##gff-version 3
##sequence-region BBa_K302015 1 1538
BBa_K302015	.	engineered_region	408	1538	.	+	0	ID=BBa_K302014;Name=BBa_K302014
BBa_K302015	.	promoter	408	513	.	+	0	ID=BBa_K174004;Name=BBa_K174004;Parent=BBa_K302014
BBa_K302015	.	operator	488	507	.	+	0	ID=annotation2033093;Name=oid - Lac operator;Parent=BBa_K174004
BBa_K302015	.	promoter	450	487	.	+	0	ID=annotation2033094;Name=pspac promoter;Parent=BBa_K174004
BBa_K302015	.	terminator	1444	1538	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K302014
BBa_K302015	.	terminator	1444	1484	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K302015	.	polyA_site	1471	1484	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K302015	.	engineered_region	1444	1484	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K302015	.	stem_loop	1451	1470	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K302015	.	stop_codon	1477	1477	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K302015	.	terminator	1493	1538	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K302015	.	stem_loop	1505	1527	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K302015	.	engineered_region	1493	1538	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K302015	.	engineered_region	514	1443	.	+	0	ID=BBa_K302000;Name=BBa_K302000;Parent=BBa_K302014
BBa_K302015	.	sequence_alteration	740	740	.	+	0	ID=annotation2077770;Name=Single base mutation to remove EcoR1 site;Parent=BBa_K302000
BBa_K302015	.	sequence_alteration	1337	1337	.	+	0	ID=annotation2077771;Name=	Single base mutation to remove EcoR1 site;Parent=BBa_K302000
BBa_K302015	.	start_codon	540	542	.	+	0	ID=annotation2077659;Name=start;Parent=BBa_K302000
BBa_K302015	.	stop_codon	1428	1432	.	+	0	ID=annotation2077660;Name=Double stop;Parent=BBa_K302000
BBa_K302015	.	engineered_region	1	407	.	+	0	ID=BBa_K302013;Name=BBa_K302013
BBa_K302015	.	mature_transcript_region	107	312	.	+	0	ID=BBa_K302002;Name=BBa_K302002;Parent=BBa_K302013
BBa_K302015	.	non_covalent_binding_site	259	264	.	+	0	ID=annotation2077777;Name=predicted pairing;Parent=BBa_K302002
BBa_K302015	.	non_covalent_binding_site	236	241	.	+	0	ID=annotation2077775;Name=predicted pairing;Parent=BBa_K302002
BBa_K302015	.	non_covalent_binding_site	216	222	.	+	0	ID=annotation2077773;Name=predicted pairing;Parent=BBa_K302002
BBa_K302015	.	non_covalent_binding_site	225	233	.	+	0	ID=annotation2077774;Name=predicted pairing;Parent=BBa_K302002
BBa_K302015	.	non_covalent_binding_site	252	257	.	+	0	ID=annotation2077776;Name=predicted pairing;Parent=BBa_K302002
BBa_K302015	.	non_covalent_binding_site	282	287	.	+	0	ID=annotation2077778;Name=predicted pairing;Parent=BBa_K302002
BBa_K302015	.	non_covalent_binding_site	121	125	.	+	0	ID=annotation2077772;Name=predicted pairing;Parent=BBa_K302002
BBa_K302015	.	promoter	1	106	.	+	0	ID=BBa_K174004;Name=BBa_K174004;Parent=BBa_K302013
BBa_K302015	.	operator	81	100	.	+	0	ID=annotation2033093;Name=oid - Lac operator;Parent=BBa_K174004
BBa_K302015	.	promoter	43	80	.	+	0	ID=annotation2033094;Name=pspac promoter;Parent=BBa_K174004
BBa_K302015	.	terminator	313	407	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K302013
BBa_K302015	.	terminator	313	353	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K302015	.	polyA_site	340	353	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K302015	.	engineered_region	313	353	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K302015	.	stem_loop	320	339	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K302015	.	stop_codon	346	346	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K302015	.	terminator	362	407	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K302015	.	stem_loop	374	396	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K302015	.	engineered_region	362	407	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
>BBa_K302015
agaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtg
gaattgtgagcgctcacaattaagcttaacaaaagaaattaagcgttttcaaatttcaaagaaatggggaataagagat
gggtacgattgtttgccaagattgcaacgaagccattcattactttgaagatgagaaagtgacaacattatatggaact
tgctgcggacaatgtcaatgtcctgttgatgaagagtaaaaaaaagcatgcggcttaaagccgcatgctttttattcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggct
tttttattatttagaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtg
gcataatgtgtggaattgtgagcgctcacaattaagctttcttaaatgttgaggtggaatcacagatggataaaacgat
ttcggttattggaatgccaatggatttaggacaagcacgacgcggagtggatatgggcccgagtgccatccggtacgct
catctgatcgagaggctgtcagacatggggtatacggttgaagatctcggtgacattccgatcaatcgcgaaaaaatca
aaaatgacgaggaactgaaaaacctgaactccgttttggcgggaaatgaaaaactcgcgcaaaaggtcaacaaagtcat
tgaagagaaaaaattcccgcttgtcctgggcggtgaccacagtattgcgatcggcacgcttgcaggcacagcgaagcat
tacgataatctcggcgtcatctggtatgacgcgcacggcgatttgaatacacttgaaacttcaccatcgggcaatattc
acggcatgccgctcgcggtcagcctaggcattggccacgagtcactggttaaccttgaaggctacgcgcctaaaatcaa
accggaaaacgtcgtcatcattggcgcccggtcacttgatgaaggggagcgcaagtacattaaggaaagcggcatgaag
gtgtacacaatgcacgaaatcgatcgtcttggcatgacaaaggtcattgaagaaacccttgattatttatcagcatgtg
atggcgtccatctgagccttgatctggacggacttgatccgaacgacgcaccgggtgtcggaacccctgtcgtcggcgg
catcagctaccgggagagccatttggctatggaaatgctgtatgacgcaggcatcattacctcagccgaatttgttgag
gttaacccgatccttgatcacaaaaacaaaacgggcaaaacagcagtagagctcgtagaatccctgttagggaagaagc
tgctgtaataagaaaaccccctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatat
aaaaagccagattattaatccggcttttttattattt