BBa_K302018
type: ComponentDefinition
persistent identity: BBa K302018
display id: BBa_K302018
version: 1
was generated by: iGEM to SBOL conversion
description: Subtilin immunity
created: 2010-08-17T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: Gene Bank
owned by: james
owned by: myers
mutable description: Coding sequences for spaIFEG cluster. Device uses Pveg/spoVG promoter/RBS (BBa_K143053) and double terminator (BBa_B0014). This device will confer immunity to subtilin lantibiotic.
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 0
owner id: 6345
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: SpaI uses spoVG RBS (BBa_K143021), spaFEG uses native RBS (spaF and spaE RBS are in the coding sequence of preceding protein coding sequence). SpaG has had its TAG stop codon replaced with TAATAA.
star: false
creator: Steven Woodhouse, Alan Koh, Rachel May Boyd, Philip Hall
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_B0014
component: BBa_K302017
component: BBa_K143012
sequence annotation: BBa_K143012
sequence annotation: BBa_K302017
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K302018_sequence/1

BBa_B0014
type: Component
persistent identity: component2220684
display id: component2220684
version: 1
top level: BBa_K302018
definition: BBa_B0014
access: public

BBa_K302017
type: Component
persistent identity: component2220677
display id: component2220677
version: 1
top level: BBa_K302018
definition: BBa_K302017
access: public

BBa_K143012
type: Component
persistent identity: component2220664
display id: component2220664
version: 1
top level: BBa_K302018
definition: Pveg
access: public

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation2220664
display id: annotation2220664
version: 1
top level: BBa_K302018
location: https://synbiohub.org/public/igem/BBa_K302018/annotation2220664/range2220664/1
component: BBa_K143012

https://synbiohub.org/public/igem/BBa_K302018/annotation2220664/range2220664/1
type: Range
persistent identity: range2220664
display id: range2220664
version: 1
top level: BBa_K302018
direction: direction/reverse
start: 1
end: 97
orientation: inline

BBa_K302017
type: SequenceAnnotation
persistent identity: annotation2220677
display id: annotation2220677
version: 1
top level: BBa_K302018
location: https://synbiohub.org/public/igem/BBa_K302018/annotation2220677/range2220677/1
component: BBa_K302017

https://synbiohub.org/public/igem/BBa_K302018/annotation2220677/range2220677/1
type: Range
persistent identity: range2220677
display id: range2220677
version: 1
top level: BBa_K302018
direction: direction/reverse
start: 98
end: 2870
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2220684
display id: annotation2220684
version: 1
top level: BBa_K302018
location: https://synbiohub.org/public/igem/BBa_K302018/annotation2220684/range2220684/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K302018/annotation2220684/range2220684/1
type: Range
persistent identity: range2220684
display id: range2220684
version: 1
top level: BBa_K302018
direction: direction/reverse
start: 2871
end: 2965
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

Pveg
type: ComponentDefinition
persistent identity: BBa K143012
display id: BBa_K143012
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter veg a constitutive promoter for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor#1. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
star: false
creator: James Chappell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143012_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation1975704
display id: annotation1975704
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
type: Range
persistent identity: range1975704
display id: range1975704
version: 1
top level: Pveg
direction: direction/forward
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975705
display id: annotation1975705
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
type: Range
persistent identity: range1975705
display id: range1975705
version: 1
top level: Pveg
direction: direction/forward
start: 86
end: 91
orientation: inline

BBa_K302017
type: ComponentDefinition
persistent identity: BBa K302017
display id: BBa_K302017
version: 1
was generated by: iGEM to SBOL conversion
description: spaIFEG cluster
created: 2010-08-17T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: Gene bank
owned by: james
owned by: myers
mutable description: Coding sequences and RBS' for spaIFEG cluster.
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 0
owner id: 6345
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: SpaI uses spoVG RBS (BBa_K143021), spaFEG uses native RBS (spaF and spaE RBS are in the coding sequence of preceding protein coding sequence). SpaG has had its TAG stop codon replaced with TAATAA.
star: false
creator: Steven Woodhouse, Alan Koh, Rachel May Boyd, Philip Hall
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: spoVG
sequence annotation: BBa_K143021
sequence annotation: BBa_G0000
sequence annotation: BioBrick assembly scar
sequence annotation: spaI
sequence annotation: BBa_K302006
sequence annotation: spaF
sequence annotation: BBa_K302007
sequence annotation: spaE
sequence annotation: BBa_K302008
sequence annotation: BBa_K302009
sequence annotation: spaG
sequence: https://synbiohub.org/public/igem/BBa_K302017_sequence/1

