LOCUS       BBa_K302035             1006 bp    DNA     linear       25-Nov-2024
DEFINITION  Sucrose-limitation induced kill switch
ACCESSION   BBa_K302035
VERSION     BBa_K302035.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A -10
     promoter        1..97
                     /label=BBa_K143012
     stem_loop       146..211
                     /label=Sucrose binding loop
     promoter        106..288
                     /label=BBa_K302031
     RBS             297..316
                     /label=mazE native RBS
     misc_feature    317..565
                     /label=K302032
     misc_feature    565..903
                     /label=K302033
     misc_feature    297..903
                     /label=BBa_K302034
     stem_loop       919..938
                     /label=T7 TE
     misc_feature    945..945
                     /label=stop
     polyA_site      939..952
                     /label=polya
     misc_feature    912..952
                     /label=BBa_B0012
     terminator      912..952
                     /label=BBa_B0012
     stem_loop       973..995
                     /label=stem_loop
     misc_feature    961..1006
                     /label=BBa_B0011
     terminator      961..1006
                     /label=BBa_B0011
     terminator      912..1006
                     /label=BBa_B0014
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagaggaaaa gtaaatcgcg
      121 cgggtttgtt actgataaag caggcaagac ctaaaatgtg taaagggcaa agtgtatact
      181 ttggcgtcac cccttacata ttttaggtct ttttttattg tgcgtaacta acttgccatc
      241 ttcaaacagg agggctggaa gaagcagacc gctaacacag tacataaata ctagagagcg
      301 gtttgaggaa agggttatga tccacagtag cgtaaagcgt tggggaaatt caccggcggt
      361 gcggatcccg gctacgttaa tgcaggcgct caatctgaat attgatgatg aagtgaagat
      421 tgacctggtg gatggcaaat taattattga gccagtgcgt aaagagcccg tatttacgct
      481 tgctgaactg gtcaacgaca tcacgccgga aaacctccac gagaatatcg actggggaga
      541 gccgaaagat aaggaagtct ggtaatggta agccgatacg tacccgatat gggcgatctg
      601 atttgggttg attttgaccc gacaaaaggt agcgagcaag ctggacatcg tccagctgtt
      661 gtcctgagtc ctttcatgta caacaacaaa acaggtatgt gtctgtgtgt tccttgtaca
      721 acgcaatcaa aaggatatcc gttcgaagtt gttttatccg gtcaggaacg tgatggcgta
      781 gcgttagctg atcaggtaaa aagtatcgcc tggcgggcaa gaggagcaac gaagaaagga
      841 acagttgccc cagaggaatt acaactcatt aaagccaaaa ttaacgtact gattgggtaa
      901 taatactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
      961 agagaatata aaaagccaga ttattaatcc ggctttttta ttattt
//