LOCUS       BBa_K305009             1131 bp    DNA     linear       27-Dec-2024
DEFINITION  srfA promoter, short > GFP
ACCESSION   BBa_K305009
VERSION     BBa_K305009.1  
FEATURES             Location/Qualifiers
     promoter        1..247
                     /label=BBa_K305007
     RBS             256..268
                     /label=RBS-3\Weak
     RBS             262..265
                     /label=RBS
     misc_feature    256..268
                     /label=BBa_B0032
     RBS             256..268
                     /label=BBa_B0032
     CDS             275..994
                     /label=GFP protein
     CDS             275..994
                     /label=BBa_E0040
     stem_loop       1014..1057
                     /label=stem_loop
     misc_feature    1003..1082
                     /label=BBa_B0010
     terminator      1003..1082
                     /label=BBa_B0010
     stem_loop       1098..1117
                     /label=T7 TE
     misc_feature    1124..1124
                     /label=stop
     polyA_site      1118..1131
                     /label=polya
     misc_feature    1091..1131
                     /label=BBa_B0012
     terminator      1091..1131
                     /label=BBa_B0012
ORIGIN
        1 gattgaacgc agcagtttgg tttaaaaatt tttatttttc tgtaaataat gtttagtgga
       61 aatgattgcg gcatcccgca aaaaatattg ctgtaaataa actggaatct ttcggcatcc
      121 cgcatgaaac ttttcaccca tttttcggtg ataaaaacat ttttttcatt taaactgaac
      181 ggtagaaaga taaaaaatat tgaaaacaat gaataaatag ccaaaattgg tttcttatta
      241 gggtgggtac tagagtcaca caggaaagta ctagatgcgt aaaggagaag aacttttcac
      301 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      361 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      421 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca
      481 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
      541 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
      601 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
      661 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
      721 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
      781 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
      841 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
      901 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
      961 tacacatggc atggatgaac tatacaaata ataatactag agccaggcat caaataaaac
     1021 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1081 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//