LOCUS       BBa_K313016             1457 bp    DNA     linear       10-May-2024
DEFINITION  cre recombinase assay device
ACCESSION   BBa_K313016
VERSION     BBa_K313016.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    55..88
                     /label=BBa_I718017
     CDS             97..1287
                     /label=tetA(C) backwards
     misc_feature    97..99
                     /label=stop
     primer_bind     97..116
                     /label=Rev primer J31006
     primer_bind     1264..1287
                     /label=Fwd primer J31006
     CDS             97..1287
                     /label=BBa_J31006
     RBS             1296..1310
                     /label=Reverse RBS
     RBS             1296..1310
                     /label=BBa_J44001
     misc_feature    1319..1354
                     /label=BBa_K313012
     stem_loop       1370..1389
                     /label=T7 TE
     misc_feature    1396..1396
                     /label=stop
     polyA_site      1390..1403
                     /label=polya
     misc_feature    1363..1403
                     /label=BBa_B0012
     terminator      1363..1403
                     /label=BBa_B0012
     stem_loop       1424..1446
                     /label=stem_loop
     misc_feature    1412..1457
                     /label=BBa_B0011
     terminator      1412..1457
                     /label=BBa_B0011
     terminator      1363..1457
                     /label=BBa_B0014
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtaccgt
       61 tcgtatacga tacattatac gaagttatta ctagagttag gtcgaggtgg cccggctcca
      121 tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc
      181 caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccaat
      241 gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc
      301 atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag
      361 aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc
      421 cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc
      481 ggggccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag
      541 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc
      601 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt
      661 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg
      721 acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt
      781 gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc
      841 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
      901 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
      961 ggtgatgccg gccacgatgc gtccggcgta gaggatccac aggacgggtg tggtcgccat
     1021 gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa
     1081 gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc
     1141 tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc
     1201 cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat
     1261 gacgatgagc gcattgttag atttcattac tagagtttct cctctttaat tactagagag
     1321 taccgttcgt ataatgtatg ctataccaag ttattactag agtcacactg gctcaccttc
     1381 gggtgggcct ttctgcgttt atatactaga gagagaatat aaaaagccag attattaatc
     1441 cggctttttt attattt
//