BBa_K302016
type: ComponentDefinition
persistent identity: BBa K302016
display id: BBa_K302016
version: 1
was generated by: iGEM to SBOL conversion
description: Swarming
created: 2010-08-11T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: Composite
owned by: james
owned by: myers
mutable description: Swarming
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 0
owner id: 6009
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: None.
star: false
creator: Harsh Sheth, Alan Koh, Steven Woodhouse
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_B0014
component: BBa_K143021
component: BBa_K302010
component: BBa_K302011
component: BBa_G0000
component: BBa_K143053
sequence annotation: BBa_K143053
sequence annotation: BBa_G0000
sequence annotation: BBa_K302010
sequence annotation: BBa_K143021
sequence annotation: BBa_K302011
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K302016_sequence/1

BBa_B0014
type: Component
persistent identity: component2227547
display id: component2227547
version: 1
top level: BBa_K302016
definition: BBa_B0014
access: public

BBa_K143021
type: Component
persistent identity: component2227537
display id: component2227537
version: 1
top level: BBa_K302016
definition: RBS-spoVG
access: public

BBa_K302010
type: Component
persistent identity: component2227535
display id: component2227535
version: 1
top level: BBa_K302016
definition: BBa_K302010
access: public

BBa_K302011
type: Component
persistent identity: component2227540
display id: component2227540
version: 1
top level: BBa_K302016
definition: BBa_K302011
access: public

BBa_G0000
type: Component
persistent identity: component2227538
display id: component2227538
version: 1
top level: BBa_K302016
definition: scar
access: public

BBa_G0000
type: Component
persistent identity: component2227533
display id: component2227533
version: 1
top level: BBa_K302016
definition: scar
access: public

BBa_K143053
type: Component
persistent identity: component2227532
display id: component2227532
version: 1
top level: BBa_K302016
definition: Pveg-spoVG
access: public

BBa_K143053
type: SequenceAnnotation
persistent identity: annotation2227532
display id: annotation2227532
version: 1
top level: BBa_K302016
location: https://synbiohub.org/public/igem/BBa_K302016/annotation2227532/range2227532/1
component: BBa_K143053

https://synbiohub.org/public/igem/BBa_K302016/annotation2227532/range2227532/1
type: Range
persistent identity: range2227532
display id: range2227532
version: 1
top level: BBa_K302016
direction: direction/reverse
start: 1
end: 117
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2227533
display id: annotation2227533
version: 1
top level: BBa_K302016
location: https://synbiohub.org/public/igem/BBa_K302016/annotation2227533/range2227533/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K302016/annotation2227533/range2227533/1
type: Range
persistent identity: range2227533
display id: range2227533
version: 1
top level: BBa_K302016
direction: direction/reverse
start: 118
end: 123
orientation: inline

BBa_K302010
type: SequenceAnnotation
persistent identity: annotation2227535
display id: annotation2227535
version: 1
top level: BBa_K302016
location: https://synbiohub.org/public/igem/BBa_K302016/annotation2227535/range2227535/1
component: BBa_K302010

https://synbiohub.org/public/igem/BBa_K302016/annotation2227535/range2227535/1
type: Range
persistent identity: range2227535
display id: range2227535
version: 1
top level: BBa_K302016
direction: direction/reverse
start: 124
end: 801
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2227537
display id: annotation2227537
version: 1
top level: BBa_K302016
location: https://synbiohub.org/public/igem/BBa_K302016/annotation2227537/range2227537/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K302016/annotation2227537/range2227537/1
type: Range
persistent identity: range2227537
display id: range2227537
version: 1
top level: BBa_K302016
direction: direction/reverse
start: 802
end: 813
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2227538
display id: annotation2227538
version: 1
top level: BBa_K302016
location: https://synbiohub.org/public/igem/BBa_K302016/annotation2227538/range2227538/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K302016/annotation2227538/range2227538/1
type: Range
persistent identity: range2227538
display id: range2227538
version: 1
top level: BBa_K302016
direction: direction/reverse
start: 814
end: 819
orientation: inline

BBa_K302011
type: SequenceAnnotation
persistent identity: annotation2227540
display id: annotation2227540
version: 1
top level: BBa_K302016
location: https://synbiohub.org/public/igem/BBa_K302016/annotation2227540/range2227540/1
component: BBa_K302011

https://synbiohub.org/public/igem/BBa_K302016/annotation2227540/range2227540/1
type: Range
persistent identity: range2227540
display id: range2227540
version: 1
top level: BBa_K302016
direction: direction/reverse
start: 820
end: 1251
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2227547
display id: annotation2227547
version: 1
top level: BBa_K302016
location: https://synbiohub.org/public/igem/BBa_K302016/annotation2227547/range2227547/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K302016/annotation2227547/range2227547/1
type: Range
persistent identity: range2227547
display id: range2227547
version: 1
top level: BBa_K302016
direction: direction/reverse
start: 1252
end: 1346
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa G0000
display id: BBa_G0000
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/XbaI scar for RBS-CDS junctions
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: SpeI/XbaI scar
owned by: james
owned by: myers
mutable description: This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a shorter scar to ensure proper spacing between the RBS and CDS.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0000_sequence/1