spoVG
type: SequenceAnnotation
persistent identity: annotation2078327
display id: annotation2078327
version: 1
top level: BBa_K302017
location: https://synbiohub.org/public/igem/BBa_K302017/annotation2078327/range2078327/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K302017/annotation2078327/range2078327/1
type: Range
persistent identity: range2078327
display id: range2078327
version: 1
top level: BBa_K302017
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2078326
display id: annotation2078326
version: 1
top level: BBa_K302017
location: https://synbiohub.org/public/igem/BBa_K302017/annotation2078326/range2078326/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K302017/annotation2078326/range2078326/1
type: Range
persistent identity: range2078326
display id: range2078326
version: 1
top level: BBa_K302017
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2078328
display id: annotation2078328
version: 1
top level: BBa_K302017
location: https://synbiohub.org/public/igem/BBa_K302017/annotation2078328/range2078328/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K302017/annotation2078328/range2078328/1
type: Range
persistent identity: range2078328
display id: range2078328
version: 1
top level: BBa_K302017
direction: direction/reverse
start: 13
end: 18
orientation: inline

BioBrick assembly scar
type: SequenceAnnotation
persistent identity: annotation2078329
display id: annotation2078329
version: 1
top level: BBa_K302017
location: https://synbiohub.org/public/igem/BBa_K302017/annotation2078329/range2078329/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K302017/annotation2078329/range2078329/1
type: Range
persistent identity: range2078329
display id: range2078329
version: 1
top level: BBa_K302017
direction: direction/forward
start: 13
end: 18
orientation: inline

spaI
type: SequenceAnnotation
persistent identity: annotation2078331
display id: annotation2078331
version: 1
top level: BBa_K302017
location: https://synbiohub.org/public/igem/BBa_K302017/annotation2078331/range2078331/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K302017/annotation2078331/range2078331/1
type: Range
persistent identity: range2078331
display id: range2078331
version: 1
top level: BBa_K302017
direction: direction/forward
start: 19
end: 516
orientation: inline

BBa_K302006
type: SequenceAnnotation
persistent identity: annotation2078330
display id: annotation2078330
version: 1
top level: BBa_K302017
location: https://synbiohub.org/public/igem/BBa_K302017/annotation2078330/range2078330/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K302017/annotation2078330/range2078330/1
type: Range
persistent identity: range2078330
display id: range2078330
version: 1
top level: BBa_K302017
direction: direction/reverse
start: 19
end: 516
orientation: inline

spaF
type: SequenceAnnotation
persistent identity: annotation2078333
display id: annotation2078333
version: 1
top level: BBa_K302017
location: https://synbiohub.org/public/igem/BBa_K302017/annotation2078333/range2078333/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K302017/annotation2078333/range2078333/1
type: Range
persistent identity: range2078333
display id: range2078333
version: 1
top level: BBa_K302017
direction: direction/forward
start: 503
end: 1246
orientation: inline

BBa_K302007
type: SequenceAnnotation
persistent identity: annotation2078332
display id: annotation2078332
version: 1
top level: BBa_K302017
location: https://synbiohub.org/public/igem/BBa_K302017/annotation2078332/range2078332/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K302017/annotation2078332/range2078332/1
type: Range
persistent identity: range2078332
display id: range2078332
version: 1
top level: BBa_K302017
direction: direction/reverse
start: 503
end: 1246
orientation: inline

spaE
type: SequenceAnnotation
persistent identity: annotation2078335
display id: annotation2078335
version: 1
top level: BBa_K302017
location: https://synbiohub.org/public/igem/BBa_K302017/annotation2078335/range2078335/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K302017/annotation2078335/range2078335/1
type: Range
persistent identity: range2078335
display id: range2078335
version: 1
top level: BBa_K302017
direction: direction/forward
start: 1243
end: 1997
orientation: inline

