BBa_K311003 1 BBa_K311003 Bacterial Microcompartment Proteins 2010-10-14T11:00:00Z 2015-05-08T01:11:54Z Salmonella enterica LT2 genomic DNA Three proteins subunits of a bacterial micro-compartment. The genes are in the pSB1C3 plasmid. Three total genes are present, two are together in a single operon and the single gene is upstream from the pair. They are flanked directly upstream by a mutated Lac promoter to make them constitutive. The genes were amplified by PCR from genomic DNA using primers that would introduce restriction sites flanking the genes. false false _430_ 0 7095 9 It's complicated false The genes were amplified by PCR from genomic DNA using primers that would introduce restriction sites flanking the genes. The genes were ligated into a modified pUC biobrick vector containing a constitutive promoter. The genes were taking in their native operons and ligated sequential according to size. false Matthew Adams, Swati Choudhary, Anthony Goering, Rachel Farr, Ethan Johnson, Annie Kathuria, Poonam Srivastava, Ian Windsor annotation2099773 1 BBa_E0040 range2099773 1 20 739 annotation2099774 1 BBa_B0016 range2099774 1 748 795 annotation2099770 1 BBa_B0032 range2099770 1 1 13 annotation2099772 1 GFP protein range2099772 1 20 739 annotation2099771 1 RBS range2099771 1 7 10 BBa_K311003_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgaataaagaacgcattattcaggaatttgtgccgggcaaacaggtcacgctggcgcatctcattgcgcaccctggtgaagaactggcgaaaaagatcggcgttcccgatgctggcgcgattggcattatgactttaacgccgggggaaacggcgatgatcgctggcgatttagcgatgaaggctgcggatgtacacatcggttttctcgatagatttagcggcgcgctggtgatttatggcaccgtcggcgcagtagaagaagcgctattacagacggtcagcgggctggggcgattattaaactttaccctgtgtgagttaacaaaaagttaagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatgtactagacccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatggaagcattaggaatgattgaaacccggggcctggttgcgctgattgaggcctccgatgcgatggtaaaagccgcgcgcgtgaagctggtcggcgtgaagcagattggcggtggcctgtgtactgccatggtgcgtggcgatgtggcggcgtgcaaagccgcaaccgatgctggcgccgctgcggcgcagcgcattggcgagttggtctccgtacacgtgattccacgcccgcacggcgatctggaagaagtgttcccgatcagcttcaaaggcgacagcaacatttgatggtaggccggataagacgcgcagcgtcgccatccggcatgaatgcgcgccattgcctgatggtgcgcagattcaaggcctacgacccaacgggcaatccatggaggcggatatgaaactggcagtcgtcacaggacaaatcgtatgtaccgtccgccatcagggactggcgcatgacaaattgctgatggtggaaatgatcgatgcccagggcaaccccgacgggcagtgtgccgtcgctatcgacagtatcggggcgggaaccggagagtgggtactgctggtcagcggcagttccgcacgccaggcgcatcgcagcgaattatcaccggtcgatctgtgcgtcattggcatcgtcgatgaagtggtggctggcgggaaagtggttttccataaatag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z