BBa_K302021
type: ComponentDefinition
persistent identity: BBa K302021
display id: BBa_K302021
version: 1
was generated by: iGEM to SBOL conversion
description: Oxygen-limitation induced subtilin production
created: 2010-09-24T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: ..
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 0
owner id: 6009
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: .
star: false
creator: Alan Koh, Philip Hall, Steven Woodhouse, Rachel May Boyd
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K143021
component: BBa_G0000
component: BBa_K302027
component: BBa_K302020
component: BBa_K302028
component: BBa_B0014
component: BBa_K302023
component: BBa_K302026
sequence annotation: BBa_K302020
sequence annotation: BBa_K143021
sequence annotation: BBa_G0000
sequence annotation: BBa_K302028
sequence annotation: BBa_K302027
sequence annotation: BBa_K302026
sequence annotation: BBa_K302023
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K302021_sequence/1

BBa_K143021
type: Component
persistent identity: component2235390
display id: component2235390
version: 1
top level: BBa_K302021
definition: RBS-spoVG
access: public

BBa_G0000
type: Component
persistent identity: component2235382
display id: component2235382
version: 1
top level: BBa_K302021
definition: scar
access: public

BBa_K143021
type: Component
persistent identity: component2235381
display id: component2235381
version: 1
top level: BBa_K302021
definition: RBS-spoVG
access: public

BBa_K143021
type: Component
persistent identity: component2235386
display id: component2235386
version: 1
top level: BBa_K302021
definition: RBS-spoVG
access: public

BBa_K302027
type: Component
persistent identity: component2235384
display id: component2235384
version: 1
top level: BBa_K302021
definition: BBa_K302027
access: public

BBa_K143021
type: Component
persistent identity: component2235372
display id: component2235372
version: 1
top level: BBa_K302021
definition: RBS-spoVG
access: public

BBa_K302020
type: Component
persistent identity: component2235370
display id: component2235370
version: 1
top level: BBa_K302021
definition: BBa_K302020
access: public

BBa_K302028
type: Component
persistent identity: component2235379
display id: component2235379
version: 1
top level: BBa_K302021
definition: BBa_K302028
access: public

BBa_G0000
type: Component
persistent identity: component2235373
display id: component2235373
version: 1
top level: BBa_K302021
definition: scar
access: public

BBa_B0014
type: Component
persistent identity: component2235399
display id: component2235399
version: 1
top level: BBa_K302021
definition: BBa_B0014
access: public

BBa_K302023
type: Component
persistent identity: component2235392
display id: component2235392
version: 1
top level: BBa_K302021
definition: BBa_K302023
access: public

BBa_K302026
type: Component
persistent identity: component2235388
display id: component2235388
version: 1
top level: BBa_K302021
definition: BBa_K302026
access: public

BBa_G0000
type: Component
persistent identity: component2235387
display id: component2235387
version: 1
top level: BBa_K302021
definition: scar
access: public

BBa_G0000
type: Component
persistent identity: component2235391
display id: component2235391
version: 1
top level: BBa_K302021
definition: scar
access: public

BBa_K302020
type: SequenceAnnotation
persistent identity: annotation2235370
display id: annotation2235370
version: 1
top level: BBa_K302021
location: https://synbiohub.org/public/igem/BBa_K302021/annotation2235370/range2235370/1
component: BBa_K302020

https://synbiohub.org/public/igem/BBa_K302021/annotation2235370/range2235370/1
type: Range
persistent identity: range2235370
display id: range2235370
version: 1
top level: BBa_K302021
direction: direction/reverse
start: 1
end: 81
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2235372
display id: annotation2235372
version: 1
top level: BBa_K302021
location: https://synbiohub.org/public/igem/BBa_K302021/annotation2235372/range2235372/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K302021/annotation2235372/range2235372/1
type: Range
persistent identity: range2235372
display id: range2235372
version: 1
top level: BBa_K302021
direction: direction/reverse
start: 82
end: 93
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2235373
display id: annotation2235373
version: 1
top level: BBa_K302021
location: https://synbiohub.org/public/igem/BBa_K302021/annotation2235373/range2235373/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K302021/annotation2235373/range2235373/1
type: Range
persistent identity: range2235373
display id: range2235373
version: 1
top level: BBa_K302021
direction: direction/reverse
start: 94
end: 99
orientation: inline

