BBa_K302017 1 BBa_K302017 spaIFEG cluster 2010-08-17T11:00:00Z 2015-05-08T01:11:52Z Gene bank Coding sequences and RBS' for spaIFEG cluster. false false _442_ 0 6345 9 Not in stock false SpaI uses spoVG RBS (BBa_K143021), spaFEG uses native RBS (spaF and spaE RBS are in the coding sequence of preceding protein coding sequence). SpaG has had its TAG stop codon replaced with TAATAA. false Steven Woodhouse, Alan Koh, Rachel May Boyd, Philip Hall annotation2078327 1 spoVG range2078327 1 1 12 annotation2078332 1 BBa_K302007 range2078332 1 503 1246 annotation2078336 1 BBa_K302009 range2078336 1 2159 2773 annotation2078331 1 spaI range2078331 1 19 516 annotation2078333 1 spaF range2078333 1 503 1246 annotation2078337 1 spaG range2078337 1 2159 2773 annotation2078330 1 BBa_K302006 range2078330 1 19 516 annotation2078329 1 BioBrick assembly scar range2078329 1 13 18 annotation2078335 1 spaE range2078335 1 1243 1997 annotation2078328 1 BBa_G0000 range2078328 1 13 18 annotation2078326 1 BBa_K143021 range2078326 1 1 12 annotation2078334 1 BBa_K302008 range2078334 1 1243 1997 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation1683 1 stem_loop range1683 1 13 35 annotation7019 1 BBa_B0011 range7019 1 1 46 BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939311 1 BBa_B0011 component939303 1 BBa_B0012 annotation939311 1 BBa_B0011 range939311 1 50 95 annotation939303 1 BBa_B0012 range939303 1 1 41 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 BBa_K143012 1 Pveg Promoter veg a constitutive promoter for B. subtilis 2008-09-10T11:00:00Z 2015-05-08T01:10:23Z The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database<cite>#3</cite> then a section containing the binding site synthesised by Geneart. Released HQ 2013 Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor<cite>#1</cite>. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes<cite>#2</cite>, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how<cite>#3</cite>. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator. false true _199_ 0 2090 9 In stock false The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence. false James Chappell annotation1975704 1 Sigma A-35 range1975704 1 63 68 annotation1975705 1 Sigma A -10 range1975705 1 86 91 BBa_K302018 1 BBa_K302018 Subtilin immunity 2010-08-17T11:00:00Z 2015-05-08T01:11:52Z Gene Bank Coding sequences for spaIFEG cluster. Device uses Pveg/spoVG promoter/RBS (BBa_K143053) and double terminator (BBa_B0014). This device will confer immunity to subtilin lantibiotic. false false _442_ 0 6345 9 Not in stock true SpaI uses spoVG RBS (BBa_K143021), spaFEG uses native RBS (spaF and spaE RBS are in the coding sequence of preceding protein coding sequence). SpaG has had its TAG stop codon replaced with TAATAA. false Steven Woodhouse, Alan Koh, Rachel May Boyd, Philip Hall component2220684 1 BBa_B0014 component2220664 1 BBa_K143012 component2220677 1 BBa_K302017 annotation2220664 1 BBa_K143012 range2220664 1 1 