BBa_K302015
type: ComponentDefinition
persistent identity: BBa K302015
display id: BBa_K302015
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG-inducible urea/urease
created: 2010-08-12T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: Urease
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 0
owner id: 6009
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: .
star: false
creator: Steven Woodhouse, Alan Koh, Harsh Sheth
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K302014
component: BBa_K302013
sequence annotation: BBa_K302013
sequence annotation: BBa_K302014
sequence: https://synbiohub.org/public/igem/BBa_K302015_sequence/1

BBa_K302014
type: Component
persistent identity: component2226688
display id: component2226688
version: 1
top level: BBa_K302015
definition: BBa_K302014
access: public

BBa_K302013
type: Component
persistent identity: component2226672
display id: component2226672
version: 1
top level: BBa_K302015
definition: BBa_K302013
access: public

BBa_K302013
type: SequenceAnnotation
persistent identity: annotation2226672
display id: annotation2226672
version: 1
top level: BBa_K302015
location: https://synbiohub.org/public/igem/BBa_K302015/annotation2226672/range2226672/1
component: BBa_K302013

https://synbiohub.org/public/igem/BBa_K302015/annotation2226672/range2226672/1
type: Range
persistent identity: range2226672
display id: range2226672
version: 1
top level: BBa_K302015
direction: direction/reverse
start: 1
end: 407
orientation: inline

BBa_K302014
type: SequenceAnnotation
persistent identity: annotation2226688
display id: annotation2226688
version: 1
top level: BBa_K302015
location: https://synbiohub.org/public/igem/BBa_K302015/annotation2226688/range2226688/1
component: BBa_K302014

https://synbiohub.org/public/igem/BBa_K302015/annotation2226688/range2226688/1
type: Range
persistent identity: range2226688
display id: range2226688
version: 1
top level: BBa_K302015
direction: direction/reverse
start: 408
end: 1538
orientation: inline

BBa_K302013
type: ComponentDefinition
persistent identity: BBa K302013
display id: BBa_K302013
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG-inducible L-arginine
created: 2010-08-11T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: Synthesised.. reference paper.
owned by: james
owned by: myers
mutable description: SR1 ..
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 0
owner id: 6009
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: No RBS needed.
star: false
creator: Steven Woodhouse, Alan Koh
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K174004
component: BBa_B0014
component: BBa_K302002
sequence annotation: BBa_K174004
sequence annotation: BBa_K302002
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K302013_sequence/1

BBa_K174004
type: Component
persistent identity: component2220646
display id: component2220646
version: 1
top level: BBa_K302013
definition: BBa_K174004
access: public

BBa_B0014
type: Component
persistent identity: component2220661
display id: component2220661
version: 1
top level: BBa_K302013
definition: BBa_B0014
access: public

BBa_K302002
type: Component
persistent identity: component2220654
display id: component2220654
version: 1
top level: BBa_K302013
definition: BBa_K302002
access: public

BBa_K174004
type: SequenceAnnotation
persistent identity: annotation2220646
display id: annotation2220646
version: 1
top level: BBa_K302013
location: https://synbiohub.org/public/igem/BBa_K302013/annotation2220646/range2220646/1
component: BBa_K174004

https://synbiohub.org/public/igem/BBa_K302013/annotation2220646/range2220646/1
type: Range
persistent identity: range2220646
display id: range2220646
version: 1
top level: BBa_K302013
direction: direction/reverse
start: 1
end: 106
orientation: inline

BBa_K302002
type: SequenceAnnotation
persistent identity: annotation2220654
display id: annotation2220654
version: 1
top level: BBa_K302013
location: https://synbiohub.org/public/igem/BBa_K302013/annotation2220654/range2220654/1
component: BBa_K302002

https://synbiohub.org/public/igem/BBa_K302013/annotation2220654/range2220654/1
type: Range
persistent identity: range2220654
display id: range2220654
version: 1
top level: BBa_K302013
direction: direction/reverse
start: 107
end: 312
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2220661
display id: annotation2220661
version: 1
top level: BBa_K302013
location: https://synbiohub.org/public/igem/BBa_K302013/annotation2220661/range2220661/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K302013/annotation2220661/range2220661/1
type: Range
persistent identity: range2220661
display id: range2220661
version: 1
top level: BBa_K302013
direction: direction/reverse
start: 313
end: 407
orientation: inline