BBa_K302028
type: SequenceAnnotation
persistent identity: annotation2235379
display id: annotation2235379
version: 1
top level: BBa_K302021
location: https://synbiohub.org/public/igem/BBa_K302021/annotation2235379/range2235379/1
component: BBa_K302028

https://synbiohub.org/public/igem/BBa_K302021/annotation2235379/range2235379/1
type: Range
persistent identity: range2235379
display id: range2235379
version: 1
top level: BBa_K302021
direction: direction/reverse
start: 100
end: 3192
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2235381
display id: annotation2235381
version: 1
top level: BBa_K302021
location: https://synbiohub.org/public/igem/BBa_K302021/annotation2235381/range2235381/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K302021/annotation2235381/range2235381/1
type: Range
persistent identity: range2235381
display id: range2235381
version: 1
top level: BBa_K302021
direction: direction/reverse
start: 3193
end: 3204
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2235382
display id: annotation2235382
version: 1
top level: BBa_K302021
location: https://synbiohub.org/public/igem/BBa_K302021/annotation2235382/range2235382/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K302021/annotation2235382/range2235382/1
type: Range
persistent identity: range2235382
display id: range2235382
version: 1
top level: BBa_K302021
direction: direction/reverse
start: 3205
end: 3210
orientation: inline

BBa_K302027
type: SequenceAnnotation
persistent identity: annotation2235384
display id: annotation2235384
version: 1
top level: BBa_K302021
location: https://synbiohub.org/public/igem/BBa_K302021/annotation2235384/range2235384/1
component: BBa_K302027

https://synbiohub.org/public/igem/BBa_K302021/annotation2235384/range2235384/1
type: Range
persistent identity: range2235384
display id: range2235384
version: 1
top level: BBa_K302021
direction: direction/reverse
start: 3211
end: 5055
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2235386
display id: annotation2235386
version: 1
top level: BBa_K302021
location: https://synbiohub.org/public/igem/BBa_K302021/annotation2235386/range2235386/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K302021/annotation2235386/range2235386/1
type: Range
persistent identity: range2235386
display id: range2235386
version: 1
top level: BBa_K302021
direction: direction/reverse
start: 5056
end: 5067
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2235387
display id: annotation2235387
version: 1
top level: BBa_K302021
location: https://synbiohub.org/public/igem/BBa_K302021/annotation2235387/range2235387/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K302021/annotation2235387/range2235387/1
type: Range
persistent identity: range2235387
display id: range2235387
version: 1
top level: BBa_K302021
direction: direction/reverse
start: 5068
end: 5073
orientation: inline

BBa_K302026
type: SequenceAnnotation
persistent identity: annotation2235388
display id: annotation2235388
version: 1
top level: BBa_K302021
location: https://synbiohub.org/public/igem/BBa_K302021/annotation2235388/range2235388/1
component: BBa_K302026

https://synbiohub.org/public/igem/BBa_K302021/annotation2235388/range2235388/1
type: Range
persistent identity: range2235388
display id: range2235388
version: 1
top level: BBa_K302021
direction: direction/reverse
start: 5074
end: 6399
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation2235390
display id: annotation2235390
version: 1
top level: BBa_K302021
location: https://synbiohub.org/public/igem/BBa_K302021/annotation2235390/range2235390/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K302021/annotation2235390/range2235390/1
type: Range
persistent identity: range2235390
display id: range2235390
version: 1
top level: BBa_K302021
direction: direction/reverse
start: 6400
end: 6411
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2235391
display id: annotation2235391
version: 1
top level: BBa_K302021
location: https://synbiohub.org/public/igem/BBa_K302021/annotation2235391/range2235391/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K302021/annotation2235391/range2235391/1
type: Range
persistent identity: range2235391
display id: range2235391
version: 1
top level: BBa_K302021
direction: direction/reverse
start: 6412
end: 6417
orientation: inline

