BBa_K302027 1 BBa_K302027 spaT 2010-10-03T11:00:00Z 2015-05-08T01:11:52Z . . false false _442_ 0 6009 9 Not in stock false . false Steven Woodhouse annotation2081984 1 restriction site removed range2081984 1 594 594 BBa_K302028 1 BBa_K302028 spaB 2010-10-03T11:00:00Z 2015-05-08T01:11:52Z . . false false _442_ 0 6009 9 Not in stock false . false Steven Woodhouse annotation2081979 1 restriction site removed range2081979 1 78 78 annotation2081980 1 restriction site removed range2081980 1 1143 1143 annotation2081983 1 restriction site removed range2081983 1 2892 2892 annotation2081981 1 restriction site removed range2081981 1 1920 1920 annotation2081982 1 restriction site removed range2081982 1 2574 2574 BBa_K143021 1 RBS-spoVG SpoVG ribosome binding site (RBS) for B. subtilis 2008-09-16T11:00:00Z 2015-05-08T01:10:23Z The sequence was taken from a previous research paper [1] and was constructed by Geneart. Released HQ 2013 Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal <cite>1</cite> false true _199_ 0 2090 9 In stock false In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained. false James Chappell annotation1975997 1 rbs range1975997 1 1 12 BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939303 1 BBa_B0012 component939311 1 BBa_B0011 annotation939311 1 BBa_B0011 range939311 1 50 95 annotation939303 1 BBa_B0012 range939303 1 1 41 BBa_K302021 1 BBa_K302021 Oxygen-limitation induced subtilin production 2010-09-24T11:00:00Z 2015-05-08T01:11:52Z .. . false false _442_ 0 6009 9 Not in stock true . false Alan Koh, Philip Hall, Steven Woodhouse, Rachel May Boyd component2235379 1 BBa_K302028 component2235391 1 BBa_G0000 component2235382 1 BBa_G0000 component2235399 1 BBa_B0014 component2235384 1 BBa_K302027 component2235373 1 BBa_G0000 component2235386 1 BBa_K143021 component2235381 1 BBa_K143021 component2235390 1 BBa_K143021 component2235372 1 BBa_K143021 component2235370 1 BBa_K302020 component2235392 1 BBa_K302023 component2235388 1 BBa_K302026 component2235387 1 BBa_G0000 annotation2235370 1 BBa_K302020 range2235370 1 1 81 annotation2235387 1 BBa_G0000 range2235387 1 5068 5073 annotation2235388 1 BBa_K302026 range2235388 1 5074 6399 annotation2235392 1 BBa_K302023 range2235392 1 6418 6588 annotation2235391 1 BBa_G0000 range2235391 1 6412 6417 annotation2235386 1 BBa_K143021 range2235386 1 5056 5067 annotation2235384 1 BBa_K302027 range2235384 1 3211 5055 annotation2235372 1 BBa_K143021 range2235372 1 82 93 annotation2235390 1 BBa_K143021 range2235390 1 6400 6411 annotation2235382 1 BBa_G0000 range2235382 1 3205 3210 annotation2235381 1 BBa_K143021 range2235381 1 3193 3204 annotation2235373 1 BBa_G0000 range2235373 1 94 99 annotation2235399 1 BBa_B0014 range2235399 1 6589 6683 annotation2235379 1 BBa_K302028 range2235379 1 100 3192 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation1683 1 stem_loop range1683 1 