BBa_K302014
type: ComponentDefinition
persistent identity: BBa K302014
display id: BBa_K302014
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG-inducible arginase
created: 2010-08-12T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: The ''rocF'' sequence was taken from ''Bacillus subtilis'' 168 genomic DNA, by PCR. Pspac oid,. .... assembled by the Gibson method...
owned by: james
owned by: myers
mutable description: IPTG-inducible arginase production for ''Bacillus subtilis''.
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 0
owner id: 6009
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Two EcoR1 restriction sites were removed from the ''rocF'' coding sequence.
star: false
creator: Alan Koh, Steven Woodhouse, Harsh Sheth
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K174004
component: BBa_B0014
component: BBa_K302000
sequence annotation: BBa_K174004
sequence annotation: BBa_K302000
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K302014_sequence/1

BBa_K174004
type: Component
persistent identity: component2220631
display id: component2220631
version: 1
top level: BBa_K302014
definition: BBa_K174004
access: public

BBa_B0014
type: Component
persistent identity: component2220643
display id: component2220643
version: 1
top level: BBa_K302014
definition: BBa_B0014
access: public

BBa_K302000
type: Component
persistent identity: component2220636
display id: component2220636
version: 1
top level: BBa_K302014
definition: BBa_K302000
access: public

BBa_K174004
type: SequenceAnnotation
persistent identity: annotation2220631
display id: annotation2220631
version: 1
top level: BBa_K302014
location: https://synbiohub.org/public/igem/BBa_K302014/annotation2220631/range2220631/1
component: BBa_K174004

https://synbiohub.org/public/igem/BBa_K302014/annotation2220631/range2220631/1
type: Range
persistent identity: range2220631
display id: range2220631
version: 1
top level: BBa_K302014
direction: direction/reverse
start: 1
end: 106
orientation: inline

BBa_K302000
type: SequenceAnnotation
persistent identity: annotation2220636
display id: annotation2220636
version: 1
top level: BBa_K302014
location: https://synbiohub.org/public/igem/BBa_K302014/annotation2220636/range2220636/1
component: BBa_K302000

https://synbiohub.org/public/igem/BBa_K302014/annotation2220636/range2220636/1
type: Range
persistent identity: range2220636
display id: range2220636
version: 1
top level: BBa_K302014
direction: direction/reverse
start: 107
end: 1036
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2220643
display id: annotation2220643
version: 1
top level: BBa_K302014
location: https://synbiohub.org/public/igem/BBa_K302014/annotation2220643/range2220643/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K302014/annotation2220643/range2220643/1
type: Range
persistent identity: range2220643
display id: range2220643
version: 1
top level: BBa_K302014
direction: direction/reverse
start: 1037
end: 1131
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_K174004
type: ComponentDefinition
persistent identity: BBa K174004
display id: BBa_K174004
version: 1
was generated by: iGEM to SBOL conversion
description: Pspac promoter
created: 2009-10-09T11:00:00Z
modified: 2015-05-08T01:10:58Z
mutable provenance: This sequence is taken from pmutin4 integration vector. pmutin2's lacI binding site was changed with the "oid" sequence to create pmutin4.
owned by: james
owned by: myers
mutable description: This promoter can be induced by IPTG. Wild type LacI binding site is replaced with a perfect palindromic "oid" operator sequence which increases the repression by LacI. This biobrick is an enhancement of Biobrick BBa_K090501 which has the sequence from pmutin2.
discontinued: false
dominant: false
experience: experience/Works
group u list: _277_
m user id: 0
owner id: 3942
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: pmutin2's genbank accession number is AF072806. Although there is no sequence information for pmutin4, it can be derived from pmutin2's sequence information by changing the O1 lacI binding site with the "oid" sequence.
star: false
creator: The Newcastle 2009 iGEM team
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: pspac promoter
sequence annotation: oid - Lac operator
sequence: https://synbiohub.org/public/igem/BBa_K174004_sequence/1

pspac promoter
type: SequenceAnnotation
persistent identity: annotation2033094
display id: annotation2033094
version: 1
top level: BBa_K174004
location: https://synbiohub.org/public/igem/BBa_K174004/annotation2033094/range2033094/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K174004/annotation2033094/range2033094/1
type: Range
persistent identity: range2033094
display id: range2033094
version: 1
top level: BBa_K174004
direction: direction/forward
start: 43
end: 80
orientation: inline

oid - Lac operator
type: SequenceAnnotation
persistent identity: annotation2033093
display id: annotation2033093
version: 1
top level: BBa_K174004
location: https://synbiohub.org/public/igem/BBa_K174004/annotation2033093/range2033093/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K174004/annotation2033093/range2033093/1
type: Range
persistent identity: range2033093
display id: range2033093
version: 1
top level: BBa_K174004
direction: direction/forward
start: 81
end: 100
orientation: inline

