LOCUS BBa_K302021 6683 bp DNA linear 26-Dec-2024 DEFINITION Oxygen-limitation induced subtilin production ACCESSION BBa_K302021 VERSION BBa_K302021.1 FEATURES Location/Qualifiers promoter 1..81 /label=BBa_K302020 RBS 82..93 /label=rbs RBS 82..93 /label=BBa_K143021 misc_feature 94..99 /label=BBa_G0000 misc_feature 177..177 /label=restriction site removed misc_feature 1242..1242 /label=restriction site removed misc_feature 2019..2019 /label=restriction site removed misc_feature 2673..2673 /label=restriction site removed misc_feature 2991..2991 /label=restriction site removed CDS 100..3192 /label=BBa_K302028 RBS 3193..3204 /label=rbs RBS 3193..3204 /label=BBa_K143021 misc_feature 3205..3210 /label=BBa_G0000 misc_feature 3804..3804 /label=restriction site removed CDS 3211..5055 /label=BBa_K302027 RBS 5056..5067 /label=rbs RBS 5056..5067 /label=BBa_K143021 misc_feature 5068..5073 /label=BBa_G0000 CDS 5074..6399 /label=BBa_K302026 RBS 6400..6411 /label=rbs RBS 6400..6411 /label=BBa_K143021 misc_feature 6412..6417 /label=BBa_G0000 CDS 6418..6588 /label=BBa_K302023 stem_loop 6596..6615 /label=T7 TE misc_feature 6622..6622 /label=stop polyA_site 6616..6629 /label=polya misc_feature 6589..6629 /label=BBa_B0012 terminator 6589..6629 /label=BBa_B0012 stem_loop 6650..6672 /label=stem_loop misc_feature 6638..6683 /label=BBa_B0011 terminator 6638..6683 /label=BBa_B0011 terminator 6589..6683 /label=BBa_B0014 ORIGIN 1 cactggggca ttcacaagat tgttagtttt ttctcgatgg gtacaaatca attcacgtta 61 caataaacac acaacgtgac taaaggtggt gaatactaga tgaaatcctt atatacacct 121 acagattatt atatgattcg ggttccttta gtacatcaag acttaaaaaa tgagaactct 181 caggatatcg atcagttatt acatgacctt tgcaacgatt cattatttcg ggaacaaata 241 ctggtatcta gcaggacact atatgaaaca atacatactt tcctgcaagc gccggataaa 301 ttaaaaggga aaaaaaagcg caactttcaa caggctattt tgaagtatgc aacaagaaga 361 gcaacaagaa ccacaccttt tggccttttt tcttcagtcg gtatagggtc gttttctgat 421 aaaaatcact tatcttttaa tcaacattca ttttacaaaa aggctcgtgt tgatttggag 481 tggctctacc aattaattag aaaattggaa aacgaataca ccgaccgact ttcttttaca 541 ttaaattccg cttgttatat taagggtgac cgggcttact tgttgtacag cacagatgga 601 aaatctgaag aagttagtgt tcgtgcgaca tctgttttct atttgataaa tgaactgtgt 661 ggtgaatctg ctgcatatca agatataatc cgttgtttga tagataacta tccaaatact 721 ccaataaata aaattaatca gtacgtagca gaccttattg acaaagagtt ccttatatca 781 aacttacggc cgccgatgac tgtttcagat caatttcagt atttaattga tcaagcggaa 841 agccgccata ttccaaatga actcattcag gcttgtaaag acattcaata tcagatagat 901 gcatataatc ggatcactat cggagaggga gagcatcagt atttaaatct gattgaaaca 961 atgaataaac tcataaaggc atcatctcct ctgcaagtag acgctgggct ggcagactcc 1021 tccattcaat tagataatga aacatctctc gccataagtg aattggcaag catgtttact 1081 tatatggctt ctccctctgc caatacatta gaccacttgg aaaaatacca caatgtattt 1141 ttggaacgct atggatatga aagagaagtt cctctcttag aaatgctatg ttccagcact 1201 ggcattggtg ctcctgctac gtacacgaat cctgctaatg agttctttga agaaacatca 1261 tttggggagc aattttcacc ggaaatgaaa caatttttca tgagaaagta ttttgaatca 1321 gttagaaaga aagctcctat tcaattagac gacgaaacat tccatagaat ttgcaactct 1381 gaaattgctg atgaggaaat tccattatca tttgaactca atttctttgt taaattacga 