LOCUS       BBa_K305010             1321 bp    DNA     linear       23-Nov-2024
DEFINITION  srfA promoter, long > GFP
ACCESSION   BBa_K305010
VERSION     BBa_K305010.1  
FEATURES             Location/Qualifiers
     promoter        1..437
                     /label=BBa_K305008
     RBS             446..458
                     /label=RBS-3\Weak
     RBS             452..455
                     /label=RBS
     misc_feature    446..458
                     /label=BBa_B0032
     RBS             446..458
                     /label=BBa_B0032
     CDS             465..1184
                     /label=GFP protein
     CDS             465..1184
                     /label=BBa_E0040
     stem_loop       1204..1247
                     /label=stem_loop
     misc_feature    1193..1272
                     /label=BBa_B0010
     terminator      1193..1272
                     /label=BBa_B0010
     stem_loop       1288..1307
                     /label=T7 TE
     misc_feature    1314..1314
                     /label=stop
     polyA_site      1308..1321
                     /label=polya
     misc_feature    1281..1321
                     /label=BBa_B0012
     terminator      1281..1321
                     /label=BBa_B0012
ORIGIN
        1 gattgaacgc agcagtttgg tttaaaaatt tttatttttc tgtaaataat gtttagtgga
       61 aatgattgcg gcatcccgca aaaaatattg ctgtaaataa actggaatct ttcggcatcc
      121 cgcatgaaac ttttcaccca tttttcggtg ataaaaacat ttttttcatt taaactgaac
      181 ggtagaaaga taaaaaatat tgaaaacaat gaataaatag ccaaaattgg tttcttatta
      241 gggtggggtc ttgcggtctt tatccgctta tgttaaacgc cgcaatgctg actgacggca
      301 gcctgcttta atagcggcca tctgtttttt gattggaagc actgcttttt aagtgtagta
      361 ctttgggcta tttcggctgt tagttcataa gaattaaaag ctgatatgga taagaaagag
      421 aaaatgcgtt gcacatgtac tagagtcaca caggaaagta ctagatgcgt aaaggagaag
      481 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      541 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
      601 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt
      661 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga
      721 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
      781 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
      841 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
      901 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
      961 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
     1021 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
     1081 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
     1141 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agccaggcat
     1201 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1261 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1321 a
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