BBa_K302017
1
BBa_K302017
spaIFEG cluster
2010-08-17T11:00:00Z
2015-05-08T01:11:52Z
Gene bank
Coding sequences and RBS' for spaIFEG cluster.
false
false
_442_
0
6345
9
Not in stock
false
SpaI uses spoVG RBS (BBa_K143021), spaFEG uses native RBS (spaF and spaE RBS are in the coding sequence of preceding protein coding sequence). SpaG has had its TAG stop codon replaced with TAATAA.
false
Steven Woodhouse, Alan Koh, Rachel May Boyd, Philip Hall
annotation2078327
1
spoVG
range2078327
1
1
12
annotation2078332
1
BBa_K302007
range2078332
1
503
1246
annotation2078336
1
BBa_K302009
range2078336
1
2159
2773
annotation2078331
1
spaI
range2078331
1
19
516
annotation2078333
1
spaF
range2078333
1
503
1246
annotation2078337
1
spaG
range2078337
1
2159
2773
annotation2078330
1
BBa_K302006
range2078330
1
19
516
annotation2078329
1
BioBrick assembly scar
range2078329
1
13
18
annotation2078335
1
spaE
range2078335
1
1243
1997
annotation2078328
1
BBa_G0000
range2078328
1
13
18
annotation2078326
1
BBa_K143021
range2078326
1
1
12
annotation2078334
1
BBa_K302008
range2078334
1
1243
1997
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939311
1
BBa_B0011
component939303
1
BBa_B0012
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
BBa_K143012
1
Pveg
Promoter veg a constitutive promoter for B. subtilis
2008-09-10T11:00:00Z
2015-05-08T01:10:23Z
The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database<cite>#3</cite> then a section containing the binding site synthesised by Geneart.
Released HQ 2013
Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor<cite>#1</cite>. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes<cite>#2</cite>, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how<cite>#3</cite>. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
false
true
_199_
0
2090
9
In stock
false
The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
false
James Chappell
annotation1975704
1
Sigma A-35
range1975704
1
63
68
annotation1975705
1
Sigma A -10
range1975705
1
86
91
BBa_K302018
1
BBa_K302018
Subtilin immunity
2010-08-17T11:00:00Z
2015-05-08T01:11:52Z
Gene Bank
Coding sequences for spaIFEG cluster. Device uses Pveg/spoVG promoter/RBS (BBa_K143053) and double terminator (BBa_B0014). This device will confer immunity to subtilin lantibiotic.
false
false
_442_
0
6345
9
Not in stock
true
SpaI uses spoVG RBS (BBa_K143021), spaFEG uses native RBS (spaF and spaE RBS are in the coding sequence of preceding protein coding sequence). SpaG has had its TAG stop codon replaced with TAATAA.
false
Steven Woodhouse, Alan Koh, Rachel May Boyd, Philip Hall
component2220684
1
BBa_B0014
component2220664
1
BBa_K143012
component2220677
1
BBa_K302017
annotation2220664
1
BBa_K143012
range2220664
1
1
97
annotation2220684
1
BBa_B0014
range2220684
1
2871
2965
annotation2220677
1
BBa_K302017
range2220677
1
98
2870
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K302017_sequence