13 35 annotation7019 1 BBa_B0011 range7019 1 1 46 BBa_K302023 1 BBa_K302023 spaS 2010-10-02T11:00:00Z 2015-05-08T01:11:52Z . . false false _442_ 0 6009 9 In stock false . false Steven Woodhouse BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_K302026 1 BBa_K302026 spaC 2010-10-03T11:00:00Z 2015-05-08T01:11:52Z . . false false _442_ 0 6009 9 Not in stock false . false Steven Woodhouse BBa_G0000 1 scar SpeI/XbaI scar for RBS-CDS junctions 2007-07-22T11:00:00Z 2015-08-31T04:07:27Z SpeI/XbaI scar This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly. false true _41_ 0 126 162 Not in stock false This is a shorter scar to ensure proper spacing between the RBS and CDS. false Reshma Shetty BBa_K302020 1 BBa_K302020 Oxygen-limitation activated promoter 2010-09-24T11:00:00Z 2015-05-08T01:11:52Z . . false false _442_ 0 6009 9 Not in stock true . false Philip Hall, Alan Koh, Steven Woodhouse BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K302023_sequence 1 atgtcaaagttcgatgatttcgatttggatgttgtgaaagtctctaaacaagactcaaaaatcactccgcaatggaaaagtgaatcactttgtacaccaggatgtgtaactggtgcattgcaaacttgcttccttcaaacactaacttgtaactgcaaaatctctaaataa BBa_K302026_sequence 1 atggaaagaggcactgtatcaaggatagaagttgaaattgtgaaagaaatggctcgtcaaatttctaattacgacaaagtgcttgaaatagtgaatcaaaaagataactttcgcagcattggcgaagttccgcttataccttggaaatcaactgctttgagtcatggtataccaggcatttgtatgctgtatggcgaattgcacgctcattttcctgaggaaggatgggacgacataggacatcaatatttatcgattttagtaaatgaaataaaagaaaaagggctgcatactccttcaatgttttctggagcagctggcattggactggcagcaatctgtttatcgcaacgtttcacctattacaatggtttgatttctgatattaatgaatatttggctgaaaccgtacctcaattgttaactgaatttgatcagcggcaagtgtgtatgagtgactacgatgtcatagaaggagttagcggaattgccaactatctgctgttatttcaagaagataaagctatgggagatttgttaattgatattttgaaatatctcgttaggttaactgaagacattatcgtggatggagaaaaagttcctggatggcatattccatctcaacatcagtttactgatattgaaaaaaaagcttatccttacggcaattttaatatgggattagcacatgggatacctggtcctatttgtgttctctcttcagcactcatacagggaattaaagtaaagggacaagaagctgcgattgaaaaaatggcgaatttcctactggaattttctgaaaaagagcaagacagcttgttttggaaaggaatcataagctttgaggagtatcaatacggatctccgccaaacgcagtaaattttagcagagatgcttggtgttatggaagacctggtgtatgtctagcgcttgttaaagccggaaaagcacttcaaaatactgaacttataaatatcggagtacagaatttaagatacacaatatctgatatacggggaattttttcacctaccatatgtcatggttacagcggtatcggtcagattctgcttgctgtaaatctactcaccggacaggagtattttaaggaagagcttcaagagattaaacaaaagatcatgagctactatgacaaggattatatctttggattccataactatgaatcaatggaaggggaggaagcagtacctttgcagtacgttggtttgttggatggagctgtaggtgtaggcttaggggtattaaacatggaattaggctcaaaaacagattggacaaaagcattattaatttaa BBa_K143021_sequence 1 aaaggtggtgaa BBa_K302020_sequence 1 cactggggcattcacaagattgttagttttttctcgatgggtacaaatcaattcacgttacaataaacacacaacgtgact BBa_G0000_sequence 1 tactag BBa_K302021_sequence 1 