##gff-version 3
##sequence-region BBa_K302021 1 6683
BBa_K302021	.	ribosome_entry_site	5056	5067	.	+	0	ID=BBa_K143021;Name=BBa_K143021
BBa_K302021	.	ribosome_entry_site	5056	5067	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K302021	.	CDS	6418	6588	.	+	0	ID=BBa_K302023;Name=BBa_K302023
BBa_K302021	.	ribosome_entry_site	3193	3204	.	+	0	ID=BBa_K143021;Name=BBa_K143021
BBa_K302021	.	ribosome_entry_site	3193	3204	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K302021	.	sequence_feature	94	99	.	+	0	ID=BBa_G0000;Name=BBa_G0000
BBa_K302021	.	ribosome_entry_site	82	93	.	+	0	ID=BBa_K143021;Name=BBa_K143021
BBa_K302021	.	ribosome_entry_site	82	93	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K302021	.	sequence_feature	6412	6417	.	+	0	ID=BBa_G0000;Name=BBa_G0000
BBa_K302021	.	promoter	1	81	.	+	0	ID=BBa_K302020;Name=BBa_K302020
BBa_K302021	.	sequence_feature	5068	5073	.	+	0	ID=BBa_G0000;Name=BBa_G0000
BBa_K302021	.	CDS	3211	5055	.	+	0	ID=BBa_K302027;Name=BBa_K302027
BBa_K302021	.	point_mutation	3804	3804	.	+	0	ID=annotation2081984;Name=restriction site removed;Parent=BBa_K302027
BBa_K302021	.	CDS	5074	6399	.	+	0	ID=BBa_K302026;Name=BBa_K302026
BBa_K302021	.	ribosome_entry_site	6400	6411	.	+	0	ID=BBa_K143021;Name=BBa_K143021
BBa_K302021	.	ribosome_entry_site	6400	6411	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K302021	.	CDS	100	3192	.	+	0	ID=BBa_K302028;Name=BBa_K302028
BBa_K302021	.	point_mutation	177	177	.	+	0	ID=annotation2081979;Name=restriction site removed;Parent=BBa_K302028
BBa_K302021	.	point_mutation	2673	2673	.	+	0	ID=annotation2081982;Name=restriction site removed;Parent=BBa_K302028
BBa_K302021	.	point_mutation	2019	2019	.	+	0	ID=annotation2081981;Name=restriction site removed;Parent=BBa_K302028
BBa_K302021	.	point_mutation	1242	1242	.	+	0	ID=annotation2081980;Name=restriction site removed;Parent=BBa_K302028
BBa_K302021	.	point_mutation	2991	2991	.	+	0	ID=annotation2081983;Name=restriction site removed;Parent=BBa_K302028
BBa_K302021	.	sequence_feature	3205	3210	.	+	0	ID=BBa_G0000;Name=BBa_G0000
BBa_K302021	.	terminator	6589	6683	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K302021	.	terminator	6589	6629	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K302021	.	engineered_region	6589	6629	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K302021	.	stop_codon	6622	6622	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K302021	.	stem_loop	6596	6615	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K302021	.	polyA_site	6616	6629	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K302021	.	terminator	6638	6683	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K302021	.	stem_loop	6650	6672	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K302021	.	engineered_region	6638	6683	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
>BBa_K302021
cactggggcattcacaagattgttagttttttctcgatgggtacaaatcaattcacgttacaataaacacacaacgtga
ctaaaggtggtgaatactagatgaaatccttatatacacctacagattattatatgattcgggttcctttagtacatca
agacttaaaaaatgagaactctcaggatatcgatcagttattacatgacctttgcaacgattcattatttcgggaacaa
atactggtatctagcaggacactatatgaaacaatacatactttcctgcaagcgccggataaattaaaagggaaaaaaa