BBa_K302000
type: ComponentDefinition
persistent identity: BBa K302000
display id: BBa_K302000
version: 1
was generated by: iGEM to SBOL conversion
description: rocF RBS and coding sequence
created: 2010-08-11T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: ''Bacillus subtilis'' 168 genome.
owned by: james
owned by: myers
mutable description: BioBrick-compatible ''rocF'' coding sequence, which codes for the enzyme arginase.
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 0
owner id: 6009
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Removal of two EcoR1 sites.
star: false
creator: Alan Koh, Steven Woodhouse, Harsh Sheth
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: start
sequence annotation: Single base mutation to remove EcoR1 site
sequence annotation: Double stop
sequence: https://synbiohub.org/public/igem/BBa_K302000_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2077659
display id: annotation2077659
version: 1
top level: BBa_K302000
location: https://synbiohub.org/public/igem/BBa_K302000/annotation2077659/range2077659/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K302000/annotation2077659/range2077659/1
type: Range
persistent identity: range2077659
display id: range2077659
version: 1
top level: BBa_K302000
direction: direction/forward
start: 27
end: 29
orientation: inline

Single base mutation to remove EcoR1 site
type: SequenceAnnotation
persistent identity: annotation2077770
display id: annotation2077770
version: 1
top level: BBa_K302000
location: https://synbiohub.org/public/igem/BBa_K302000/annotation2077770/range2077770/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K302000/annotation2077770/range2077770/1
type: Range
persistent identity: range2077770
display id: range2077770
version: 1
top level: BBa_K302000
direction: direction/forward
start: 227
end: 227
orientation: inline

Single base mutation to remove EcoR1 site
type: SequenceAnnotation
persistent identity: annotation2077771
display id: annotation2077771
version: 1
top level: BBa_K302000
location: https://synbiohub.org/public/igem/BBa_K302000/annotation2077771/range2077771/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K302000/annotation2077771/range2077771/1
type: Range
persistent identity: range2077771
display id: range2077771
version: 1
top level: BBa_K302000
direction: direction/forward
start: 824
end: 824
orientation: inline

Double stop
type: SequenceAnnotation
persistent identity: annotation2077660
display id: annotation2077660
version: 1
top level: BBa_K302000
location: https://synbiohub.org/public/igem/BBa_K302000/annotation2077660/range2077660/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K302000/annotation2077660/range2077660/1
type: Range
persistent identity: range2077660
display id: range2077660
version: 1
top level: BBa_K302000
direction: direction/forward
start: 915
end: 919
orientation: inline

BBa_K302002
type: ComponentDefinition
persistent identity: BBa K302002
display id: BBa_K302002
version: 1
was generated by: iGEM to SBOL conversion
description: SR1 antisense RNA (L-arginine)
created: 2010-08-11T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: The sequence was taken from ''The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism'', Heidrich et al. 2006.
owned by: james
owned by: myers
mutable description: SR1 is an untranslated RNA which acts as the antisense to ''ahrC'' mRNA.
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 0
owner id: 6009
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No RBS required.
star: false
creator: Steven Woodhouse, Alan Koh
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: predicted pairing
sequence: https://synbiohub.org/public/igem/BBa_K302002_sequence/1

predicted pairing
type: SequenceAnnotation
persistent identity: annotation2077772
display id: annotation2077772
version: 1
top level: BBa_K302002
location: https://synbiohub.org/public/igem/BBa_K302002/annotation2077772/range2077772/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K302002/annotation2077772/range2077772/1
type: Range
persistent identity: range2077772
display id: range2077772
version: 1
top level: BBa_K302002
direction: direction/forward
start: 15
end: 19
orientation: inline

predicted pairing
type: SequenceAnnotation
persistent identity: annotation2077773
display id: annotation2077773
version: 1
top level: BBa_K302002
location: https://synbiohub.org/public/igem/BBa_K302002/annotation2077773/range2077773/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K302002/annotation2077773/range2077773/1
type: Range
persistent identity: range2077773
display id: range2077773
version: 1
top level: BBa_K302002
direction: direction/forward
start: 110
end: 116
orientation: inline

