BBa_K313012
1
BBa_K313012
lox66(reverse)
2010-10-21T11:00:00Z
2015-05-08T01:11:54Z
It was synthesized in oligonucleotide form.
This is a recombination site for cre recombinase. Please note that it is in reverse direction.
false
false
_433_
0
5929
9
It's complicated
false
nothing special
false
Ryo Kariyazono, Ryosuke Kamei
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
BBa_I712074
1
BBa_I712074
T7 promoter (strong promoter from T7 bacteriophage)
2007-10-21T11:00:00Z
2015-08-31T04:07:46Z
T7 bacteriophage
T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
false
false
_130_
0
1856
9
In stock
false
true
Rok Gaber
BBa_I718017
1
lox71
lox71
2007-10-25T11:00:00Z
2015-08-31T04:07:52Z
This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated.
Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI)
You can follow the construction process by following the links available in the Paris iGEM 2007 wiki:
http://parts.mit.edu/igem07/index.php/Paris
"freezer" section
plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed
Released HQ 2013
Lox71 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularly in mouse genetics.
lox site recombination is catalysed by a Site specific recombinase, Cre.
lox sequences are composed of an 8 bp Core sequence surrounded by two Arms.
The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm.
As a consequence, after a recombination between a lox71 and a lox66 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox71 & lox66 sites is potentially interesting when the recombination reaction must be "irreversible".
false
false
_141_
0
1568
9
In stock
false
No modifaication was made on lox71 sequence
true
Eimad Shotar
BBa_J44001
1
BBa_J44001
Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030
2006-08-01T11:00:00Z
2015-08-31T04:08:48Z
Cloned from synthetic oligos
Released HQ 2013
Reverse version of RBS BBa_B0030
false
false
_61_71_
0
606
61
In stock
true
Repeats in oligos caused unusual products during cloning
true
Todd Eckdahl
annotation1893199
1
Reverse RBS
range1893199
1
1
15
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939303
1
BBa_B0012
component939311
1
BBa_B0011
annotation939303
1
BBa_B0012
range939303
1
1
41
annotation939311
1
BBa_B0011
range939311
1
50
95
BBa_J31006
1
tetA(C)b
tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
2006-07-11T11:00:00Z
2015-08-31T04:08:45Z
PsB1AT3
This is the coding region for Tetracyline resistance in the reverse orientation. If a promoter adn RBS are placed downstream and the entire region, from promoter to stop codon is flipped the cell will express tetracycline resistance.
false
false
_61_
0
919
61
It's complicated
true
It was cloned into PsB1A2 plasmids.
true
Sabriya Rosemond, Erin Zwack
annotation1884998
1
tetA(C) backwards
range1884998
1
1
1191
annotation1910576
1
Fwd primer J31006
range1910576
1
1168
1191
annotation1910575
1
Rev primer J31006
range1910575
1
1
20
annotation1910574
1
stop
range1910574
1
1
3
BBa_K313016
1
BBa_K313016
cre recombinase assay device
2010-10-21T11:00:00Z
2015-05-08T01:11:54Z
BBa_I712074, BBa_I718017, BBa_J31006, BBa_J44001, BBa_K313012, BBa_B0014
lorem ipsum
false
false
_433_
0
5929
9
It's complicated
false
nothing special
false
Ryosuke Kamei, Mami Nakatake, Kosuke Uekusa, Syota Suenami, Ryo Kariyazono
component2107568
1
BBa_K313012
component2107575
1
BBa_B0014
component2107565
1
BBa_J31006
component2107567
1
BBa_J44001
component2107560
1
BBa_I718017
component2107559
1
BBa_I712074
annotation2107575
1
BBa_B0014
range2107575
1
1363
1457
annotation2107568
1
BBa_K313012
range2107568
1
1319
1354
annotation2107567
1
BBa_J44001
range2107567
1
1296
1310
annotation2107559
1
BBa_I712074
range2107559
1
1
46
annotation2107560
1
BBa_I718017
range2107560
1
55
88
annotation2107565
1
BBa_J31006
range2107565
1
97
1287
BBa_J31006_sequence
1
ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcat
BBa_I718017_sequence
1
taccgttcgtatacgatacattatacgaagttat
BBa_K313016_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgcatactagagtaccgttcgtatacgatacattatacgaagttattactagagttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagtttctcctctttaattactagagagtaccgttcgtataatgtatgctataccaagttattactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_J44001_sequence
1
tttctcctctttaat
BBa_I712074_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgca
BBa_K313012_sequence
1
agtaccgttcgtataatgtatgctataccaagttat
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z