agcgcaactttcaacaggctattttgaagtatgcaacaagaagagcaacaagaaccacaccttttggccttttttcttc
agtcggtatagggtcgttttctgataaaaatcacttatcttttaatcaacattcattttacaaaaaggctcgtgttgat
ttggagtggctctaccaattaattagaaaattggaaaacgaatacaccgaccgactttcttttacattaaattccgctt
gttatattaagggtgaccgggcttacttgttgtacagcacagatggaaaatctgaagaagttagtgttcgtgcgacatc
tgttttctatttgataaatgaactgtgtggtgaatctgctgcatatcaagatataatccgttgtttgatagataactat
ccaaatactccaataaataaaattaatcagtacgtagcagaccttattgacaaagagttccttatatcaaacttacggc
cgccgatgactgtttcagatcaatttcagtatttaattgatcaagcggaaagccgccatattccaaatgaactcattca
ggcttgtaaagacattcaatatcagatagatgcatataatcggatcactatcggagagggagagcatcagtatttaaat
ctgattgaaacaatgaataaactcataaaggcatcatctcctctgcaagtagacgctgggctggcagactcctccattc
aattagataatgaaacatctctcgccataagtgaattggcaagcatgtttacttatatggcttctccctctgccaatac
attagaccacttggaaaaataccacaatgtatttttggaacgctatggatatgaaagagaagttcctctcttagaaatg
ctatgttccagcactggcattggtgctcctgctacgtacacgaatcctgctaatgagttctttgaagaaacatcatttg
gggagcaattttcaccggaaatgaaacaatttttcatgagaaagtattttgaatcagttagaaagaaagctcctattca
attagacgacgaaacattccatagaatttgcaactctgaaattgctgatgaggaaattccattatcatttgaactcaat
ttctttgttaaattacgaaatgggagagttaagctttatttaggccccaacgtcggatctacccgcgcagggaaaacat
ttgggagattttctcatatgtctgattcaatcagtgaaatcataaagaccttacataacaaggagaaagagttaacaga
gtgtaatacgaaagtttgtgaactgagtattgtgcctaatcaaactaggtctggaaatgtaacaagaaatgtaagctac
cgagaaaaagagatgtctctttttacgaacagtgctctgcatctcaatgattccgtcaaagctgaagatattctaattg
gaatcaataaagaccataacttttatgctagacataaaacaactggcgaaattctgtcttttgagtcaaatcatatgtt
taatcctttattaatgaccaacgctgtacggtttttattagagatttcaagagatggtaaaagaaaatggaatgatttc
ccgtggtttagtatctatagtgatttcaagtatattcctgaaatcaaatataaagagataaccttatcttgtgaacaat
ggctgatatacaaaaatgatttaagcatgcactcgaatgcatcactagaagagataaaatctcctttttttgaatttca
tcgtacttatgaactgccgcaaacattttatatcgttaacgcagacaatcgattattgattgatatagagaatgattgt
actttggatgtttttttctgggaattgaaaaaaacgaaccataaccagccattacaacttgtggctgttgagcatgatg
cagatgcgttaatggatagaaaccaaaatgactattcgggagaaatcgtcgttccgctgcttagaaaacaaccagaaaa
accattgtatttaccggttcttaacgcaatagagggaagtggttccgacagaataaaaatgccatttgaagactggctg
tttattaagctttattgtaaacaaacaagagaagaagagctaattgcttttgaaatagcggatttttataaccagattt
ctgatcaatatccagtcagacatttctttatgaggtatcgggatccaaagcctcatataagacttagatttaatggaaa
agccgaagtgctgtacagcttgtttccccaattattgaattggctgaaaagcttaagagaaaaaggactggtttcagag
tctgttatcactcaatacgagcgggagatagaacgatatggcgggctaagccttatggaggctgcggaacagcttttct
gtgaagacagcaaagttgttgaaatgatgattagaatgcaccggatgaaagatattacgataagcaaggaaattgcagg
catggtttcggttatacagtttttagaacagttcgagctaacgtttgaagaacagttaacttttttagagagaaattcc
ttacagaatgagtatcgtactgaatttaaaaaggatagagaaatgtatattgaaatatgcaattctgacagagattggg
ataatctcaagaaaacaagtgatggcggtatgttatatgaaactttgaaaacaagaaaaatggctgcggctcattatgc
atttttaatcaaaaaggcatttgataacaaagatgaagtttattcacgtataggaagtatcatccatctgcattgcaat
cgtttattcggaaccgacagagaactggaaaataaaattctcaccctatgcagacattctttatatgcgcaacgatatc
aaaagatgaatggtagtttagcatggaagtaaaaaggtggtgaatactagatggaagtaaaggaacaactgaaactaaa
agagctgctgtttatcatgaaacaaatgcctaagacgttcaagttgattttcaccttagaaagatcactgtttttaaag
ttgattcgattcagtattattaccggtattctgccaattgtttcactatatatttcacaagaactgattaattcccttg
tgactatccggaaagaggtttcaattgttatcaccattttcttgacatatctaggggtatcttttttttcggagctcat
ttcgcagatttccgaattttataatggtaaatttcaattaaatattggttataaactcaactataaagtcatgaaaaag