1441 aatgggagag ttaagcttta tttaggcccc aacgtcggat ctacccgcgc agggaaaaca 1501 tttgggagat tttctcatat gtctgattca atcagtgaaa tcataaagac cttacataac 1561 aaggagaaag agttaacaga gtgtaatacg aaagtttgtg aactgagtat tgtgcctaat 1621 caaactaggt ctggaaatgt aacaagaaat gtaagctacc gagaaaaaga gatgtctctt 1681 tttacgaaca gtgctctgca tctcaatgat tccgtcaaag ctgaagatat tctaattgga 1741 atcaataaag accataactt ttatgctaga cataaaacaa ctggcgaaat tctgtctttt 1801 gagtcaaatc atatgtttaa tcctttatta atgaccaacg ctgtacggtt tttattagag 1861 atttcaagag atggtaaaag aaaatggaat gatttcccgt ggtttagtat ctatagtgat 1921 ttcaagtata ttcctgaaat caaatataaa gagataacct tatcttgtga acaatggctg 1981 atatacaaaa atgatttaag catgcactcg aatgcatcac tagaagagat aaaatctcct 2041 ttttttgaat ttcatcgtac ttatgaactg ccgcaaacat tttatatcgt taacgcagac 2101 aatcgattat tgattgatat agagaatgat tgtactttgg atgttttttt ctgggaattg 2161 aaaaaaacga accataacca gccattacaa cttgtggctg ttgagcatga tgcagatgcg 2221 ttaatggata gaaaccaaaa tgactattcg ggagaaatcg tcgttccgct gcttagaaaa 2281 caaccagaaa aaccattgta tttaccggtt cttaacgcaa tagagggaag tggttccgac 2341 agaataaaaa tgccatttga agactggctg tttattaagc tttattgtaa acaaacaaga 2401 gaagaagagc taattgcttt tgaaatagcg gatttttata accagatttc tgatcaatat 2461 ccagtcagac atttctttat gaggtatcgg gatccaaagc ctcatataag acttagattt 2521 aatggaaaag ccgaagtgct gtacagcttg tttccccaat tattgaattg gctgaaaagc 2581 ttaagagaaa aaggactggt ttcagagtct gttatcactc aatacgagcg ggagatagaa 2641 cgatatggcg ggctaagcct tatggaggct gcggaacagc ttttctgtga agacagcaaa 2701 gttgttgaaa tgatgattag aatgcaccgg atgaaagata ttacgataag caaggaaatt 2761 gcaggcatgg tttcggttat acagttttta gaacagttcg agctaacgtt tgaagaacag 2821 ttaacttttt tagagagaaa ttccttacag aatgagtatc gtactgaatt taaaaaggat 2881 agagaaatgt atattgaaat atgcaattct gacagagatt gggataatct caagaaaaca 2941 agtgatggcg gtatgttata tgaaactttg aaaacaagaa aaatggctgc ggctcattat 3001 gcatttttaa tcaaaaaggc atttgataac aaagatgaag tttattcacg tataggaagt 3061 atcatccatc tgcattgcaa tcgtttattc ggaaccgaca gagaactgga aaataaaatt 3121 ctcaccctat gcagacattc tttatatgcg caacgatatc aaaagatgaa tggtagttta 3181 gcatggaagt aaaaaggtgg tgaatactag atggaagtaa aggaacaact gaaactaaaa 3241 gagctgctgt ttatcatgaa acaaatgcct aagacgttca agttgatttt caccttagaa 3301 agatcactgt ttttaaagtt gattcgattc agtattatta ccggtattct gccaattgtt 3361 tcactatata tttcacaaga actgattaat tcccttgtga ctatccggaa agaggtttca 3421 attgttatca ccattttctt gacatatcta ggggtatctt ttttttcgga gctcatttcg 3481 cagatttccg aattttataa tggtaaattt caattaaata ttggttataa actcaactat 3541 aaagtcatga aaaagagcag taatttagct ctcaaggatt ttgaaaatcc agaaatatat 3601 gacaagttag agagggtaac gaaagagatc agttataagc cctatcagat tattcaagcc 3661 atcattacaa tgaccacatc atttgtgacg ctactctcat caattgcgtt tttaatgtcg 3721 tggaacccta aagtctcact tctgttgtta gttattccgg taatttctct attctatttc 3781 ttgaaaattg gacaggaaga atttttcata cactggaaac gcgcgggaaa ggaaaggaaa 3841 tcttggtata tcagctatat actcacacat gatttttctt ttaaagaatt aaaattgtat 3901 aaccttaagg attatttatt aaacaagtac tgggatatca aaaaatcatt tatagaacaa 3961 gatacaaaga tattaagaaa gaaaacttta ttgaacctaa tatatgagat tgcagtgcaa 4021 ttagtcggag ctgtcattat atttattgcc atcatgtctg