LOCUS       BBa_K302018             2965 bp    DNA     linear       22-Dec-2024
DEFINITION  Subtilin immunity
ACCESSION   BBa_K302018
VERSION     BBa_K302018.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A -10
     promoter        1..97
                     /label=BBa_K143012
     misc_feature    98..109
                     /label=BBa_K143021
     RBS             98..109
                     /label=spoVG
     misc_feature    110..115
                     /label=BBa_G0000
     misc_feature    110..115
                     /label=BioBrick assembly scar
     misc_feature    116..613
                     /label=BBa_K302006
     CDS             116..613
                     /label=spaI
     misc_feature    600..1343
                     /label=BBa_K302007
     CDS             600..1343
                     /label=spaF
     misc_feature    1340..2094
                     /label=BBa_K302008
     CDS             1340..2094
                     /label=spaE
     misc_feature    2256..2870
                     /label=BBa_K302009
     CDS             2256..2870
                     /label=spaG
     misc_feature    98..2870
                     /label=BBa_K302017
     stem_loop       2878..2897
                     /label=T7 TE
     misc_feature    2904..2904
                     /label=stop
     polyA_site      2898..2911
                     /label=polya
     misc_feature    2871..2911
                     /label=BBa_B0012
     terminator      2871..2911
                     /label=BBa_B0012
     stem_loop       2932..2954
                     /label=stem_loop
     misc_feature    2920..2965
                     /label=BBa_B0011
     terminator      2920..2965
                     /label=BBa_B0011
     terminator      2871..2965
                     /label=BBa_B0014
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgtaaa ggtggtgaat actagatgtt
      121 gtttttgaaa agaagtgtta acattttagg ctgttttatt gttttattta tgttatctgc
      181 ttgtcaatca ttaacaaagt ttaaagataa agttgttgaa gaaacaaaaa aaacaatgca
      241 ttttactgat gataacgaaa acgatacatc ggaaacaatg gagagcctga ttgataaggg
      301 aaaacttgat caagtggtct atgacgatca actttatcat ctgaaagaga aagttgatga
      361 agacaaaaaa ggaaaagtaa taggggcaat aggccaaact ttctttgtag atggtgatgg
      421 aaagagatgg tcggaagaag aattgaaaga accgtatatc tctaataacc cggatgaaat
      481 tagagagaaa aaaccattac gctatgggaa ggtttatagt acaaacgaag attctgatgc
      541 caaagatgaa atcattgttg agtttaatcg tgagtattat cgagcggttt taataaagaa
      601 tgaaaaggaa taagggagag tgtgacatga gtacagtctt attagaaacc aaaagtctta
      661 caaaaaagtt tggcaaagag acagctgttg cagatgtttc attaactgtt caaaaaaaca
      721 gcatttatgg actgttggga ccaaatggag ctggaaaatc gacaacgctt aaaattttaa
      781 caggaatttt acggaaaact tcaggtgaaa ttctctttga tggacataaa tggaaaagat
      841 ctgatctgca aactatcgga tcgttaatag aagctccgcc tctttacgat aatttaactg
      901 catttgaaaa tgtgaaagta catgccacgc tgctcggtct ttccgaagat cgcatacaca
      961 gtgtgctaaa gactgtggat atgcagcatg ctggaaagaa aagagcaggc caattttcga
     1021 tggggatgag gcagcggctt ggaattgcaa tcgccttatt aaaccatcct aaactattaa
     1081 ttcttgatga acctacaaac gggttagatc caataggtat acaagagctc agagacctta
     1141 ttcgctcatt tcctcaggaa ggtataacgg tgattttatc tagtcacatc ctttcggaag
     1201 tcgagcaaat agcagatcat attgggatta tcatcgacgg tcatcttggg taccagggtg
     1261 aaataaacaa ggctgatgat ttggagcact tgtttatgga agttgtaaag tcaactcgtg
     1321 aaaaagaagg tgtaaaaaga tgataaatta tataaaagcc gaaaacctaa agtttaagag
     1381 gacgttttcc agaaaaatga ttatctttgt agctcttctt aatataggtt tttctttttt
     1441 aatgaacact caattttttg taccaggaac ttataactgg tggtctatta tatttatgcc
     1501 agtgatgatt gctttatttt gttctttatc tcatcaaaaa gaaaaaaagg cctctcatta
     1561 taacggtacg tattcattgc ctatagatct agggaaatta tggtacgcta agattattat
     1621 tattgcaatt tattcactat tatctcaggt tgtattttta gtttttatgt tactcatggg
     1681 attcgttata gctgattttt ccatcatttc gccttctata gttgccgcga gcttattgct
     1741 ttggttgaca agtctttggc aaattccgct ctgtttattt gtggcaaaaa agtggggatt
     1801 tacaatggcg gtaatgttaa atttcatagg gacgctagtt ctcggtgtta tgccggcatc
     1861 tcgtgccttt tggtggttca ttccttggag ctggccaatt cgaatgatgt gtccaaccat
     1921 tggtatacat cctaacggtt tgctgctcga aaacaatgat cctttgttga gttggatggt
     1981 tgtgcctcct gccatagtga tttcaatttt attttttttg atactggcat ttctgaccag
     2041 ccgttcgttt gccccaacaa ctgattacaa aatgaataag cgggaggcga tgtaaggtga
     2101 aattgctttt gaggtgttta caagctgatt tccagaagag caagcacacc tcttttttgt
     2161 ggctgcatct tatcattcct atcgtttgtt cagttatatt gattttgtat ttttacggaa
     2221 gagatcaagt ggaatttcac ctttataagt cttttatgga agctatcgca gttgcattac
     2281 cgctgctcat cggggtgcta tgtggaatga ctgcatcctt agaagaacag gcagggaaat
     2341 ttcatgtatt actagggagc acatctccga aaatcgttgc ttatgtgagt aaagtgttca
     2401 tgctgctttt cttggagatt tgctctattg gtttagcact attcatctat ttcattggat
     2461 taaagtttat tttaaaagtg ccggatttat cttacgatat ttttataatg ggtggtgcct
     2521 ggctggttgc aggcagtgta gtcctgtatt gtatttactt ttttatcagt tgtatgtttg
     2581 gaatgggggc ttctgtcctg ataggaggag caggcctgct catgacagct ctcatgaaca
     2641 ctggattagg ggatgctata tggaaattta acccgtgggc ctgggggatt agattgtcaa
     2701 gtttaaatga agtacttcac tttaaagaag taaatcaagg gcatcatcct ttattaatgc
     2761 atgaaataaa ttcaggggta ttgatgatgg tttttggtat aatatgtaca atcattgcca
     2821 gtcttatatg gtttagtaaa tgggaaggca gaaaaacgtt tgaataataa tcacactggc
     2881 tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa aaagccagat
     2941 tattaatccg gcttttttat tattt
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