agcagtaatttagctctcaaggattttgaaaatccagaaatatatgacaagttagagagggtaacgaaagagatcagtt
ataagccctatcagattattcaagccatcattacaatgaccacatcatttgtgacgctactctcatcaattgcgttttt
aatgtcgtggaaccctaaagtctcacttctgttgttagttattccggtaatttctctattctatttcttgaaaattgga
caggaagaatttttcatacactggaaacgcgcgggaaaggaaaggaaatcttggtatatcagctatatactcacacatg
atttttcttttaaagaattaaaattgtataaccttaaggattatttattaaacaagtactgggatatcaaaaaatcatt
tatagaacaagatacaaagatattaagaaagaaaactttattgaacctaatatatgagattgcagtgcaattagtcgga
gctgtcattatatttattgccatcatgtctgcttttgcgggaaaaataatggtcggtaatgtaatgagttatatcagat
cggtttcgctggtgcaaaatcactcacaatctatcatgacaagcatttattcgatctataacagcaatctctatatgaa
tcaattgtatgaatttctggaattaaaagaagagaaaagtcaaggtcacaagaagccgattgttgagcctattcattct
gttgtttttcaaaatgtcagctttatctatcccaatcaaggagaacagacactgaaacatattaacgtttcattgcata
aaggagaacgtgtagccattgttggaccaaacggatcagggaaaaaaacattcattaagctccttaccggcttatatga
agttcacgagggcgacattttgattaatggaataaatattaaagaactggatatggacagttatatgaatcaaattgca
gcgctatttcaagactttatgaaatacgaaatgacattaaaagaaaatataggattcggccaaattgataaactacatc
aaacaaacaaaatgcatgaagttctcgatattgtgagagccgatttcttaaaaagccactcttcctatcaattcgatac
acagctcggactatggtttgatgaaggaagacagctatccggtggacaatggcaaaagattgctttggcaagggcttat
tttagggaagcttctttgtatattttggacgagccaagttctgcgctggatccaattgcagaaaaagaaaccttcgata
cttttttcagtttgtcaaaagataaaataggaatatttatttctcatcgattggttgctgctaaacttgcagatcggat
cattgttatggataaaggagagattgtaggaataggcacacatgaagagctattaaaaacatgtccgctgtataaaaaa
atggatgaatccgagaactatatgaacccactggaggaggaaggcagtaaatggaaagaggcactgtatcaaggataga
aaggtggtgaatactagatggaaagaggcactgtatcaaggatagaagttgaaattgtgaaagaaatggctcgtcaaat
ttctaattacgacaaagtgcttgaaatagtgaatcaaaaagataactttcgcagcattggcgaagttccgcttatacct
tggaaatcaactgctttgagtcatggtataccaggcatttgtatgctgtatggcgaattgcacgctcattttcctgagg
aaggatgggacgacataggacatcaatatttatcgattttagtaaatgaaataaaagaaaaagggctgcatactccttc
aatgttttctggagcagctggcattggactggcagcaatctgtttatcgcaacgtttcacctattacaatggtttgatt
tctgatattaatgaatatttggctgaaaccgtacctcaattgttaactgaatttgatcagcggcaagtgtgtatgagtg
actacgatgtcatagaaggagttagcggaattgccaactatctgctgttatttcaagaagataaagctatgggagattt
gttaattgatattttgaaatatctcgttaggttaactgaagacattatcgtggatggagaaaaagttcctggatggcat
attccatctcaacatcagtttactgatattgaaaaaaaagcttatccttacggcaattttaatatgggattagcacatg
ggatacctggtcctatttgtgttctctcttcagcactcatacagggaattaaagtaaagggacaagaagctgcgattga
aaaaatggcgaatttcctactggaattttctgaaaaagagcaagacagcttgttttggaaaggaatcataagctttgag
gagtatcaatacggatctccgccaaacgcagtaaattttagcagagatgcttggtgttatggaagacctggtgtatgtc
tagcgcttgttaaagccggaaaagcacttcaaaatactgaacttataaatatcggagtacagaatttaagatacacaat
atctgatatacggggaattttttcacctaccatatgtcatggttacagcggtatcggtcagattctgcttgctgtaaat
ctactcaccggacaggagtattttaaggaagagcttcaagagattaaacaaaagatcatgagctactatgacaaggatt
atatctttggattccataactatgaatcaatggaaggggaggaagcagtacctttgcagtacgttggtttgttggatgg
agctgtaggtgtaggcttaggggtattaaacatggaattaggctcaaaaacagattggacaaaagcattattaatttaa
aaaggtggtgaatactagatgtcaaagttcgatgatttcgatttggatgttgtgaaagtctctaaacaagactcaaaaa
tcactccgcaatggaaaagtgaatcactttgtacaccaggatgtgtaactggtgcattgcaaacttgcttccttcaaac
actaacttgtaactgcaaaatctctaaataatcacactggctcaccttcgggtgggcctttctgcgtttatatactaga
gagagaatataaaaagccagattattaatccggcttttttattattt