97 annotation2220684 1 BBa_B0014 range2220684 1 2871 2965 annotation2220677 1 BBa_K302017 range2220677 1 98 2870 BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K302017_sequence 1 aaaggtggtgaatactagatgttgtttttgaaaagaagtgttaacattttaggctgttttattgttttatttatgttatctgcttgtcaatcattaacaaagtttaaagataaagttgttgaagaaacaaaaaaaacaatgcattttactgatgataacgaaaacgatacatcggaaacaatggagagcctgattgataagggaaaacttgatcaagtggtctatgacgatcaactttatcatctgaaagagaaagttgatgaagacaaaaaaggaaaagtaataggggcaataggccaaactttctttgtagatggtgatggaaagagatggtcggaagaagaattgaaagaaccgtatatctctaataacccggatgaaattagagagaaaaaaccattacgctatgggaaggtttatagtacaaacgaagattctgatgccaaagatgaaatcattgttgagtttaatcgtgagtattatcgagcggttttaataaagaatgaaaaggaataagggagagtgtgacatgagtacagtcttattagaaaccaaaagtcttacaaaaaagtttggcaaagagacagctgttgcagatgtttcattaactgttcaaaaaaacagcatttatggactgttgggaccaaatggagctggaaaatcgacaacgcttaaaattttaacaggaattttacggaaaacttcaggtgaaattctctttgatggacataaatggaaaagatctgatctgcaaactatcggatcgttaatagaagctccgcctctttacgataatttaactgcatttgaaaatgtgaaagtacatgccacgctgctcggtctttccgaagatcgcatacacagtgtgctaaagactgtggatatgcagcatgctggaaagaaaagagcaggccaattttcgatggggatgaggcagcggcttggaattgcaatcgccttattaaaccatcctaaactattaattcttgatgaacctacaaacgggttagatccaataggtatacaagagctcagagaccttattcgctcatttcctcaggaaggtataacggtgattttatctagtcacatcctttcggaagtcgagcaaatagcagatcatattgggattatcatcgacggtcatcttgggtaccagggtgaaataaacaaggctgatgatttggagcacttgtttatggaagttgtaaagtcaactcgtgaaaaagaaggtgtaaaaagatgataaattatataaaagccgaaaacctaaagtttaagaggacgttttccagaaaaatgattatctttgtagctcttcttaatataggtttttcttttttaatgaacactcaattttttgtaccaggaacttataactggtggtctattatatttatgccagtgatgattgctttattttgttctttatctcatcaaaaagaaaaaaaggcctctcattataacggtacgtattcattgcctatagatctagggaaattatggtacgctaagattattattattgcaatttattcactattatctcaggttgtatttttagtttttatgttactcatgggattcgttatagctgatttttccatcatttcgccttctatagttgccgcgagcttattgctttggttgacaagtctttggcaaattccgctctgtttatttgtggcaaaaaagtggggatttacaatggcggtaatgttaaatttcatagggacgctagttctcggtgttatgccggcatctcgtgccttttggtggttcattccttggagctggccaattcgaatgatgtgtccaaccattggtatacatcctaacggtttgctgctcgaaaacaatgatcctttgttgagttggatggttgtgcctcctgccatagtgatttcaattttattttttttgatactggcatttctgaccagccgttcgtttgccccaacaactgattacaaaatgaataagcgggaggcgatgtaaggtgaaattgcttttgaggtgtttacaagctgatttccagaagagcaagcacacctcttttttgtggctgcatcttatcattcctatcgtttgttcagttatattgattttgtatttttacggaagagatcaagtggaatttcacctttataagtcttttatggaagctatcgcagttgcattaccgctgctcatcggggtgctatgtggaatgactgcatccttagaagaacaggcagggaaatttcatgtattactagggagcacatctccgaaaatcgttgcttatgtgagtaaagtgttcatgctgcttttcttggagatttgctctattggtttagcactattcatctatttcattggattaaagtttattttaaaagtgccggatttatcttacgatatttttataatgggtggtgcctggctggttgcaggcagtgtagtcctgtattgtatttacttttttatcagttgtatgtttggaatgggggcttctgtcctgataggaggagcaggcctgctcatgacagctctcatgaacactggattaggggatgctatatggaaatttaacccgtgggcctgggggattagattgtcaagtttaaatgaagtacttcactttaaagaagtaaatcaagggcatcatcctttattaatgcatgaaataaattcaggggtattgatgatggtttttggtataatatgtacaatcattgccagtcttatatggtttagtaaatgggaaggcagaaaaacgtttgaataataa BBa_K143012_sequence 