LOCUS       BBa_K313009             1153 bp    DNA     linear       22-Nov-2024
DEFINITION  cre recombinase assay device
ACCESSION   BBa_K313009
VERSION     BBa_K313009.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    55..88
                     /label=BBa_I718016
     stem_loop       108..151
                     /label=stem_loop
     misc_feature    97..176
                     /label=BBa_B0010
     terminator      97..176
                     /label=BBa_B0010
     stem_loop       192..211
                     /label=T7 TE
     misc_feature    218..218
                     /label=stop
     polyA_site      212..225
                     /label=polya
     misc_feature    185..225
                     /label=BBa_B0012
     terminator      185..225
                     /label=BBa_B0012
     terminator      97..225
                     /label=BBa_B0015
     misc_feature    234..267
                     /label=BBa_I718017
     RBS             276..290
                     /label=RBS-1\Strong
     RBS             283..287
                     /label=RBS
     misc_feature    276..290
                     /label=BBa_B0030
     RBS             276..290
                     /label=BBa_B0030
     CDS             297..1016
                     /label=GFP protein
     CDS             297..1016
                     /label=BBa_E0040
     stem_loop       1036..1079
                     /label=stem_loop
     misc_feature    1025..1104
                     /label=BBa_B0010
     terminator      1025..1104
                     /label=BBa_B0010
     stem_loop       1120..1139
                     /label=T7 TE
     misc_feature    1146..1146
                     /label=stop
     polyA_site      1140..1153
                     /label=polya
     misc_feature    1113..1153
                     /label=BBa_B0012
     terminator      1113..1153
                     /label=BBa_B0012
     misc_feature    276..1153
                     /label=BBa_E0840
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagataact
       61 tggtatagca tacattatac gaacggtata ctagagccag gcatcaaata aaacgaaagg
      121 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      181 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtaccgtt
      241 cgtatacgat acattatacg aagttattac tagagattaa agaggagaaa tactagatgc
      301 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
      361 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
      421 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
      481 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg
      541 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
      601 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
      661 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
      721 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
      781 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
      841 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
      901 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
      961 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact
     1021 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1081 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1141 ttctgcgttt ata
//