BBa_K302023
type: SequenceAnnotation
persistent identity: annotation2235392
display id: annotation2235392
version: 1
top level: BBa_K302021
location: https://synbiohub.org/public/igem/BBa_K302021/annotation2235392/range2235392/1
component: BBa_K302023

https://synbiohub.org/public/igem/BBa_K302021/annotation2235392/range2235392/1
type: Range
persistent identity: range2235392
display id: range2235392
version: 1
top level: BBa_K302021
direction: direction/reverse
start: 6418
end: 6588
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2235399
display id: annotation2235399
version: 1
top level: BBa_K302021
location: https://synbiohub.org/public/igem/BBa_K302021/annotation2235399/range2235399/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K302021/annotation2235399/range2235399/1
type: Range
persistent identity: range2235399
display id: range2235399
version: 1
top level: BBa_K302021
direction: direction/reverse
start: 6589
end: 6683
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa G0000
display id: BBa_G0000
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/XbaI scar for RBS-CDS junctions
created: 2007-07-22T11:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: SpeI/XbaI scar
owned by: james
owned by: myers
mutable description: This is the sequence of the SpeI/XbaI scar for RBS-CDS junctions in BioBricks standard assembly.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a shorter scar to ensure proper spacing between the RBS and CDS.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0000_sequence/1

RBS-spoVG
type: ComponentDefinition
persistent identity: BBa K143021
display id: BBa_K143021
version: 1
was generated by: iGEM to SBOL conversion
description: SpoVG ribosome binding site (RBS) for B. subtilis
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143021_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975997
display id: annotation1975997
version: 1
top level: RBS-spoVG
location: https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
type: Range
persistent identity: range1975997
display id: range1975997
version: 1
top level: RBS-spoVG
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_K302020
type: ComponentDefinition
persistent identity: BBa K302020
display id: BBa_K302020
version: 1
was generated by: iGEM to SBOL conversion
description: Oxygen-limitation activated promoter
created: 2010-09-24T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 0
owner id: 6009
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: .
star: false
creator: Philip Hall, Alan Koh, Steven Woodhouse
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K302020_sequence/1

BBa_K302027
type: ComponentDefinition
persistent identity: BBa K302027
display id: BBa_K302027
version: 1
was generated by: iGEM to SBOL conversion
description: spaT
created: 2010-10-03T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
group u list: _442_
m user id: 0
owner id: 6009
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: .
star: false
creator: Steven Woodhouse
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: restriction site removed
sequence: https://synbiohub.org/public/igem/BBa_K302027_sequence/1

restriction site removed
type: SequenceAnnotation
persistent identity: annotation2081984
display id: annotation2081984
version: 1
top level: BBa_K302027
location: https://synbiohub.org/public/igem/BBa_K302027/annotation2081984/range2081984/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K302027/annotation2081984/range2081984/1
type: Range
persistent identity: range2081984
display id: range2081984
version: 1
top level: BBa_K302027
direction: direction/forward
start: 594
end: 594
orientation: inline

