BBa_K311002
1
BBa_K311002
Constitutive lac promoter with downstream EGFP
2010-10-14T11:00:00Z
2015-05-08T01:11:54Z
genomic DNA
The mutations were generated to get a constitutive lac promoter. The promoter was cloned in pSB1C3 upstream to the EGFP. The promoter activity was checked by transforming the recombinant plasmid in Lac repressor negative (TOP10) and Lac repressor positive (DH5 alpha pro) E. coli cells. The recombinant cells were grown in the presence of varying concentration of inducer (IPTG) and also in the absence of the inducer. The culture was collected at different time points post induction and promoter activity was measured by measuring the fluorescence intensity under FACs. Both the cell types show constitutive behavior of the promoter.
false
false
_430_
0
7095
9
It's complicated
false
The lac and EGFP were obtained from one of our home made plasmids by digesting it with EcoR I and Pst I. The part was ligated to EcoR I and Pst I cut pSB1C3.
false
Ethan Johnson, Claudia Schmidt-Dannert, Poonam Srivastava, Ian Windsor
annotation2086646
1
RBS-3\Weak
range2086646
1
1
13
annotation2086650
1
BBa_E0040
range2086650
1
20
739
annotation2086648
1
RBS
range2086648
1
7
10
annotation2086651
1
BBa_B0016
range2086651
1
748
795
annotation2086649
1
GFP protein
range2086649
1
20
739
annotation2086647
1
BBa_B0032
range2086647
1
1
13
BBa_K311002_sequence
1
tgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcatcctgaacttatctagacccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctggatccatatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaaatgcatccatggcggccgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z