1 aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt BBa_K302018_sequence 1 aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtaaaggtggtgaatactagatgttgtttttgaaaagaagtgttaacattttaggctgttttattgttttatttatgttatctgcttgtcaatcattaacaaagtttaaagataaagttgttgaagaaacaaaaaaaacaatgcattttactgatgataacgaaaacgatacatcggaaacaatggagagcctgattgataagggaaaacttgatcaagtggtctatgacgatcaactttatcatctgaaagagaaagttgatgaagacaaaaaaggaaaagtaataggggcaataggccaaactttctttgtagatggtgatggaaagagatggtcggaagaagaattgaaagaaccgtatatctctaataacccggatgaaattagagagaaaaaaccattacgctatgggaaggtttatagtacaaacgaagattctgatgccaaagatgaaatcattgttgagtttaatcgtgagtattatcgagcggttttaataaagaatgaaaaggaataagggagagtgtgacatgagtacagtcttattagaaaccaaaagtcttacaaaaaagtttggcaaagagacagctgttgcagatgtttcattaactgttcaaaaaaacagcatttatggactgttgggaccaaatggagctggaaaatcgacaacgcttaaaattttaacaggaattttacggaaaacttcaggtgaaattctctttgatggacataaatggaaaagatctgatctgcaaactatcggatcgttaatagaagctccgcctctttacgataatttaactgcatttgaaaatgtgaaagtacatgccacgctgctcggtctttccgaagatcgcatacacagtgtgctaaagactgtggatatgcagcatgctggaaagaaaagagcaggccaattttcgatggggatgaggcagcggcttggaattgcaatcgccttattaaaccatcctaaactattaattcttgatgaacctacaaacgggttagatccaataggtatacaagagctcagagaccttattcgctcatttcctcaggaaggtataacggtgattttatctagtcacatcctttcggaagtcgagcaaatagcagatcatattgggattatcatcgacggtcatcttgggtaccagggtgaaataaacaaggctgatgatttggagcacttgtttatggaagttgtaaagtcaactcgtgaaaaagaaggtgtaaaaagatgataaattatataaaagccgaaaacctaaagtttaagaggacgttttccagaaaaatgattatctttgtagctcttcttaatataggtttttcttttttaatgaacactcaattttttgtaccaggaacttataactggtggtctattatatttatgccagtgatgattgctttattttgttctttatctcatcaaaaagaaaaaaaggcctctcattataacggtacgtattcattgcctatagatctagggaaattatggtacgctaagattattattattgcaatttattcactattatctcaggttgtatttttagtttttatgttactcatgggattcgttatagctgatttttccatcatttcgccttctatagttgccgcgagcttattgctttggttgacaagtctttggcaaattccgctctgtttatttgtggcaaaaaagtggggatttacaatggcggtaatgttaaatttcatagggacgctagttctcggtgttatgccggcatctcgtgccttttggtggttcattccttggagctggccaattcgaatgatgtgtccaaccattggtatacatcctaacggtttgctgctcgaaaacaatgatcctttgttgagttggatggttgtgcctcctgccatagtgatttcaattttattttttttgatactggcatttctgaccagccgttcgtttgccccaacaactgattacaaaatgaataagcgggaggcgatgtaaggtgaaattgcttttgaggtgtttacaagctgatttccagaagagcaagcacacctcttttttgtggctgcatcttatcattcctatcgtttgttcagttatattgattttgtatttttacggaagagatcaagtggaatttcacctttataagtcttttatggaagctatcgcagttgcattaccgctgctcatcggggtgctatgtggaatgactgcatccttagaagaacaggcagggaaatttcatgtattactagggagcacatctccgaaaatcgttgcttatgtgagtaaagtgttcatgctgcttttcttggagatttgctctattggtttagcactattcatctatttcattggattaaagtttattttaaaagtgccggatttatcttacgatatttttataatgggtggtgcctggctggttgcaggcagtgtagtcctgtattgtatttacttttttatcagttgtatgtttggaatgggggcttctgtcctgataggaggagcaggcctgctcatgacagctctcatgaacactggattaggggatgctatatggaaatttaacccgtgggcctgggggattagattgtcaagtttaaatgaagtacttcactttaaagaagtaaatcaagggcatcatcctttattaatgcatgaaataaattcaggggtattgatgatggtttttggtataatatgtacaatcattgccagtcttatatggtttagtaaatgggaaggcagaaaaacgtttgaataataatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z