RBS-spoVG
type: ComponentDefinition
persistent identity: BBa K143021
display id: BBa_K143021
version: 1
was generated by: iGEM to SBOL conversion
description: SpoVG ribosome binding site (RBS) for B. subtilis
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143021_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975997
display id: annotation1975997
version: 1
top level: RBS-spoVG
location: https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
type: Range
persistent identity: range1975997
display id: range1975997
version: 1
top level: RBS-spoVG
direction: direction/forward
start: 1
end: 12
orientation: inline

Pveg-spoVG
type: ComponentDefinition
persistent identity: BBa K143053
display id: BBa_K143053
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Pveg and RBS spoVG for B. subtilis
created: 2008-10-07T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: Pveg-spoVG was synthesised by GeneArt
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive promoter veg(<bbpart>BBa_K143012</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''. Pveg-spoVG can be used in the context of a '''Ribosomes per second''' (RiPS) output generator '''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence of Pveg was obtained from the DBTBS1 and RBS-spoVG were obtained from papers2 and the sequence synthesised by GeneArt
star: true
creator: Chris Hirst
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K143012
component: BBa_K143021
sequence annotation: BBa_K143012
sequence annotation: BBa_K143021
sequence: https://synbiohub.org/public/igem/BBa_K143053_sequence/1

BBa_K143012
type: Component
persistent identity: component1979395
display id: component1979395
version: 1
top level: Pveg-spoVG
definition: Pveg
access: public

BBa_K143021
type: Component
persistent identity: component1979397
display id: component1979397
version: 1
top level: Pveg-spoVG
definition: RBS-spoVG
access: public

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation1979395
display id: annotation1979395
version: 1
top level: Pveg-spoVG
location: https://synbiohub.org/public/igem/BBa_K143053/annotation1979395/range1979395/1
component: BBa_K143012

https://synbiohub.org/public/igem/BBa_K143053/annotation1979395/range1979395/1
type: Range
persistent identity: range1979395
display id: range1979395
version: 1
top level: Pveg-spoVG
direction: direction/reverse
start: 1
end: 97
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation1979397
display id: annotation1979397
version: 1
top level: Pveg-spoVG
location: https://synbiohub.org/public/igem/BBa_K143053/annotation1979397/range1979397/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K143053/annotation1979397/range1979397/1
type: Range
persistent identity: range1979397
display id: range1979397
version: 1
top level: Pveg-spoVG
direction: direction/reverse
start: 106
end: 117
orientation: inline

BBa_K302010
type: ComponentDefinition
persistent identity: BBa K302010
display id: BBa_K302010
version: 1
was generated by: iGEM to SBOL conversion
description: sfp
created: 2010-08-11T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: ''Bacillus subtilis'' 3610 genomic DNA, by PCR.
owned by: james
owned by: myers
mutable description: ''Bacillus subtilis'' 168 is unable to produce surfactin, a natural surfactant which helps in reducing surface tension and aids bacteria to swarm on a solid or semi solid medium. The ''sfp'' gene restores the capability to produce surfactin.
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 0
owner id: 6009
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Second TAA stop codon was added.
star: false
creator: Harsh Sheth, Alan Koh, Steven Woodhouse
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Added TAA
sequence: https://synbiohub.org/public/igem/BBa_K302010_sequence/1

Added TAA
type: SequenceAnnotation
persistent identity: annotation2080974
display id: annotation2080974
version: 1
top level: BBa_K302010
location: https://synbiohub.org/public/igem/BBa_K302010/annotation2080974/range2080974/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K302010/annotation2080974/range2080974/1
type: Range
persistent identity: range2080974
display id: range2080974
version: 1
top level: BBa_K302010
direction: direction/forward
start: 676
end: 678
orientation: inline

BBa_K302011
type: ComponentDefinition
persistent identity: BBa K302011
display id: BBa_K302011
version: 1
was generated by: iGEM to SBOL conversion
description: swrA
created: 2010-08-11T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: ''Bacillius subtilis'' 3610 genomic DNA, by PCR.
owned by: james
owned by: myers
mutable description: ''Bacillus subtilis'' 168 is unable to biosynthesise flagella. The ''swrA'' gene restores this capability.
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 0
owner id: 6009
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Extra TAA stop codon added.
star: false
creator: Harsh Sheth, Alan Koh, Steven Woodhouse
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Added TAA
sequence: https://synbiohub.org/public/igem/BBa_K302011_sequence/1

Added TAA
type: SequenceAnnotation
persistent identity: annotation2077615
display id: annotation2077615
version: 1
top level: BBa_K302011
location: https://synbiohub.org/public/igem/BBa_K302011/annotation2077615/range2077615/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K302011/annotation2077615/range2077615/1
type: Range
persistent identity: range2077615
display id: range2077615
version: 1
top level: BBa_K302011
direction: direction/forward
start: 430
end: 432
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

