LOCUS       BBa_K313017             1262 bp    DNA     linear       10-May-2024
DEFINITION  terminator leakiness assay device
ACCESSION   BBa_K313017
VERSION     BBa_K313017.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    55..93
                     /label=B1006
     stem_loop       64..85
                     /label=modified thr terminator
     polyA_site      86..93
                     /label=PolyA
     polyA_site      55..63
                     /label=PolyA
     terminator      55..93
                     /label=BBa_B1006
     RBS             102..116
                     /label=RBS-1\Strong
     RBS             109..113
                     /label=RBS
     misc_feature    102..116
                     /label=BBa_B0030
     RBS             102..116
                     /label=BBa_B0030
     CDS             123..1159
                     /label=BBa_J61047
     stem_loop       1175..1194
                     /label=T7 TE
     misc_feature    1201..1201
                     /label=stop
     polyA_site      1195..1208
                     /label=polya
     misc_feature    1168..1208
                     /label=BBa_B0012
     terminator      1168..1208
                     /label=BBa_B0012
     stem_loop       1229..1251
                     /label=stem_loop
     misc_feature    1217..1262
                     /label=BBa_B0011
     terminator      1217..1262
                     /label=BBa_B0011
     terminator      1168..1262
                     /label=BBa_B0014
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaaaaa
       61 aaaccccgcc cctgacaggg cggggttttt ttttactaga gattaaagag gagaaatact
      121 agatgtccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga
      181 gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc
      241 atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata
      301 accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg
      361 cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc
      421 gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga
      481 tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca
      541 ctgatttcga ccaggttcgt tcactcatgg aaaatagcga tcgctgccag gatatacgta
      601 atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga
      661 tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa
      721 cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac
      781 tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt
      841 gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg
      901 ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag gatgactctg
      961 gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg cgagatatgg
     1021 cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc aatgtaaata
     1081 ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg cgcctgctgg
     1141 aagatggcga ttaagaattt actagagtca cactggctca ccttcgggtg ggcctttctg
     1201 cgtttatata ctagagagag aatataaaaa gccagattat taatccggct tttttattat
     1261 tt
//