BBa_K309032
1
BBa_K309032
pMec-7::eCFP::unc-54 3' UTR (for use in C. elegans chassis)
2010-10-26T11:00:00Z
2015-05-08T01:11:53Z
From C. elegans (pMec-7, unc-54 'UTR)and A. victoria (eCFP) DNA.
This is a construct consisting of a Mec-7 promoter, eCFP, and an unc-54 3'UTR. Worms transformed with this construct will emit light at 477 nm from the touch receptor neurons when excited with light at 434 nm.
false
false
_428_
0
6985
9
Not in stock
true
Parts PCRed out of genomic/plasmid DNA and ligated together using T4 ligase. A Kozak sequence was added in front of the reporter (eCFP) in order to facilitate the translation process.
false
Thai Phi
component2219864
1
BBa_K309012
component2219860
1
BBa_K309000
component2219863
1
BBa_K309020
annotation2219863
1
BBa_K309020
range2219863
1
730
1458
annotation2219860
1
BBa_K309000
range2219860
1
1
721
annotation2219864
1
BBa_K309012
range2219864
1
1467
2358
BBa_K309020
1
BBa_K309020
eCFP (for use in C. elegans chassis)
2010-10-21T11:00:00Z
2015-05-08T01:11:53Z
Genetic mutant of GFP (Green Fluorescent Protein) from Aequorea victoria. (pIC517).
This is a reporter for use in a C. elegans chassis. Excitation wavelength of 434 nm and emission wavelength of 477 nm.
false
false
_428_
0
6985
9
It's complicated
false
This part was PCRed out of a plasmid.
false
Thai Phi
annotation2108971
1
Kozak
range2108971
1
1
9
annotation2108982
1
Start Codon
range2108982
1
10
12
BBa_K309012
1
BBa_K309012
Unc-54 3' UTR (for use in C. elegans chassis)
2010-10-21T11:00:00Z
2015-05-08T01:11:53Z
From C. elegans genomic DNA.
This is a terminator for use in C. elegans chassis.
false
false
_428_
0
4458
9
It's complicated
false
The exact length and sequence of the functional sequence that acts as the promoter in C. elegans has not been elucidated. Thus this sequence is a section of genomic DNA that exists in behind of the Unc-54 gene that has been previously demonstrated to function as a promoter, with the same expression pattern as the Unc-54 gene.
false
Chris Palmer
BBa_K309000
1
BBa_K309000
pMec-7 (C. elegans promoter)
2010-10-21T11:00:00Z
2015-05-08T01:11:53Z
From C. elegans genomic DNA.
This is a promoter for use in C. elegans chassis. It specifically targets the mechanoreceptor neurons.
false
false
_428_
0
4458
9
It's complicated
false
The exact length and sequence of the functional sequence that acts as the promoter in C. elegans has not been elucidated. Thus this sequence is a section of genomic DNA that exists in front of the Mec-7 gene that has been previously demonstrated to function as a promoter, with the same expression pattern as the Mec-7 gene.