cactggggcattcacaagattgttagttttttctcgatgggtacaaatcaattcacgttacaataaacacacaacgtgactaaaggtggtgaatactagatgaaatccttatatacacctacagattattatatgattcgggttcctttagtacatcaagacttaaaaaatgagaactctcaggatatcgatcagttattacatgacctttgcaacgattcattatttcgggaacaaatactggtatctagcaggacactatatgaaacaatacatactttcctgcaagcgccggataaattaaaagggaaaaaaaagcgcaactttcaacaggctattttgaagtatgcaacaagaagagcaacaagaaccacaccttttggccttttttcttcagtcggtatagggtcgttttctgataaaaatcacttatcttttaatcaacattcattttacaaaaaggctcgtgttgatttggagtggctctaccaattaattagaaaattggaaaacgaatacaccgaccgactttcttttacattaaattccgcttgttatattaagggtgaccgggcttacttgttgtacagcacagatggaaaatctgaagaagttagtgttcgtgcgacatctgttttctatttgataaatgaactgtgtggtgaatctgctgcatatcaagatataatccgttgtttgatagataactatccaaatactccaataaataaaattaatcagtacgtagcagaccttattgacaaagagttccttatatcaaacttacggccgccgatgactgtttcagatcaatttcagtatttaattgatcaagcggaaagccgccatattccaaatgaactcattcaggcttgtaaagacattcaatatcagatagatgcatataatcggatcactatcggagagggagagcatcagtatttaaatctgattgaaacaatgaataaactcataaaggcatcatctcctctgcaagtagacgctgggctggcagactcctccattcaattagataatgaaacatctctcgccataagtgaattggcaagcatgtttacttatatggcttctccctctgccaatacattagaccacttggaaaaataccacaatgtatttttggaacgctatggatatgaaagagaagttcctctcttagaaatgctatgttccagcactggcattggtgctcctgctacgtacacgaatcctgctaatgagttctttgaagaaacatcatttggggagcaattttcaccggaaatgaaacaatttttcatgagaaagtattttgaatcagttagaaagaaagctcctattcaattagacgacgaaacattccatagaatttgcaactctgaaattgctgatgaggaaattccattatcatttgaactcaatttctttgttaaattacgaaatgggagagttaagctttatttaggccccaacgtcggatctacccgcgcagggaaaacatttgggagattttctcatatgtctgattcaatcagtgaaatcataaagaccttacataacaaggagaaagagttaacagagtgtaatacgaaagtttgtgaactgagtattgtgcctaatcaaactaggtctggaaatgtaacaagaaatgtaagctaccgagaaaaagagatgtctctttttacgaacagtgctctgcatctcaatgattccgtcaaagctgaagatattctaattggaatcaataaagaccataacttttatgctagacataaaacaactggcgaaattctgtcttttgagtcaaatcatatgtttaatcctttattaatgaccaacgctgtacggtttttattagagatttcaagagatggtaaaagaaaatggaatgatttcccgtggtttagtatctatagtgatttcaagtatattcctgaaatcaaatataaagagataaccttatcttgtgaacaatggctgatatacaaaaatgatttaagcatgcactcgaatgcatcactagaagagataaaatctcctttttttgaatttcatcgtacttatgaactgccgcaaacattttatatcgttaacgcagacaatcgattattgattgatatagagaatgattgtactttggatgtttttttctgggaattgaaaaaaacgaaccataaccagccattacaacttgtggctgttgagcatgatgcagatgcgttaatggatagaaaccaaaatgactattcgggagaaatcgtcgttccgctgcttagaaaacaaccagaaaaaccattgtatttaccggttcttaacgcaatagagggaagtggttccgacagaataaaaatgccatttgaagactggctgtttattaagctttattgtaaacaaacaagagaagaagagctaattgcttttgaaatagcggatttttataaccagatttctgatcaatatccagtcagacatttctttatgaggtatcgggatccaaagcctcatataagacttagatttaatggaaaagccgaagtgctgtacagcttgtttccccaattattgaattggctgaaaagcttaagagaaaaaggactggtttcagagtctgttatcactcaatacgagcgggagatagaacgatatggcgggctaagccttatggaggctgcggaacagcttttctgtgaagacagcaaagttgttgaaatgatgattagaatgcaccggatgaaagatattacgataagcaaggaaattgcaggcatggtttcggttatacagtttttagaacagttcgagctaacgtttgaagaacagttaacttttttagagagaaattccttacagaatgagtatcgtactgaatttaaaaaggatagagaaatgtatattgaaatatgcaattctgacagagattgggataatctcaagaaaacaagtgatggcggtatgttatatgaaactttgaaaacaagaaaaatggctgcggctcattatgcatttttaatcaaaaaggcatttgataacaaagatgaagtttattcacgtataggaagtatcatccatctgcattgcaatcgtttattcggaaccgacagagaactggaaaataaaattctcaccctatgcagacattctttatatgcgcaacgatatcaaaagatgaatggtagtttagcatggaagtaaaaaggtggtgaatactagatggaagtaaaggaacaactgaaactaaaagagctgctgtttatcatgaaacaaatgcctaagacgttcaagttgattttcaccttagaaagatcactgtttttaaagttgattcgattcagtattattaccggtattctgccaattgtttcactatatatttcacaagaactgattaattcccttgtgactatccggaaagaggtttcaattgttatcaccattttcttgacatatctaggggtatcttttttttcggagctcatttcgcagatttccgaattttataatggtaaatttcaattaaatattggttataaactcaactataaagtcatgaaaaagagcagtaatttagctctcaaggattttgaaaatccagaaatatatgacaagttagagagggtaacgaaagagatcagttataagccctatcagattattcaagccatcattacaatgaccacatcatttgtgacgctactctcatcaattgcgtttttaatgtcgtggaaccctaaagtctcacttctgttgttagttattccggtaatttctctattctatttcttgaaaattggacaggaagaatttttcatacactggaaacgcgcgggaaaggaaaggaaatcttggtatatcagctatatactcacacatgatttttcttttaaagaattaaaattgtataaccttaaggattatttattaaacaagtactgggatatcaaaaaatcatttatagaacaagatacaaagatattaagaaagaaaactttattgaacctaatatatgagattgcagtgcaattagtcggagctgtcattatatttattgccatcatgtctgcttttgcgggaaaaataatggtcggtaatgtaatgagttatatcagatcggtttcgctggtgcaaaatcactcacaatctatcatgacaagcatttattcgatctataacagcaatctctatatgaatcaattgtatgaatttctggaattaaaagaagagaaaagtcaaggtcacaagaagccgattgttgagcctattcattctgttgtttttcaaaatgtcagctttatctatcccaatcaaggagaacagacactgaaacatattaacgtttcattgcataaaggagaacgtgtagccattgttggaccaaacggatcagggaaaaaaacattcattaagctccttaccggcttatatgaagttcacgagggcgacattttgattaatggaataaatattaaagaactggatatggacagttatatgaatcaaattgcagcgctatttcaagactttatgaaatacgaaatgacattaaaagaaaatataggattcggccaaattgataaactacatcaaacaaacaaaatgcatgaagttctcgatattgtgagagccgatttcttaaaaagccactcttcctatcaattcgatacacagctcggactatggtttgatgaaggaagacagctatccggtggacaatggcaaaagattgctttggcaagggcttattttagggaagcttctttgtatattttggacgagccaagttctgcgctggatccaattgcagaaaaagaaaccttcgatacttttttcagtttgtcaaaagataaaataggaatatttatttctcatcgattggttgctgctaaacttgcagatcggatcattgttatggataaaggagagattgtaggaataggcacacatgaagagctattaaaaacatgtccgctgtataaaaaaatggatgaatccgagaactatatgaacccactggaggaggaaggcagtaaatggaaagaggcactgtatcaaggatagaaaggtggtgaatactagatggaaagaggcactgtatcaaggatagaagttgaaattgtgaaagaaatggctcgtcaaatttctaattacgacaaagtgcttgaaatagtgaatcaaaaagataactttcgcagcattggcgaagttccgcttataccttggaaatcaactgctttgagtcatggtataccaggcatttgtatgctgtatggcgaattgcacgctcattttcctgaggaaggatgggacgacataggacatcaatatttatcgattttagtaaatgaaataaaagaaaaagggctgcatactccttcaatgttttctggagcagctggcattggactggcagcaatctgtttatcgcaacgtttcacctattacaatggtttgatttctgatattaatgaatatttggctgaaaccgtacctcaattgttaactgaatttgatcagcggcaagtgtgtatgagtgactacgatgtcatagaaggagttagcggaattgccaactatctgctgttatttcaagaagataaagctatgggagatttgttaattgatattttgaaatatctcgttaggttaactgaagacattatcgtggatggagaaaaagttcctggatggcatattccatctcaacatcagtttactgatattgaaaaaaaagcttatccttacggcaattttaatatgggattagcacatgggatacctggtcctatttgtgttctctcttcagcactcatacagggaattaaagtaaagggacaagaagctgcgattgaaaaaatggcgaatttcctactggaattttctgaaaaagagcaagacagcttgttttggaaaggaatcataagctttgaggagtatcaatacggatctccgccaaacgcagtaaattttagcagagatgcttggtgttatggaagacctggtgtatgtctagcgcttgttaaagccggaaaagcacttcaaaatactgaacttataaatatcggagtacagaatttaagatacacaatatctgatatacggggaattttttcacctaccatatgtcatggttacagcggtatcggtcagattctgcttgctgtaaatctactcaccggacaggagtattttaaggaagagcttcaagagattaaacaaaagatcatgagctactatgacaaggattatatctttggattccataactatgaatcaatggaaggggaggaagcagtacctttgcagtacgttggtttgttggatggagctgtaggtgtaggcttaggggtattaaacatggaattaggctcaaaaacagattggacaaaagcattattaatttaaaaaggtggtgaatactagatgtcaaagttcgatgatttcgatttggatgttgtgaaagtctctaaacaagactcaaaaatcactccgcaatggaaaagtgaatcactttgtacaccaggatgtgtaactggtgcattgcaaacttgcttccttcaaacactaacttgtaactgcaaaatctctaaataatcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K302027_sequence 1 atggaagtaaaggaacaactgaaactaaaagagctgctgtttatcatgaaacaaatgcctaagacgttcaagttgattttcaccttagaaagatcactgtttttaaagttgattcgattcagtattattaccggtattctgccaattgtttcactatatatttcacaagaactgattaattcccttgtgactatccggaaagaggtttcaattgttatcaccattttcttgacatatctaggggtatcttttttttcggagctcatttcgcagatttccgaattttataatggtaaatttcaattaaatattggttataaactcaactataaagtcatgaaaaagagcagtaatttagctctcaaggattttgaaaatccagaaatatatgacaagttagagagggtaacgaaagagatcagttataagccctatcagattattcaagccatcattacaatgaccacatcatttgtgacgctactctcatcaattgcgtttttaatgtcgtggaaccctaaagtctcacttctgttgttagttattccggtaatttctctattctatttcttgaaaattggacaggaagaatttttcatacactggaaacgcgcgggaaaggaaaggaaatcttggtatatcagctatatactcacacatgatttttcttttaaagaattaaaattgtataaccttaaggattatttattaaacaagtactgggatatcaaaaaatcatttatagaacaagatacaaagatattaagaaagaaaactttattgaacctaatatatgagattgcagtgcaattagtcggagctgtcattatatttattgccatcatgtctgcttttgcgggaaaaataatggtcggtaatgtaatgagttatatcagatcggtttcgctggtgcaaaatcactcacaatctatcatgacaagcatttattcgatctataacagcaatctctatatgaatcaattgtatgaatttctggaattaaaagaagagaaaagtcaaggtcacaagaagccgattgttgagcctattcattctgttgtttttcaaaatgtcagctttatctatcccaatcaaggagaacagacactgaaacatattaacgtttcattgcataaaggagaacgtgtagccattgttggaccaaacggatcagggaaaaaaacattcattaagctccttaccggcttatatgaagttcacgagggcgacattttgattaatggaataaatattaaagaactggatatggacagttatatgaatcaaattgcagcgctatttcaagactttatgaaatacgaaatgacattaaaagaaaatataggattcggccaaattgataaactacatcaaacaaacaaaatgcatgaagttctcgatattgtgagagccgatttcttaaaaagccactcttcctatcaattcgatacacagctcggactatggtttgatgaaggaagacagctatccggtggacaatggcaaaagattgctttggcaagggcttattttagggaagcttctttgtatattttggacgagccaagttctgcgctggatccaattgcagaaaaagaaaccttcgatacttttttcagtttgtcaaaagataaaataggaatatttatttctcatcgattggttgctgctaaacttgcagatcggatcattgttatggataaaggagagattgtaggaataggcacacatgaagagctattaaaaacatgtccgctgtataaaaaaatggatgaatccgagaactatatgaacccactggaggaggaaggcagtaaatggaaagaggcactgtatcaaggatag BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_K302028_sequence 1 