LOCUS       BBa_K313018             1309 bp    DNA     linear       26-Dec-2024
DEFINITION  terminator leakiness assay device
ACCESSION   BBa_K313018
VERSION     BBa_K313018.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    55..93
                     /label=B1006
     stem_loop       64..85
                     /label=modified thr terminator
     polyA_site      86..93
                     /label=PolyA
     polyA_site      55..63
                     /label=PolyA
     terminator      55..93
                     /label=BBa_B1006
     misc_feature    102..140
                     /label=B1006
     stem_loop       111..132
                     /label=modified thr terminator
     polyA_site      133..140
                     /label=PolyA
     polyA_site      102..110
                     /label=PolyA
     terminator      102..140
                     /label=BBa_B1006
     RBS             149..163
                     /label=RBS-1\Strong
     RBS             156..160
                     /label=RBS
     misc_feature    149..163
                     /label=BBa_B0030
     RBS             149..163
                     /label=BBa_B0030
     CDS             170..1206
                     /label=BBa_J61047
     stem_loop       1222..1241
                     /label=T7 TE
     misc_feature    1248..1248
                     /label=stop
     polyA_site      1242..1255
                     /label=polya
     misc_feature    1215..1255
                     /label=BBa_B0012
     terminator      1215..1255
                     /label=BBa_B0012
     stem_loop       1276..1298
                     /label=stem_loop
     misc_feature    1264..1309
                     /label=BBa_B0011
     terminator      1264..1309
                     /label=BBa_B0011
     terminator      1215..1309
                     /label=BBa_B0014
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaaaaa
       61 aaaccccgcc cctgacaggg cggggttttt ttttactaga gaaaaaaaaa ccccgcccct
      121 gacagggcgg ggtttttttt tactagagat taaagaggag aaatactaga tgtccaattt
      181 actgaccgta caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg
      241 caagaacctg atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat
      301 gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt
      361 tcccgcagaa cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc
      421 agtaaaaact atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtccgggct
      481 gccacgacca agtgacagca atgctgtttc actggttatg cggcggatcc gaaaagaaaa
      541 cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg atttcgacca
      601 ggttcgttca ctcatggaaa atagcgatcg ctgccaggat atacgtaatc tggcatttct
      661 ggggattgct tataacaccc tgttacgtat agccgaaatt gccaggatca gggttaaaga
      721 tatctcacgt actgacggtg ggagaatgtt aatccatatt ggcagaacga aaacgctggt
      781 tagcaccgca ggtgtagaga aggcacttag cctgggggta actaaactgg tcgagcgatg
      841 gatttccgtc tctggtgtag ctgatgatcc gaataactac ctgttttgcc gggtcagaaa
      901 aaatggtgtt gccgcgccat ctgccaccag ccagctatca actcgcgccc tggaagggat
      961 ttttgaagca actcatcgat tgatttacgg cgctaaggat gactctggtc agagatacct
     1021 ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga gatatggccc gcgctggagt
     1081 ttcaataccg gagatcatgc aagctggtgg ctggaccaat gtaaatattg tcatgaacta
     1141 tatccgtaac ctggatagtg aaacaggggc aatggtgcgc ctgctggaag atggcgatta
     1201 agaatttact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
     1261 gagagagaat ataaaaagcc agattattaa tccggctttt ttattattt
//