_loop
     misc_feature    1493..1538
                     /label=BBa_B0011
     terminator      1493..1538
                     /label=BBa_B0011
     terminator      1444..1538
                     /label=BBa_B0014
     misc_feature    408..1538
                     /label=BBa_K302014
ORIGIN
        1 agaacaacct ctgctaaaat tcctgaaaaa ttttgcaaaa agttgttgac tttatctaca
       61 aggtgtggca taatgtgtgg aattgtgagc gctcacaatt aagcttaaca aaagaaatta
      121 agcgttttca aatttcaaag aaatggggaa taagagatgg gtacgattgt ttgccaagat
      181 tgcaacgaag ccattcatta ctttgaagat gagaaagtga caacattata tggaacttgc
      241 tgcggacaat gtcaatgtcc tgttgatgaa gagtaaaaaa aagcatgcgg cttaaagccg
      301 catgcttttt attcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
      361 gagagaatat aaaaagccag attattaatc cggctttttt attatttaga acaacctctg
      421 ctaaaattcc tgaaaaattt tgcaaaaagt tgttgacttt atctacaagg tgtggcataa
      481 tgtgtggaat tgtgagcgct cacaattaag ctttcttaaa tgttgaggtg gaatcacaga
      541 tggataaaac gatttcggtt attggaatgc caatggattt aggacaagca cgacgcggag
      601 tggatatggg cccgagtgcc atccggtacg ctcatctgat cgagaggctg tcagacatgg
      661 ggtatacggt tgaagatctc ggtgacattc cgatcaatcg cgaaaaaatc aaaaatgacg
      721 aggaactgaa aaacctgaac tccgttttgg cgggaaatga aaaactcgcg caaaaggtca
      781 acaaagtcat tgaagagaaa aaattcccgc ttgtcctggg cggtgaccac agtattgcga
      841 tcggcacgct tgcaggcaca gcgaagcatt acgataatct cggcgtcatc tggtatgacg
      901 cgcacggcga tttgaataca cttgaaactt caccatcggg caatattcac ggcatgccgc
      961 tcgcggtcag cctaggcatt ggccacgagt cactggttaa ccttgaaggc tacgcgccta
     1021 aaatcaaacc ggaaaacgtc gtcatcattg gcgcccggtc acttgatgaa ggggagcgca
     1081 agtacattaa ggaaagcggc atgaaggtgt acacaatgca cgaaatcgat cgtcttggca
     1141 tgacaaaggt cattgaagaa acccttgatt atttatcagc atgtgatggc gtccatctga
     1201 gccttgatct ggacggactt gatccgaacg acgcaccggg tgtcggaacc cctgtcgtcg
     1261 gcggcatcag ctaccgggag agccatttgg ctatggaaat gctgtatgac gcaggcatca
     1321 ttacctcagc cgaatttgtt gaggttaacc cgatccttga tcacaaaaac aaaacgggca
     1381 aaacagcagt agagctcgta gaatccctgt tagggaagaa gctgctgtaa taagaaaacc
     1441 ccctcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata
     1501 taaaaagcca gattattaat ccggcttttt tattattt
//