BBa_K302008
type: SequenceAnnotation
persistent identity: annotation2078334
display id: annotation2078334
version: 1
top level: BBa_K302017
location: https://synbiohub.org/public/igem/BBa_K302017/annotation2078334/range2078334/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K302017/annotation2078334/range2078334/1
type: Range
persistent identity: range2078334
display id: range2078334
version: 1
top level: BBa_K302017
direction: direction/reverse
start: 1243
end: 1997
orientation: inline

BBa_K302009
type: SequenceAnnotation
persistent identity: annotation2078336
display id: annotation2078336
version: 1
top level: BBa_K302017
location: https://synbiohub.org/public/igem/BBa_K302017/annotation2078336/range2078336/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K302017/annotation2078336/range2078336/1
type: Range
persistent identity: range2078336
display id: range2078336
version: 1
top level: BBa_K302017
direction: direction/reverse
start: 2159
end: 2773
orientation: inline

spaG
type: SequenceAnnotation
persistent identity: annotation2078337
display id: annotation2078337
version: 1
top level: BBa_K302017
location: https://synbiohub.org/public/igem/BBa_K302017/annotation2078337/range2078337/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K302017/annotation2078337/range2078337/1
type: Range
persistent identity: range2078337
display id: range2078337
version: 1
top level: BBa_K302017
direction: direction/forward
start: 2159
end: 2773
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K302018_sequence/1
type: Sequence
persistent identity: BBa K302018 sequence
display id: BBa_K302018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtaaaggtggtgaatactagatgttgtttttgaaaagaagtgttaacattttaggctgttttattgttttatttatgttatctgcttgtcaatcattaacaaagtttaaagataaagttgttgaagaaacaaaaaaaacaatgcattttactgatgataacgaaaacgatacatcggaaacaatggagagcctgattgataagggaaaacttgatcaagtggtctatgacgatcaactttatcatctgaaagagaaagttgatgaagacaaaaaaggaaaagtaataggggcaataggccaaactttctttgtagatggtgatggaaagagatggtcggaagaagaattgaaagaaccgtatatctctaataacccggatgaaattagagagaaaaaaccattacgctatgggaaggtttatagtacaaacgaagattctgatgccaaagatgaaatcattgttgagtttaatcgtgagtattatcgagcggttttaataaagaatgaaaaggaataagggagagtgtgacatgagtacagtcttattagaaaccaaaagtcttacaaaaaagtttggcaaagagacagctgttgcagatgtttcattaactgttcaaaaaaacagcatttatggactgttgggaccaaatggagctggaaaatcgacaacgcttaaaattttaacaggaattttacggaaaacttcaggtgaaattctctttgatggacataaatggaaaagatctgatctgcaaactatcggatcgttaatagaagctccgcctctttacgataatttaactgcatttgaaaatgtgaaagtacatgccacgctgctcggtctttccgaagatcgcatacacagtgtgctaaagactgtggatatgcagcatgctggaaagaaaagagcaggccaattttcgatggggatgaggcagcggcttggaattgcaatcgccttattaaaccatcctaaactattaattcttgatgaacctacaaacgggttagatccaataggtatacaagagctcagagaccttattcgctcatttcctcaggaaggtataacggtgattttatctagtcacatcctttcggaagtcgagcaaatagcagatcatattgggattatcatcgacggtcatcttgggtaccagggtgaaataaacaaggctgatgatttggagcacttgtttatggaagttgtaaagtcaactcgtgaaaaagaaggtgtaaaaagatgataaattatataaaagccgaaaacctaaagtttaagaggacgttttccagaaaaatgattatctttgtagctcttcttaatataggtttttcttttttaatgaacactcaattttttgtaccaggaacttataactggtggtctattatatttatgccagtgatgattgctttattttgttctttatctcatcaaaaagaaaaaaaggcctctcattataacggtacgtattcattgcctatagatctagggaaattatggtacgctaagattattattattgcaatttattcactattatctcaggttgtatttttagtttttatgttactcatgggattcgttatagctgatttttccatcatttcgccttctatagttgccgcgagcttattgctttggttgacaagtctttggcaaattccgctctgtttatttgtggcaaaaaagtggggatttacaatggcggtaatgttaaatttcatagggacgctagttctcggtgttatgccggcatctcgtgccttttggtggttcattccttggagctggccaattcgaatgatgtgtccaaccattggtatacatcctaacggtttgctgctcgaaaacaatgatcctttgttgagttggatggttgtgcctcctgccatagtga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143012_sequence/1
type: Sequence
persistent identity: BBa K143012 sequence
display id: BBa_K143012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302017_sequence/1
type: Sequence
persistent identity: BBa K302017 sequence
display id: BBa_K302017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggtggtgaatactagatgttgtttttgaaaagaagtgttaacattttaggctgttttattgttttatttatgttatctgcttgtcaatcattaacaaagtttaaagataaagttgttgaagaaacaaaaaaaacaatgcattttactgatgataacgaaaacgatacatcggaaacaatggagagcctgattgataagggaaaacttgatcaagtggtctatgacgatcaactttatcatctgaaagagaaagttgatgaagacaaaaaaggaaaagtaataggggcaataggccaaactttctttgtagatggtgatggaaagagatggtcggaagaagaattgaaagaaccgtatatctctaataacccggatgaaattagagagaaaaaaccattacgctatgggaaggtttatagtacaaacgaagattctgatgccaaagatgaaatcattgttgagtttaatcgtgagtattatcgagcggttttaataaagaatgaaaaggaataagggagagtgtgacatgagtacagtcttattagaaaccaaaagtcttacaaaaaagtttggcaaagagacagctgttgcagatgtttcattaactgttcaaaaaaacagcatttatggactgttgggaccaaatggagctggaaaatcgacaacgcttaaaattttaacaggaattttacggaaaacttcaggtgaaattctctttgatggacataaatggaaaagatctgatctgcaaactatcggatcgttaatagaagctccgcctctttacgataatttaactgcatttgaaaatgtgaaagtacatgccacgctgctcggtctttccgaagatcgcatacacagtgtgctaaagactgtggatatgcagcatgctggaaagaaaagagcaggccaattttcgatggggatgaggcagcggcttggaattgcaatcgccttattaaaccatcctaaactattaattcttgatgaacctacaaacgggttagatccaataggtatacaagagctcagagaccttattcgctcatttcctcaggaaggtataacggtgattttatctagtcacatcctttcggaagtcgagcaaatagcagatcatattgggattatcatcgacggtcatcttgggtaccagggtgaaataaacaaggctgatgatttggagcacttgtttatggaagttgtaaagtcaactcgtgaaaaagaaggtgtaaaaagatgataaattatataaaagccgaaaacctaaagtttaagaggacgttttccagaaaaatgattatctttgtagctcttcttaatataggtttttcttttttaatgaacactcaattttttgtaccaggaacttataactggtggtctattatatttatgccagtgatgattgctttattttgttctttatctcatcaaaaagaaaaaaaggcctctcattataacggtacgtattcattgcctatagatctagggaaattatggtacgctaagattattattattgcaatttattcactattatctcaggttgtatttttagtttttatgttactcatgggattcgttatagctgatttttccatcatttcgccttctatagttgccgcgagcttattgctttggttgacaagtctttggcaaattccgctctgtttatttgtggcaaaaaagtggggatttacaatggcggtaatgttaaatttcatagggacgctagttctcggtgttatgccggcatctcgtgccttttggtggttcattccttggagctggccaattcgaatgatgtgtccaaccattggtatacatcctaacggtttgctgctcgaaaacaatgatcctttgttgagttggatggttgtgcctcctgccatagtgatttcaattttattttttttgatactggcatttctgaccagccgttcgtttgccccaacaactgattacaaaatgaataagcgggaggcgatgtaagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z