BBa_K302028
type: ComponentDefinition
persistent identity: BBa K302028
display id: BBa_K302028
version: 1
was generated by: iGEM to SBOL conversion
description: spaB
created: 2010-10-03T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
experience: experience/None
group u list: _442_
m user id: 0
owner id: 6009
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: .
star: false
creator: Steven Woodhouse
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: restriction site removed
sequence: https://synbiohub.org/public/igem/BBa_K302028_sequence/1

restriction site removed
type: SequenceAnnotation
persistent identity: annotation2081979
display id: annotation2081979
version: 1
top level: BBa_K302028
location: https://synbiohub.org/public/igem/BBa_K302028/annotation2081979/range2081979/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K302028/annotation2081979/range2081979/1
type: Range
persistent identity: range2081979
display id: range2081979
version: 1
top level: BBa_K302028
direction: direction/forward
start: 78
end: 78
orientation: inline

restriction site removed
type: SequenceAnnotation
persistent identity: annotation2081980
display id: annotation2081980
version: 1
top level: BBa_K302028
location: https://synbiohub.org/public/igem/BBa_K302028/annotation2081980/range2081980/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K302028/annotation2081980/range2081980/1
type: Range
persistent identity: range2081980
display id: range2081980
version: 1
top level: BBa_K302028
direction: direction/forward
start: 1143
end: 1143
orientation: inline

restriction site removed
type: SequenceAnnotation
persistent identity: annotation2081981
display id: annotation2081981
version: 1
top level: BBa_K302028
location: https://synbiohub.org/public/igem/BBa_K302028/annotation2081981/range2081981/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K302028/annotation2081981/range2081981/1
type: Range
persistent identity: range2081981
display id: range2081981
version: 1
top level: BBa_K302028
direction: direction/forward
start: 1920
end: 1920
orientation: inline

restriction site removed
type: SequenceAnnotation
persistent identity: annotation2081982
display id: annotation2081982
version: 1
top level: BBa_K302028
location: https://synbiohub.org/public/igem/BBa_K302028/annotation2081982/range2081982/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K302028/annotation2081982/range2081982/1
type: Range
persistent identity: range2081982
display id: range2081982
version: 1
top level: BBa_K302028
direction: direction/forward
start: 2574
end: 2574
orientation: inline

restriction site removed
type: SequenceAnnotation
persistent identity: annotation2081983
display id: annotation2081983
version: 1
top level: BBa_K302028
location: https://synbiohub.org/public/igem/BBa_K302028/annotation2081983/range2081983/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K302028/annotation2081983/range2081983/1
type: Range
persistent identity: range2081983
display id: range2081983
version: 1
top level: BBa_K302028
direction: direction/forward
start: 2892
end: 2892
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_K302023
type: ComponentDefinition
persistent identity: BBa K302023
display id: BBa_K302023
version: 1
was generated by: iGEM to SBOL conversion
description: spaS
created: 2010-10-02T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
group u list: _442_
m user id: 0
owner id: 6009
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: .
star: false
creator: Steven Woodhouse
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K302023_sequence/1

BBa_K302026
type: ComponentDefinition
persistent identity: BBa K302026
display id: BBa_K302026
version: 1
was generated by: iGEM to SBOL conversion
description: spaC
created: 2010-10-03T11:00:00Z
modified: 2015-05-08T01:11:52Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
group u list: _442_
m user id: 0
owner id: 6009
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: .
star: false
creator: Steven Woodhouse
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K302026_sequence/1

