BBa_K310002
1
BBa_K310002
MicF (sRNA)
2010-09-14T11:00:00Z
2015-05-08T01:11:53Z
Escherichia coli MG1655
micF is a small non-coding RNA regulator that binds to the 5' UTR of OmpF via non-perfect base pairing. The primary action of micA is the translational repression of OmpF by ribosome occlusion of OmpF mRNA and subsequent destabalization of the transcript. micF binds RNA chaperone, hfq, which works to stabalize micA in the cytoplasm, accelerate sRNA-mRNA base pair annealing, recruit the RNA degradosome to the sRNA-mRNA complex.
false
false
_429_
0
4552
9
It's complicated
true
8 nucleotides have been added to the 5' of micF (scar coding)
false
Francis Lee
BBa_K310026
1
BBa_K310026
Ptet + MicF
2010-10-26T11:00:00Z
2015-05-08T01:11:54Z
e. coli k12 mg1655
coming soon
false
false
_429_
0
6020
9
Not in stock
false
coming soon
false
Erin Borchardt
component2112003
1
BBa_K310002
component2111998
1
BBa_R0040
annotation2111998
1
BBa_R0040
range2111998
1
1
54
annotation2112003
1
BBa_K310002
range2112003
1
63
195
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986785
1
-35
range1986785
1
20
25
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986787
1
-10
range1986787
1
43
48
annotation1986786
1
TetR 2
range1986786
1
26
44
BBa_K310026_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagaggctatcatcattaactttatttattaccgtcattcatttctgaatgtctgtttacccctatttcaaccggatgcctcgcattcggttttttttacccttctttacacacttttcattattctgtgctaccaca
BBa_K310002_sequence
1
gctatcatcattaactttatttattaccgtcattcatttctgaatgtctgtttacccctatttcaaccggatgcctcgcattcggttttttttacccttctttacacacttttcattattctgtgctaccaca
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z