predicted pairing
type: SequenceAnnotation
persistent identity: annotation2077774
display id: annotation2077774
version: 1
top level: BBa_K302002
location: https://synbiohub.org/public/igem/BBa_K302002/annotation2077774/range2077774/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K302002/annotation2077774/range2077774/1
type: Range
persistent identity: range2077774
display id: range2077774
version: 1
top level: BBa_K302002
direction: direction/forward
start: 119
end: 127
orientation: inline

predicted pairing
type: SequenceAnnotation
persistent identity: annotation2077775
display id: annotation2077775
version: 1
top level: BBa_K302002
location: https://synbiohub.org/public/igem/BBa_K302002/annotation2077775/range2077775/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K302002/annotation2077775/range2077775/1
type: Range
persistent identity: range2077775
display id: range2077775
version: 1
top level: BBa_K302002
direction: direction/forward
start: 130
end: 135
orientation: inline

predicted pairing
type: SequenceAnnotation
persistent identity: annotation2077776
display id: annotation2077776
version: 1
top level: BBa_K302002
location: https://synbiohub.org/public/igem/BBa_K302002/annotation2077776/range2077776/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K302002/annotation2077776/range2077776/1
type: Range
persistent identity: range2077776
display id: range2077776
version: 1
top level: BBa_K302002
direction: direction/forward
start: 146
end: 151
orientation: inline

predicted pairing
type: SequenceAnnotation
persistent identity: annotation2077777
display id: annotation2077777
version: 1
top level: BBa_K302002
location: https://synbiohub.org/public/igem/BBa_K302002/annotation2077777/range2077777/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K302002/annotation2077777/range2077777/1
type: Range
persistent identity: range2077777
display id: range2077777
version: 1
top level: BBa_K302002
direction: direction/forward
start: 153
end: 158
orientation: inline