1
aaaggtggtgaatactagatgttgtttttgaaaagaagtgttaacattttaggctgttttattgttttatttatgttatctgcttgtcaatcattaacaaagtttaaagataaagttgttgaagaaacaaaaaaaacaatgcattttactgatgataacgaaaacgatacatcggaaacaatggagagcctgattgataagggaaaacttgatcaagtggtctatgacgatcaactttatcatctgaaagagaaagttgatgaagacaaaaaaggaaaagtaataggggcaataggccaaactttctttgtagatggtgatggaaagagatggtcggaagaagaattgaaagaaccgtatatctctaataacccggatgaaattagagagaaaaaaccattacgctatgggaaggtttatagtacaaacgaagattctgatgccaaagatgaaatcattgttgagtttaatcgtgagtattatcgagcggttttaataaagaatgaaaaggaataagggagagtgtgacatgagtacagtcttattagaaaccaaaagtcttacaaaaaagtttggcaaagagacagctgttgcagatgtttcattaactgttcaaaaaaacagcatttatggactgttgggaccaaatggagctggaaaatcgacaacgcttaaaattttaacaggaattttacggaaaacttcaggtgaaattctctttgatggacataaatggaaaagatctgatctgcaaactatcggatcgttaatagaagctccgcctctttacgataatttaactgcatttgaaaatgtgaaagtacatgccacgctgctcggtctttccgaagatcgcatacacagtgtgctaaagactgtggatatgcagcatgctggaaagaaaagagcaggccaattttcgatggggatgaggcagcggcttggaattgcaatcgccttattaaaccatcctaaactattaattcttgatgaacctacaaacgggttagatccaataggtatacaagagctcagagaccttattcgctcatttcctcaggaaggtataacggtgattttatctagtcacatcctttcggaagtcgagcaaatagcagatcatattgggattatcatcgacggtcatcttgggtaccagggtgaaataaacaaggctgatgatttggagcacttgtttatggaagttgtaaagtcaactcgtgaaaaagaaggtgtaaaaagatgataaattatataaaagccgaaaacctaaagtttaagaggacgttttccagaaaaatgattatctttgtagctcttcttaatataggtttttcttttttaatgaacactcaattttttgtaccaggaacttataactggtggtctattatatttatgccagtgatgattgctttattttgttctttatctcatcaaaaagaaaaaaaggcctctcattataacggtacgtattcattgcctatagatctagggaaattatggtacgctaagattattattattgcaatttattcactattatctcaggttgtatttttagtttttatgttactcatgggattcgttatagctgatttttccatcatttcgccttctatagttgccgcgagcttattgctttggttgacaagtctttggcaaattccgctctgtttatttgtggcaaaaaagtggggatttacaatggcggtaatgttaaatttcatagggacgctagttctcggtgttatgccggcatctcgtgccttttggtggttcattccttggagctggccaattcgaatgatgtgtccaaccattggtatacatcctaacggtttgctgctcgaaaacaatgatcctttgttgagttggatggttgtgcctcctgccatagtgatttcaattttattttttttgatactggcatttctgaccagccgttcgtttgccccaacaactgattacaaaatgaataagcgggaggcgatgtaaggtgaaattgcttttgaggtgtttacaagctgatttccagaagagcaagcacacctcttttttgtggctgcatcttatcattcctatcgtttgttcagttatattgattttgtatttttacggaagagatcaagtggaatttcacctttataagtcttttatggaagctatcgcagttgcattaccgctgctcatcggggtgctatgtggaatgactgcatccttagaagaacaggcagggaaatttcatgtattactagggagcacatctccgaaaatcgttgcttatgtgagtaaagtgttcatgctgcttttcttggagatttgctctattggtttagcactattcatctatttcattggattaaagtttattttaaaagtgccggatttatcttacgatatttttataatgggtggtgcctggctggttgcaggcagtgtagtcctgtattgtatttacttttttatcagttgtatgtttggaatgggggcttctgtcctgataggaggagcaggcctgctcatgacagctctcatgaacactggattaggggatgctatatggaaatttaacccgtgggcctgggggattagattgtcaagtttaaatgaagtacttcactttaaagaagtaaatcaagggcatcatcctttattaatgcatgaaataaattcaggggtattgatgatggtttttggtataatatgtacaatcattgccagtcttatatggtttagtaaatgggaaggcagaaaaacgtttgaataataa
BBa_K143012_sequence
1
aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt
BBa_K302018_sequence