Pveg
type: ComponentDefinition
persistent identity: BBa K143012
display id: BBa_K143012
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter veg a constitutive promoter for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor#1. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
star: false
creator: James Chappell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143012_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation1975704
display id: annotation1975704
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
type: Range
persistent identity: range1975704
display id: range1975704
version: 1
top level: Pveg
direction: direction/forward
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975705
display id: annotation1975705
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
type: Range
persistent identity: range1975705
display id: range1975705
version: 1
top level: Pveg
direction: direction/forward
start: 86
end: 91
orientation: inline

https://synbiohub.org/public/igem/BBa_K302016_sequence/1
type: Sequence
persistent identity: BBa K302016 sequence
display id: BBa_K302016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaagatttacggaatttatatggaccgcccgctttcacaggaagaaaatgaacggttcatgtctttcatatcacctgaaaaacgggagaaatgccggagattttatcataaagaagatgctcaccgcaccctgctgggagatgtgctcgttcgctcagtcataagcaggcagtatcagttggacaaatccgatatccgctttagcacgcaggaatacgggaagccgtgcatccctgatcttcccgacgctcatttcaacatttctcactccggacgctgggtcatttgcgcgtttgattcacagccgatcggcatagatatcgaaaaaacgaaaccgatcagccttgagatcgccaagcgcttcttttcaaaaacagagtacagcgaccttttagcaaaagacaaggacgagcagacagactatttttatcatctatggtcaatgaaagaaagctttatcaaacaggaaggcaaaggcttatcgcttccgcttgattccttttcagtgcgcctgcaccaggacggacaagtatccattgagcttccggacagccattccccatgctatatcaaaacgtatgaggtcgatcccggctacaaaatggctgtatgcgccgtacaccctgatttccccgaggatatcacaatggtctcgtacgaagagcttttataataaaaaggtggtgaatactagatgcttgttagcactccagtactgctgcttatactggcccgtgcttatctgtataaagaaaatgggggttcacaattgaagagggcaagtattgtgcgtgaaaaaaaatattatgaattagtggaacaattaaaagacagaacacaagacgtaacattttcagctacaaaagcactaagtcttcttatgctgttcagcagatatttggtcaattacaccaatgtcgaatcagtaaatgacattaatgaggaatgcgccaaacattattttaactacttaatgaaaaaccataagcgattaggaattaatctgacagatataaaaaggtcgatgcatctaatcagcgggttattggatgtggatgtaaaccactatttaaaggatttttcactatcgaatgtcacgctgtggatgacgcaagagagataataatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0000_sequence/1
type: Sequence
persistent identity: BBa G0000 sequence
display id: BBa_G0000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143021_sequence/1
type: Sequence
persistent identity: BBa K143021 sequence
display id: BBa_K143021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggtggtgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143053_sequence/1
type: Sequence
persistent identity: BBa K143053 sequence
display id: BBa_K143053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302010_sequence/1
type: Sequence
persistent identity: BBa K302010 sequence
display id: BBa_K302010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaagatttacggaatttatatggaccgcccgctttcacaggaagaaaatgaacggttcatgtctttcatatcacctgaaaaacgggagaaatgccggagattttatcataaagaagatgctcaccgcaccctgctgggagatgtgctcgttcgctcagtcataagcaggcagtatcagttggacaaatccgatatccgctttagcacgcaggaatacgggaagccgtgcatccctgatcttcccgacgctcatttcaacatttctcactccggacgctgggtcatttgcgcgtttgattcacagccgatcggcatagatatcgaaaaaacgaaaccgatcagccttgagatcgccaagcgcttcttttcaaaaacagagtacagcgaccttttagcaaaagacaaggacgagcagacagactatttttatcatctatggtcaatgaaagaaagctttatcaaacaggaaggcaaaggcttatcgcttccgcttgattccttttcagtgcgcctgcaccaggacggacaagtatccattgagcttccggacagccattccccatgctatatcaaaacgtatgaggtcgatcccggctacaaaatggctgtatgcgccgtacaccctgatttccccgaggatatcacaatggtctcgtacgaagagcttttataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302011_sequence/1
type: Sequence
persistent identity: BBa K302011 sequence
display id: BBa_K302011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcttgttagcactccagtactgctgcttatactggcccgtgcttatctgtataaagaaaatgggggttcacaattgaagagggcaagtattgtgcgtgaaaaaaaatattatgaattagtggaacaattaaaagacagaacacaagacgtaacattttcagctacaaaagcactaagtcttcttatgctgttcagcagatatttggtcaattacaccaatgtcgaatcagtaaatgacattaatgaggaatgcgccaaacattattttaactacttaatgaaaaaccataagcgattaggaattaatctgacagatataaaaaggtcgatgcatctaatcagcgggttattggatgtggatgtaaaccactatttaaaggatttttcactatcgaatgtcacgctgtggatgacgcaagagagataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143012_sequence/1
type: Sequence
persistent identity: BBa K143012 sequence
display id: BBa_K143012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z