predicted pairing
type: SequenceAnnotation
persistent identity: annotation2077778
display id: annotation2077778
version: 1
top level: BBa_K302002
location: https://synbiohub.org/public/igem/BBa_K302002/annotation2077778/range2077778/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K302002/annotation2077778/range2077778/1
type: Range
persistent identity: range2077778
display id: range2077778
version: 1
top level: BBa_K302002
direction: direction/forward
start: 176
end: 181
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K302015_sequence/1
type: Sequence
persistent identity: BBa K302015 sequence
display id: BBa_K302015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcgctcacaattaagcttaacaaaagaaattaagcgttttcaaatttcaaagaaatggggaataagagatgggtacgattgtttgccaagattgcaacgaagccattcattactttgaagatgagaaagtgacaacattatatggaacttgctgcggacaatgtcaatgtcctgttgatgaagagtaaaaaaaagcatgcggcttaaagccgcatgctttttattcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttagaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcgctcacaattaagctttcttaaatgttgaggtggaatcacagatggataaaacgatttcggttattggaatgccaatggatttaggacaagcacgacgcggagtggatatgggcccgagtgccatccggtacgctcatctgatcgagaggctgtcagacatggggtatacggttgaagatctcggtgacattccgatcaatcgcgaaaaaatcaaaaatgacgaggaactgaaaaacctgaactccgttttggcgggaaatgaaaaactcgcgcaaaaggtcaacaaagtcattgaagagaaaaaattcccgcttgtcctgggcggtgaccacagtattgcgatcggcacgcttgcaggcacagcgaagcattacgataatctcggcgtcatctggtatgacgcgcacggcgatttgaatacacttgaaacttcaccatcgggcaatattcacggcatgccgctcgcggtcagcctaggcattggccacgagtcactggttaaccttgaaggctacgcgcctaaaatcaaaccggaaaacgtcgtcatcattggcgcccggtcacttgatgaaggggagcgcaagtacattaaggaaagcggcatgaaggtgtacacaatgcacgaaatcgatcgtcttggcatgacaaaggtcattgaagaaacccttgattatttatcagcatgtgatggcgtccatctgagccttgatctggacggacttgatccgaacgacgcaccgggtgtcggaacccctgtcgtcggcggcatcagctaccgggagagccatttggctatggaaatgctgtatgacgcaggcatcattacctcagccgaatttgttgaggttaacccgatccttgatcacaaaaacaaaacgggcaaaacagcagtagagctcgtagaatccctgttagggaagaagctgctgtaataagaaaaccccctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302013_sequence/1
type: Sequence
persistent identity: BBa K302013 sequence
display id: BBa_K302013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcgctcacaattaagcttaacaaaagaaattaagcgttttcaaatttcaaagaaatggggaataagagatgggtacgattgtttgccaagattgcaacgaagccattcattactttgaagatgagaaagtgacaacattatatggaacttgctgcggacaatgtcaatgtcctgttgatgaagagtaaaaaaaagcatgcggcttaaagccgcatgctttttattcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302014_sequence/1
type: Sequence
persistent identity: BBa K302014 sequence
display id: BBa_K302014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcgctcacaattaagctttcttaaatgttgaggtggaatcacagatggataaaacgatttcggttattggaatgccaatggatttaggacaagcacgacgcggagtggatatgggcccgagtgccatccggtacgctcatctgatcgagaggctgtcagacatggggtatacggttgaagatctcggtgacattccgatcaatcgcgaaaaaatcaaaaatgacgaggaactgaaaaacctgaactccgttttggcgggaaatgaaaaactcgcgcaaaaggtcaacaaagtcattgaagagaaaaaattcccgcttgtcctgggcggtgaccacagtattgcgatcggcacgcttgcaggcacagcgaagcattacgataatctcggcgtcatctggtatgacgcgcacggcgatttgaatacacttgaaacttcaccatcgggcaatattcacggcatgccgctcgcggtcagcctaggcattggccacgagtcactggttaaccttgaaggctacgcgcctaaaatcaaaccggaaaacgtcgtcatcattggcgcccggtcacttgatgaaggggagcgcaagtacattaaggaaagcggcatgaaggtgtacacaatgcacgaaatcgatcgtcttggcatgacaaaggtcattgaagaaacccttgattatttatcagcatgtgatggcgtccatctgagccttgatctggacggacttgatccgaacgacgcaccgggtgtcggaacccctgtcgtcggcggcatcagctaccgggagagccatttggctatggaaatgctgtatgacgcaggcatcattacctcagccgaatttgttgaggttaacccgatccttgatcacaaaaacaaaacgggcaaaacagcagtagagctcgtagaatccctgttagggaagaagctgctgtaataagaaaaccccctcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K174004_sequence/1
type: Sequence
persistent identity: BBa K174004 sequence
display id: BBa_K174004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcgctcacaattaagctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302000_sequence/1
type: Sequence
persistent identity: BBa K302000 sequence
display id: BBa_K302000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcttaaatgttgaggtggaatcacagatggataaaacgatttcggttattggaatgccaatggatttaggacaagcacgacgcggagtggatatgggcccgagtgccatccggtacgctcatctgatcgagaggctgtcagacatggggtatacggttgaagatctcggtgacattccgatcaatcgcgaaaaaatcaaaaatgacgaggaactgaaaaacctgaactccgttttggcgggaaatgaaaaactcgcgcaaaaggtcaacaaagtcattgaagagaaaaaattcccgcttgtcctgggcggtgaccacagtattgcgatcggcacgcttgcaggcacagcgaagcattacgataatctcggcgtcatctggtatgacgcgcacggcgatttgaatacacttgaaacttcaccatcgggcaatattcacggcatgccgctcgcggtcagcctaggcattggccacgagtcactggttaaccttgaaggctacgcgcctaaaatcaaaccggaaaacgtcgtcatcattggcgcccggtcacttgatgaaggggagcgcaagtacattaaggaaagcggcatgaaggtgtacacaatgcacgaaatcgatcgtcttggcatgacaaaggtcattgaagaaacccttgattatttatcagcatgtgatggcgtccatctgagccttgatctggacggacttgatccgaacgacgcaccgggtgtcggaacccctgtcgtcggcggcatcagctaccgggagagccatttggctatggaaatgctgtatgacgcaggcatcattacctcagccgaatttgttgaggttaacccgatccttgatcacaaaaacaaaacgggcaaaacagcagtagagctcgtagaatccctgttagggaagaagctgctgtaataagaaaaccccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302002_sequence/1
type: Sequence
persistent identity: BBa K302002 sequence
display id: BBa_K302002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaaaagaaattaagcgttttcaaatttcaaagaaatggggaataagagatgggtacgattgtttgccaagattgcaacgaagccattcattactttgaagatgagaaagtgacaacattatatggaacttgctgcggacaatgtcaatgtcctgttgatgaagagtaaaaaaaagcatgcggcttaaagccgcatgctttttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z