1
aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtaaaggtggtgaatactagatgttgtttttgaaaagaagtgttaacattttaggctgttttattgttttatttatgttatctgcttgtcaatcattaacaaagtttaaagataaagttgttgaagaaacaaaaaaaacaatgcattttactgatgataacgaaaacgatacatcggaaacaatggagagcctgattgataagggaaaacttgatcaagtggtctatgacgatcaactttatcatctgaaagagaaagttgatgaagacaaaaaaggaaaagtaataggggcaataggccaaactttctttgtagatggtgatggaaagagatggtcggaagaagaattgaaagaaccgtatatctctaataacccggatgaaattagagagaaaaaaccattacgctatgggaaggtttatagtacaaacgaagattctgatgccaaagatgaaatcattgttgagtttaatcgtgagtattatcgagcggttttaataaagaatgaaaaggaataagggagagtgtgacatgagtacagtcttattagaaaccaaaagtcttacaaaaaagtttggcaaagagacagctgttgcagatgtttcattaactgttcaaaaaaacagcatttatggactgttgggaccaaatggagctggaaaatcgacaacgcttaaaattttaacaggaattttacggaaaacttcaggtgaaattctctttgatggacataaatggaaaagatctgatctgcaaactatcggatcgttaatagaagctccgcctctttacgataatttaactgcatttgaaaatgtgaaagtacatgccacgctgctcggtctttccgaagatcgcatacacagtgtgctaaagactgtggatatgcagcatgctggaaagaaaagagcaggccaattttcgatggggatgaggcagcggcttggaattgcaatcgccttattaaaccatcctaaactattaattcttgatgaacctacaaacgggttagatccaataggtatacaagagctcagagaccttattcgctcatttcctcaggaaggtataacggtgattttatctagtcacatcctttcggaagtcgagcaaatagcagatcatattgggattatcatcgacggtcatcttgggtaccagggtgaaataaacaaggctgatgatttggagcacttgtttatggaagttgtaaagtcaactcgtgaaaaagaaggtgtaaaaagatgataaattatataaaagccgaaaacctaaagtttaagaggacgttttccagaaaaatgattatctttgtagctcttcttaatataggtttttcttttttaatgaacactcaattttttgtaccaggaacttataactggtggtctattatatttatgccagtgatgattgctttattttgttctttatctcatcaaaaagaaaaaaaggcctctcattataacggtacgtattcattgcctatagatctagggaaattatggtacgctaagattattattattgcaatttattcactattatctcaggttgtatttttagtttttatgttactcatgggattcgttatagctgatttttccatcatttcgccttctatagttgccgcgagcttattgctttggttgacaagtctttggcaaattccgctctgtttatttgtggcaaaaaagtggggatttacaatggcggtaatgttaaatttcatagggacgctagttctcggtgttatgccggcatctcgtgccttttggtggttcattccttggagctggccaattcgaatgatgtgtccaaccattggtatacatcctaacggtttgctgctcgaaaacaatgatcctttgttgagttggatggttgtgcctcctgccatagtgatttcaattttattttttttgatactggcatttctgaccagccgttcgtttgccccaacaactgattacaaaatgaataagcgggaggcgatgtaaggtgaaattgcttttgaggtgtttacaagctgatttccagaagagcaagcacacctcttttttgtggctgcatcttatcattcctatcgtttgttcagttatattgattttgtatttttacggaagagatcaagtggaatttcacctttataagtcttttatggaagctatcgcagttgcattaccgctgctcatcggggtgctatgtggaatgactgcatccttagaagaacaggcagggaaatttcatgtattactagggagcacatctccgaaaatcgttgcttatgtgagtaaagtgttcatgctgcttttcttggagatttgctctattggtttagcactattcatctatttcattggattaaagtttattttaaaagtgccggatttatcttacgatatttttataatgggtggtgcctggctggttgcaggcagtgtagtcctgtattgtatttacttttttatcagttgtatgtttggaatgggggcttctgtcctgataggaggagcaggcctgctcatgacagctctcatgaacactggattaggggatgctatatggaaatttaacccgtgggcctgggggattagattgtcaagtttaaatgaagtacttcactttaaagaagtaaatcaagggcatcatcctttattaatgcatgaaataaattcaggggtattgatgatggtttttggtataatatgtacaatcattgccagtcttatatggtttagtaaatgggaaggcagaaaaacgtttgaataataatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z