cttttgcggg aaaaataatg 4081 gtcggtaatg taatgagtta tatcagatcg gtttcgctgg tgcaaaatca ctcacaatct 4141 atcatgacaa gcatttattc gatctataac agcaatctct atatgaatca attgtatgaa 4201 tttctggaat taaaagaaga gaaaagtcaa ggtcacaaga agccgattgt tgagcctatt 4261 cattctgttg tttttcaaaa tgtcagcttt atctatccca atcaaggaga acagacactg 4321 aaacatatta acgtttcatt gcataaagga gaacgtgtag ccattgttgg accaaacgga 4381 tcagggaaaa aaacattcat taagctcctt accggcttat atgaagttca cgagggcgac 4441 attttgatta atggaataaa tattaaagaa ctggatatgg acagttatat gaatcaaatt 4501 gcagcgctat ttcaagactt tatgaaatac gaaatgacat taaaagaaaa tataggattc 4561 ggccaaattg ataaactaca tcaaacaaac aaaatgcatg aagttctcga tattgtgaga 4621 gccgatttct taaaaagcca ctcttcctat caattcgata cacagctcgg actatggttt 4681 gatgaaggaa gacagctatc cggtggacaa tggcaaaaga ttgctttggc aagggcttat 4741 tttagggaag cttctttgta tattttggac gagccaagtt ctgcgctgga tccaattgca 4801 gaaaaagaaa ccttcgatac ttttttcagt ttgtcaaaag ataaaatagg aatatttatt 4861 tctcatcgat tggttgctgc taaacttgca gatcggatca ttgttatgga taaaggagag 4921 attgtaggaa taggcacaca tgaagagcta ttaaaaacat gtccgctgta taaaaaaatg 4981 gatgaatccg agaactatat gaacccactg gaggaggaag gcagtaaatg gaaagaggca 5041 ctgtatcaag gatagaaagg tggtgaatac tagatggaaa gaggcactgt atcaaggata 5101 gaagttgaaa ttgtgaaaga aatggctcgt caaatttcta attacgacaa agtgcttgaa 5161 atagtgaatc aaaaagataa ctttcgcagc attggcgaag ttccgcttat accttggaaa 5221 tcaactgctt tgagtcatgg tataccaggc atttgtatgc tgtatggcga attgcacgct 5281 cattttcctg aggaaggatg ggacgacata ggacatcaat atttatcgat tttagtaaat 5341 gaaataaaag aaaaagggct gcatactcct tcaatgtttt ctggagcagc tggcattgga 5401 ctggcagcaa tctgtttatc gcaacgtttc acctattaca atggtttgat ttctgatatt 5461 aatgaatatt tggctgaaac cgtacctcaa ttgttaactg aatttgatca gcggcaagtg 5521 tgtatgagtg actacgatgt catagaagga gttagcggaa ttgccaacta tctgctgtta 5581 tttcaagaag ataaagctat gggagatttg ttaattgata ttttgaaata tctcgttagg 5641 ttaactgaag acattatcgt ggatggagaa aaagttcctg gatggcatat tccatctcaa 5701 catcagttta ctgatattga aaaaaaagct tatccttacg gcaattttaa tatgggatta 5761 gcacatggga tacctggtcc tatttgtgtt ctctcttcag cactcataca gggaattaaa 5821 gtaaagggac aagaagctgc gattgaaaaa atggcgaatt tcctactgga attttctgaa 5881 aaagagcaag acagcttgtt ttggaaagga atcataagct ttgaggagta tcaatacgga 5941 tctccgccaa acgcagtaaa ttttagcaga gatgcttggt gttatggaag acctggtgta 6001 tgtctagcgc ttgttaaagc cggaaaagca cttcaaaata ctgaacttat aaatatcgga 6061 gtacagaatt taagatacac aatatctgat atacggggaa ttttttcacc taccatatgt 6121 catggttaca gcggtatcgg tcagattctg cttgctgtaa atctactcac cggacaggag 6181 tattttaagg aagagcttca agagattaaa caaaagatca tgagctacta tgacaaggat 6241 tatatctttg gattccataa ctatgaatca atggaagggg aggaagcagt acctttgcag 6301 tacgttggtt tgttggatgg agctgtaggt gtaggcttag gggtattaaa catggaatta 6361 ggctcaaaaa cagattggac aaaagcatta ttaatttaaa aaggtggtga atactagatg 6421 tcaaagttcg atgatttcga tttggatgtt gtgaaagtct ctaaacaaga ctcaaaaatc 6481 actccgcaat ggaaaagtga atcactttgt acaccaggat gtgtaactgg tgcattgcaa 6541 acttgcttcc ttcaaacact aacttgtaac tgcaaaatct ctaaataatc acactggctc 6601 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta 6661 ttaatccggc ttttttatta ttt //