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K302021_sequence/1
type: Sequence
persistent identity: BBa K302021 sequence
display id: BBa_K302021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cactggggcattcacaagattgttagttttttctcgatgggtacaaatcaattcacgttacaataaacacacaacgtgactaaaggtggtgaatactagatgaaatccttatatacacctacagattattatatgattcgggttcctttagtacatcaagacttaaaaaatgagaactctcaggatatcgatcagttattacatgacctttgcaacgattcattatttcgggaacaaatactggtatctagcaggacactatatgaaacaatacatactttcctgcaagcgccggataaattaaaagggaaaaaaaagcgcaactttcaacaggctattttgaagtatgcaacaagaagagcaacaagaaccacaccttttggccttttttcttcagtcggtatagggtcgttttctgataaaaatcacttatcttttaatcaacattcattttacaaaaaggctcgtgttgatttggagtggctctaccaattaattagaaaattggaaaacgaatacaccgaccgactttcttttacattaaattccgcttgttatattaagggtgaccgggcttacttgttgtacagcacagatggaaaatctgaagaagttagtgttcgtgcgacatctgttttctatttgataaatgaactgtgtggtgaatctgctgcatatcaagatataatccgttgtttgatagataactatccaaatactccaataaataaaattaatcagtacgtagcagaccttattgacaaagagttccttatatcaaacttacggccgccgatgactgtttcagatcaatttcagtatttaattgatcaagcggaaagccgccatattccaaatgaactcattcaggcttgtaaagacattcaatatcagatagatgcatataatcggatcactatcggagagggagagcatcagtatttaaatctgattgaaacaatgaataaactcataaaggcatcatctcctctgcaagtagacgctgggctggcagactcctccattcaattagataatgaaacatctctcgccataagtgaattggcaagcatgtttacttatatggcttctccctctgccaatacattagaccacttggaaaaataccacaatgtatttttggaacgctatggatatgaaagagaagttcctctcttagaaatgctatgttccagcactggcattggtgctcctgctacgtacacgaatcctgctaatgagttctttgaagaaacatcatttggggagcaattttcaccggaaatgaaacaatttttcatgagaaagtattttgaatcagttagaaagaaagctcctattcaattagacgacgaaacattccatagaatttgcaactctgaaattgctgatgaggaaattccattatcatttgaactcaatttctttgttaaattacgaaatgggagagttaagctttatttaggccccaacgtcggatctacccgcgcagggaaaacatttgggagattttctcatatgtctgattcaatcagtgaaatcataaagaccttacataacaaggagaaagagttaacagagtgtaatacgaaagtttgtgaactgagtattgtgcctaatcaaactaggtctggaaatgtaacaagaaatgtaagctaccgagaaaaagagatgtctctttttacgaacagtgctctgcatctcaatgattccgtcaaagctgaagatattctaattggaatcaataaagaccataacttttatgctagacataaaacaactggcgaaattctgtcttttgagtcaaatcatatgtttaatcctttattaatgaccaacgctgtacggtttttattagagatttcaagagatggtaaaagaaaatggaatgatttcccgtggtttagtatctatagtgatttcaagtatattcctgaaatcaaatataaagagataaccttatcttgtgaacaatggctgatatacaaaaatgatttaag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0000_sequence/1
type: Sequence
persistent identity: BBa G0000 sequence
display id: BBa_G0000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143021_sequence/1
type: Sequence
persistent identity: BBa K143021 sequence
display id: BBa_K143021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggtggtgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302020_sequence/1
type: Sequence
persistent identity: BBa K302020 sequence
display id: BBa_K302020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cactggggcattcacaagattgttagttttttctcgatgggtacaaatcaattcacgttacaataaacacacaacgtgact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302027_sequence/1
type: Sequence
persistent identity: BBa K302027 sequence