atgaaatccttatatacacctacagattattatatgattcgggttcctttagtacatcaagacttaaaaaatgagaactctcaggatatcgatcagttattacatgacctttgcaacgattcattatttcgggaacaaatactggtatctagcaggacactatatgaaacaatacatactttcctgcaagcgccggataaattaaaagggaaaaaaaagcgcaactttcaacaggctattttgaagtatgcaacaagaagagcaacaagaaccacaccttttggccttttttcttcagtcggtatagggtcgttttctgataaaaatcacttatcttttaatcaacattcattttacaaaaaggctcgtgttgatttggagtggctctaccaattaattagaaaattggaaaacgaatacaccgaccgactttcttttacattaaattccgcttgttatattaagggtgaccgggcttacttgttgtacagcacagatggaaaatctgaagaagttagtgttcgtgcgacatctgttttctatttgataaatgaactgtgtggtgaatctgctgcatatcaagatataatccgttgtttgatagataactatccaaatactccaataaataaaattaatcagtacgtagcagaccttattgacaaagagttccttatatcaaacttacggccgccgatgactgtttcagatcaatttcagtatttaattgatcaagcggaaagccgccatattccaaatgaactcattcaggcttgtaaagacattcaatatcagatagatgcatataatcggatcactatcggagagggagagcatcagtatttaaatctgattgaaacaatgaataaactcataaaggcatcatctcctctgcaagtagacgctgggctggcagactcctccattcaattagataatgaaacatctctcgccataagtgaattggcaagcatgtttacttatatggcttctccctctgccaatacattagaccacttggaaaaataccacaatgtatttttggaacgctatggatatgaaagagaagttcctctcttagaaatgctatgttccagcactggcattggtgctcctgctacgtacacgaatcctgctaatgagttctttgaagaaacatcatttggggagcaattttcaccggaaatgaaacaatttttcatgagaaagtattttgaatcagttagaaagaaagctcctattcaattagacgacgaaacattccatagaatttgcaactctgaaattgctgatgaggaaattccattatcatttgaactcaatttctttgttaaattacgaaatgggagagttaagctttatttaggccccaacgtcggatctacccgcgcagggaaaacatttgggagattttctcatatgtctgattcaatcagtgaaatcataaagaccttacataacaaggagaaagagttaacagagtgtaatacgaaagtttgtgaactgagtattgtgcctaatcaaactaggtctggaaatgtaacaagaaatgtaagctaccgagaaaaagagatgtctctttttacgaacagtgctctgcatctcaatgattccgtcaaagctgaagatattctaattggaatcaataaagaccataacttttatgctagacataaaacaactggcgaaattctgtcttttgagtcaaatcatatgtttaatcctttattaatgaccaacgctgtacggtttttattagagatttcaagagatggtaaaagaaaatggaatgatttcccgtggtttagtatctatagtgatttcaagtatattcctgaaatcaaatataaagagataaccttatcttgtgaacaatggctgatatacaaaaatgatttaagcatgcactcgaatgcatcactagaagagataaaatctcctttttttgaatttcatcgtacttatgaactgccgcaaacattttatatcgttaacgcagacaatcgattattgattgatatagagaatgattgtactttggatgtttttttctgggaattgaaaaaaacgaaccataaccagccattacaacttgtggctgttgagcatgatgcagatgcgttaatggatagaaaccaaaatgactattcgggagaaatcgtcgttccgctgcttagaaaacaaccagaaaaaccattgtatttaccggttcttaacgcaatagagggaagtggttccgacagaataaaaatgccatttgaagactggctgtttattaagctttattgtaaacaaacaagagaagaagagctaattgcttttgaaatagcggatttttataaccagatttctgatcaatatccagtcagacatttctttatgaggtatcgggatccaaagcctcatataagacttagatttaatggaaaagccgaagtgctgtacagcttgtttccccaattattgaattggctgaaaagcttaagagaaaaaggactggtttcagagtctgttatcactcaatacgagcgggagatagaacgatatggcgggctaagccttatggaggctgcggaacagcttttctgtgaagacagcaaagttgttgaaatgatgattagaatgcaccggatgaaagatattacgataagcaaggaaattgcaggcatggtttcggttatacagtttttagaacagttcgagctaacgtttgaagaacagttaacttttttagagagaaattccttacagaatgagtatcgtactgaatttaaaaaggatagagaaatgtatattgaaatatgcaattctgacagagattgggataatctcaagaaaacaagtgatggcggtatgttatatgaaactttgaaaacaagaaaaatggctgcggctcattatgcatttttaatcaaaaaggcatttgataacaaagatgaagtttattcacgtataggaagtatcatccatctgcattgcaatcgtttattcggaaccgacagagaactggaaaataaaattctcaccctatgcagacattctttatatgcgcaacgatatcaaaagatgaatggtagtttagcatggaagtaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z