BBa_K311004 1 BBa_K311004 Complement of Ethanolamine Utilization Microcompartment proteins from Salmonella LT2 2010-10-14T11:00:00Z 2015-05-08T01:11:54Z Salmonella enterica LT2 genomic DNA Three proteins subunits of a bacterial micro-compartment. The genes are in the pSB1C3 plasmid. A total of 5 micro-compartment genes are cloned in a single plasmid. They are arranged as two sets of two genes (in a single operon) with one gene which is upstream to the pairs. They are flanked directly upstream by a mutated Lac promoter to make them constitutively expressed. The genes were amplified by PCR from genomic DNA using primers that would introduce restriction sites flanking the genes. false false _430_ 0 7095 9 In stock true The genes were amplified by PCR from genomic DNA using primers that would introduce restriction sites flanking the genes. The genes were ligated into a modified pUC biobrick vector containing a constitutive promoter. The genes were taking in their native operons and ligated sequential according to size. false Matthew Adams, Swati Choudhary, Anthony Goering, Rachel Farr, Ethan Johnson, Annie Kathuria, Poonam Srivastava, Ian Windsor annotation2099761 1 modified plac range2099761 1 556 679 annotation2099767 1 BBa_K311004 range2099767 1 1 2771 annotation2099760 1 Eut S range2099760 1 140 475 annotation2099766 1 EutK range2099766 1 2277 2771 annotation2099763 1 EutN range2099763 1 1086 1385 annotation2099762 1 EutM range2099762 1 695 985 annotation2099765 1 EutL range2099765 1 1605 2264 annotation2099759 1 modified plac range2099759 1 1 124 annotation2099764 1 modified plac range2099764 1 1466 1589 BBa_K311004_sequence 1 cccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgaataaagaacgcattattcaggaatttgtgccgggcaaacaggtcacgctggcgcatctcattgcgcaccctggtgaagaactggcgaaaaagatcggcgttcccgatgctggcgcgattggcattatgactttaacgccgggggaaacggcgatgatcgctggcgatttagcgatgaaggctgcggatgtacacatcggttttctcgatagatttagcggcgcgctggtgatttatggcaccgtcggcgcagtagaagaagcgctattacagacggtcagcgggctggggcgattattaaactttaccctgtgtgagttaacaaaaagttaagcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatgtactagacccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatggaagcattaggaatgattgaaacccggggcctggttgcgctgattgaggcctccgatgcgatggtaaaagccgcgcgcgtgaagctggtcggcgtgaagcagattggcggtggcctgtgtactgccatggtgcgtggcgatgtggcggcgtgcaaagccgcaaccgatgctggcgccgctgcggcgcagcgcattggcgagttggtctccgtacacgtgattccacgcccgcacggcgatctggaagaagtgttcccgatcagcttcaaaggcgacagcaacatttgatggtaggccggataagacgcgcagcgtcgccatccggcatgaatgcgcgccattgcctgatggtgcgcagattcaaggcctacgacccaacgggcaatccatggaggcggatatgaaactggcagtcgtcacaggacaaatcgtatgtaccgtccgccatcagggactggcgcatgacaaattgctgatggtggaaatgatcgatgcccagggcaaccccgacgggcagtgtgccgtcgctatcgacagtatcggggcgggaaccggagagtgggtactgctggtcagcggcagttccgcacgccaggcgcatcgcagcgaattatcaccggtcgatctgtgcgtcattggcatcgtcgatgaagtggtggctggcgggaaagtggttttccataaataggcggccgcctcgagcatcaccatcaccatcactgattctccttacgcatctgtgcggtatttcacaccgcatgtactagacccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgtctagtagaaggaggagatctatgcctgcattagatttaattcgaccttcagtgactgccatgcgcgtgattgcctccgtgaatgacggctttgcgcgggaacttaaattaccgccacatatacgtagtctcggactcatcacggcagattctgatgatgtgacctatattgccgctgacgaagcgacaaaacaggcgatggtggaagtggtctatggccgctccctctatgccggggcggctcacgggccgtcgcctaccgctggtgaggtgttgattatgctgggcggaccgaacccggcggaagtgcgcgccggtctggatgcaatggtcgccagcattgaaaacggcgcggcgttccagtgggcgaacgatgcggaaaataccgcattcctggcgcatgtggtttcgcgtaccggctcgtacctctcgtctaccgcaggtatcgcgctgggcgatccaatggcctacctggtggcgccgccgctggaagcgacattcggcattgacgcggcgatgaaatccgctgacgttcagctggtgacttacgtgccgccgccgtcggaaaccaactattcggccgcgttcttaacgggaagccaggccgcgtgtaaagccgcctgcaacgcctttaccgacgccgtgctggatatcgcccgtaaccctgtccagcgtgcgtaacggaggctgccaatgatcaatgccctgggattactggaagtggacggaatggtcgccgccgtcgatgcggcggatgctatgctgaaagcggccaacgtgcgtttgctcagtcatcaagtgctcgaccctggcaggctcacgctggtggtggagggcgatctggcggcgtgtcgtgcggcgctggatgccggaagcgctgccgcgcagcgcacaggccgtgtgattagtcgcaaagaaattggtcgtccggaagaggacacccagtggctgattggcgggtttgcgcgtgcgacaacgccgactgagaaagcaccccaggtacccgcaacgcctgagttcgctgaggcgttattggcgctgctggcatcagtacgccagggaatgacggcgggagaggttgccgcgcattttggctggccgctggagcaggccagaaacgtgctggaacaactcttttctgacggagcgttgcgtaaacgcagtagtcgctatcgcatcaaaaattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z