display id: BBa_K302027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaagtaaaggaacaactgaaactaaaagagctgctgtttatcatgaaacaaatgcctaagacgttcaagttgattttcaccttagaaagatcactgtttttaaagttgattcgattcagtattattaccggtattctgccaattgtttcactatatatttcacaagaactgattaattcccttgtgactatccggaaagaggtttcaattgttatcaccattttcttgacatatctaggggtatcttttttttcggagctcatttcgcagatttccgaattttataatggtaaatttcaattaaatattggttataaactcaactataaagtcatgaaaaagagcagtaatttagctctcaaggattttgaaaatccagaaatatatgacaagttagagagggtaacgaaagagatcagttataagccctatcagattattcaagccatcattacaatgaccacatcatttgtgacgctactctcatcaattgcgtttttaatgtcgtggaaccctaaagtctcacttctgttgttagttattccggtaatttctctattctatttcttgaaaattggacaggaagaatttttcatacactggaaacgcgcgggaaaggaaaggaaatcttggtatatcagctatatactcacacatgatttttcttttaaagaattaaaattgtataaccttaaggattatttattaaacaagtactgggatatcaaaaaatcatttatagaacaagatacaaagatattaagaaagaaaactttattgaacctaatatatgagattgcagtgcaattagtcggagctgtcattatatttattgccatcatgtctgcttttgcgggaaaaataatggtcggtaatgtaatgagttatatcagatcggtttcgctggtgcaaaatcactcacaatctatcatgacaagcatttattcgatctataacagcaatctctatatgaatcaattgtatgaatttctggaattaaaagaagagaaaagtcaaggtcacaagaagccgattgttgagcctattcattctgttgtttttcaaaatgtcagctttatctatcccaatcaaggagaacagacactgaaacatattaacgtttcattgcataaaggagaacgtgtagccattgttggaccaaacggatcagggaaaaaaacattcattaagctccttaccggcttatatgaagttcacgagggcgacattttgattaatggaataaatattaaagaactggatatggacagttatatgaatcaaattgcagcgctatttcaagactttatgaaatacgaaatgacattaaaagaaaatataggattcggccaaattgataaactacatcaaacaaacaaaatgcatgaagttctcgatattgtgagagccgatttcttaaaaagccactcttcctatcaattcgatacacagctcggactatggtttgatgaaggaagacagctatccggtggacaatggcaaaagattgctttggcaagggcttattttagggaagcttctttgtatattttggacgagccaagttctgcgctggatccaattgcagaaaaagaaaccttcgatacttttttcagtttgtcaaaagataaaataggaatatttatttctcatcgattggttgctgctaaacttgcagatcggatcattgttatggataaaggagagattgtaggaataggcacacatgaagagctattaaaaacatgtccgctgtataaaaaaatggatgaatccgagaactatatgaacccactggaggaggaaggcagtaaatggaaagaggcactgtatcaaggatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302023_sequence/1
type: Sequence
persistent identity: BBa K302023 sequence
display id: BBa_K302023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcaaagttcgatgatttcgatttggatgttgtgaaagtctctaaacaagactcaaaaatcactccgcaatggaaaagtgaatcactttgtacaccaggatgtgtaactggtgcattgcaaacttgcttccttcaaacactaacttgtaactgcaaaatctctaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302026_sequence/1
type: Sequence
persistent identity: BBa K302026 sequence
display id: BBa_K302026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaaagaggcactgtatcaaggatagaagttgaaattgtgaaagaaatggctcgtcaaatttctaattacgacaaagtgcttgaaatagtgaatcaaaaagataactttcgcagcattggcgaagttccgcttataccttggaaatcaactgctttgagtcatggtataccaggcatttgtatgctgtatggcgaattgcacgctcattttcctgaggaaggatgggacgacataggacatcaatatttatcgattttagtaaatgaaataaaagaaaaagggctgcatactccttcaatgttttctggagcagctggcattggactggcagcaatctgtttatcgcaacgtttcacctattacaatggtttgatttctgatattaatgaatatttggctgaaaccgtacctcaattgttaactgaatttgatcagcggcaagtgtgtatgagtgactacgatgtcatagaaggagttagcggaattgccaactatctgctgttatttcaagaagataaagctatgggagatttgttaattgatattttgaaatatctcgttaggttaactgaagacattatcgtggatggagaaaaagttcctggatggcatattccatctcaacatcagtttactgatattgaaaaaaaagcttatccttacggcaattttaatatgggattagcacatgggatacctggtcctatttgtgttctctcttcagcactcatacagggaattaaagtaaagggacaagaagctgcgattgaaaaaatggcgaatttcctactggaattttctgaaaaagagcaagacagcttgttttggaaaggaatcataagctttgaggagtatcaatacggatctccgccaaacgcagtaaattttagcagagatgcttggtgttatggaagacctggtgtatgtctagcgcttgttaaagccggaaaagcacttcaaaatactgaacttataaatatcggagtacagaatttaagatacacaatatctgatatacggggaattttttcacctaccatatgtcatggttacagcggtatcggtcagattctgcttgctgtaaatctactcaccggacaggagtattttaaggaagagcttcaagagattaaacaaaagatcatgagctactatgacaaggattatatctttggattccataactatgaatcaatggaaggggaggaagcagtacctttgcagtacgttggtttgttggatggagctgtaggtgtaggcttaggggtattaaacatggaattaggctcaaaaacagattggacaaaagcattattaatttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K302028_sequence/1
type: Sequence
persistent identity: BBa K302028 sequence
display id: BBa_K302028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatccttatatacacctacagattattatatgattcgggttcctttagtacatcaagacttaaaaaatgagaactctcaggatatcgatcagttattacatgacctttgcaacgattcattatttcgggaacaaatactggtatctagcaggacactatatgaaacaatacatactttcctgcaagcgccggataaattaaaagggaaaaaaaagcgcaactttcaacaggctattttgaagtatgcaacaagaagagcaacaagaaccacaccttttggccttttttcttcagtcggtatagggtcgttttctgataaaaatcacttatcttttaatcaacattcattttacaaaaaggctcgtgttgatttggagtggctctaccaattaattagaaaattggaaaacgaatacaccgaccgactttcttttacattaaattccgcttgttatattaagggtgaccgggcttacttgttgtacagcacagatggaaaatctgaagaagttagtgttcgtgcgacatctgttttctatttgataaatgaactgtgtggtgaatctgctgcatatcaagatataatccgttgtttgatagataactatccaaatactccaataaataaaattaatcagtacgtagcagaccttattgacaaagagttccttatatcaaacttacggccgccgatgactgtttcagatcaatttcagtatttaattgatcaagcggaaagccgccatattccaaatgaactcattcaggcttgtaaagacattcaatatcagatagatgcatataatcggatcactatcggagagggagagcatcagtatttaaatctgattgaaacaatgaataaactcataaaggcatcatctcctctgcaagtagacgctgggctggcagactcctccattcaattagataatgaaacatctctcgccataagtgaattggcaagcatgtttacttatatggcttctccctctgccaatacattagaccacttggaaaaataccacaatgtatttttggaacgctatggatatgaaagagaagttcctctcttagaaatgctatgttccagcactggcattggtgctcctgctacgtacacgaatcctgctaatgagttctttgaagaaacatcatttggggagcaattttcaccggaaatgaaacaatttttcatgagaaagtattttgaatcagttagaaagaaagctcctattcaattagacgacgaaacattccatagaatttgcaactctgaaattgctgatgaggaaattccattatcatttgaactcaatttctttgttaaattacgaaatgggagagttaagctttatttaggccccaacgtcggatctacccgcgcagggaaaacatttgggagattttctcatatgtctgattcaatcagtgaaatcataaagaccttacataacaaggagaaagagttaacagagtgtaatacgaaagtttgtgaactgagtattgtgcctaatcaaactaggtctggaaatgtaacaagaaatgtaagctaccgagaaaaagagatgtctctttttacgaacagtgctctgcatctcaatgattccgtcaaagctgaagatattctaattggaatcaataaagaccataacttttatgctagacataaaacaactggcgaaattctgtcttttgagtcaaatcatatgtttaatcctttattaatgaccaacgctgtacggtttttattagagatttcaagagatggtaaaagaaaatggaatgatttcccgtggtttagtatctatagtgatttcaagtatattcctgaaatcaaatataaagagataaccttatcttgtgaacaatggctgatatacaaaaatgatttaagcatgcactcgaatgcatcactagaagagataaaatctcctttttttgaatttcatcgtacttatgaactgccgcaaacattttatatcgttaacgcaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z