false
Chris Palmer
BBa_K309000_sequence
1
tgataattgccatttccttcgatttctgactttcaattgtgatatggataacctaaaatctgccattcaaaatgaaaactcttaaagtttaagaggctttcaacgaccccgcgactttcccacctcatcgtttttcccttctatcttattctaattttttgtattttgtgtcagtttgctatatcctcatcaccttttttaccgtttaatctctctatagtattgttcgggtggtttcaagatgaaacgttttgtgtgtagcatttttgaaacacagcggaaaaatgcagaaactatcatccacaatgcattaagtgcactcattgtgatgactcctaaccccctcgccccaccatttgtctcttcacaaattcatggcacaattcaaaaatggctttttgtgttggatcttgatctcgtctctttccttcttctttcgtttctctcggaccactcattattcgaatgccatgcatttgttgatgatgcgcacgatggtcgcacacactacaacagatatgatggtggctgtgcgtggtgcagagctcatgaataagtgatggggcaccgagatgtgactcctcccattattcgtcttgagtcgcatcttttgtcttggtccgttgtcagtcgagcctcaaggcagccgttccgccagtgatcgcctgctaagaagttgtaggtgtagaggaagaaacgtcgggtccaaatttcaagcaac
BBa_K309020_sequence
1
gccgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa
BBa_K309012_sequence
1
cattcgtagatttccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcagcatctcgcgcccgtgcctctgacttctaagtccaattactcttcaacatccctacatgctctttctccctgtgctcccaccccctatttttgttattatcaaaaaaacttcttcttaatttctttgttttttagcttcttttaagtcacctctaacaatgaaattgtgtagattcaaaaatagaattaattcgtaataaaaagtcgaaaaaaattgtgctccctccccccattaataataattctatcccaaaatctacacaatgttctgtgtacacttcttatgttttttttacttctgataaattttttttgaaacatcatagaaaaaaccgcacacaaaataccttatcatatgttacgtttcagtttatgaccgcaatttttatttcttcgcacgtctgggcctctcatgacgtcaaatcatgctcatcgtgaaaaagttttggagtatttttggaatttttcaatcaagtgaaagtttatgaaattaattttcctgcttttgctttttgggggtttcccctattgtttgtcaagagtttcgaggacggcgtttttcttgctaaaatcacaagtattgatgagcacgatgcaagaaagatcggaagaaggtttgggtttgaggctcagtggaaggtgagtagaagttgataatttgaaagtggagtagtgtctatggggtttttgccttaaatgacagaatacattcccaatataccaaacataactgtttcctacttgtcggccgtacggg
BBa_K309032_sequence
1
tgataattgccatttccttcgatttctgactttcaattgtgatatggataacctaaaatctgccattcaaaatgaaaactcttaaagtttaagaggctttcaacgaccccgcgactttcccacctcatcgtttttcccttctatcttattctaattttttgtattttgtgtcagtttgctatatcctcatcaccttttttaccgtttaatctctctatagtattgttcgggtggtttcaagatgaaacgttttgtgtgtagcatttttgaaacacagcggaaaaatgcagaaactatcatccacaatgcattaagtgcactcattgtgatgactcctaaccccctcgccccaccatttgtctcttcacaaattcatggcacaattcaaaaatggctttttgtgttggatcttgatctcgtctctttccttcttctttcgtttctctcggaccactcattattcgaatgccatgcatttgttgatgatgcgcacgatggtcgcacacactacaacagatatgatggtggctgtgcgtggtgcagagctcatgaataagtgatggggcaccgagatgtgactcctcccattattcgtcttgagtcgcatcttttgtcttggtccgttgtcagtcgagcctcaaggcagccgttccgccagtgatcgcctgctaagaagttgtaggtgtagaggaagaaacgtcgggtccaaatttcaagcaactactagaggccgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagagcattcgtagatttccaactgagcgccggtcgctaccattaccaacttgtctggtgtcaaaaataataggggccgctgtcatcagagtaagtttaaactgagttctactaactaacgagtaatatttaaattttcagcatctcgcgcccgtgcctctgacttctaagtccaattactcttcaacatccctacatgctctttctccctgtgctcccaccccctatttttgttattatcaaaaaaacttcttcttaatttctttgttttttagcttcttttaagtcacctctaacaatgaaattgtgtagattcaaaaatagaattaattcgtaataaaaagtcgaaaaaaattgtgctccctccccccattaataataattctatcccaaaatctacacaatgttctgtgtacacttcttatgttttttttacttctgataaattttttttgaaacatcatagaaaaaaccgcacacaaaataccttatcatatgttacgtttcagtttatgaccgcaatttttatttcttcgcacgtctgggcctctcatgacgtcaaatcatgctcatcgtgaaaaagttttggagtatttttggaatttttcaatcaagtgaaagtttatgaaattaattttcctgcttttgctttttgggggtttcccctattgtttgtcaagagtttcgaggacggcgtttttcttgctaaaatcacaagtattgatgagcacgatgcaagaaagatcggaagaaggtttgggtttgaggctcagtggaaggtgagtagaagttgataatttgaaagtggagtagtgtctatggggtttttgccttaaatgacagaatacattcccaatataccaaacataactgtttcctacttgtcggccgtacggg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z