LOCUS       BBa_K313019             1318 bp    DNA     linear       29-Nov-2024
DEFINITION  terminator leakiness assay device
ACCESSION   BBa_K313019
VERSION     BBa_K313019.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     stem_loop       62..81
                     /label=T7 TE
     misc_feature    88..88
                     /label=stop
     polyA_site      82..95
                     /label=polya
     misc_feature    55..95
                     /label=BBa_B0012
     terminator      55..95
                     /label=BBa_B0012
     stem_loop       116..138
                     /label=stem_loop
     misc_feature    104..149
                     /label=BBa_B0011
     terminator      104..149
                     /label=BBa_B0011
     terminator      55..149
                     /label=BBa_B0014
     RBS             158..172
                     /label=RBS-1\Strong
     RBS             165..169
                     /label=RBS
     misc_feature    158..172
                     /label=BBa_B0030
     RBS             158..172
                     /label=BBa_B0030
     CDS             179..1215
                     /label=BBa_J61047
     stem_loop       1231..1250
                     /label=T7 TE
     misc_feature    1257..1257
                     /label=stop
     polyA_site      1251..1264
                     /label=polya
     misc_feature    1224..1264
                     /label=BBa_B0012
     terminator      1224..1264
                     /label=BBa_B0012
     stem_loop       1285..1307
                     /label=stem_loop
     misc_feature    1273..1318
                     /label=BBa_B0011
     terminator      1273..1318
                     /label=BBa_B0011
     terminator      1224..1318
                     /label=BBa_B0014
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtcacac
       61 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc
      121 agattattaa tccggctttt ttattatttt actagagatt aaagaggaga aatactagat
      181 gtccaattta ctgaccgtac accaaaattt gcctgcatta ccggtcgatg caacgagtga
      241 tgaggttcgc aagaacctga tggacatgtt cagggatcgc caggcgtttt ctgagcatac
      301 ctggaaaatg cttctgtccg tttgccggtc gtgggcggca tggtgcaagt tgaataaccg
      361 gaaatggttt cccgcagaac ctgaagatgt tcgcgattat cttctatatc ttcaggcgcg
      421 cggtctggca gtaaaaacta tccagcaaca tttgggccag ctaaacatgc ttcatcgtcg
      481 gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca ctggttatgc ggcggatccg
      541 aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct ctagcgttcg aacgcactga
      601 tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc tgccaggata tacgtaatct
      661 ggcatttctg gggattgctt ataacaccct gttacgtata gccgaaattg ccaggatcag
      721 ggttaaagat atctcacgta ctgacggtgg gagaatgtta atccatattg gcagaacgaa
      781 aacgctggtt agcaccgcag gtgtagagaa ggcacttagc ctgggggtaa ctaaactggt
      841 cgagcgatgg atttccgtct ctggtgtagc tgatgatccg aataactacc tgttttgccg
      901 ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc cagctatcaa ctcgcgccct
      961 ggaagggatt tttgaagcaa ctcatcgatt gatttacggc gctaaggatg actctggtca
     1021 gagatacctg gcctggtctg gacacagtgc ccgtgtcgga gccgcgcgag atatggcccg
     1081 cgctggagtt tcaataccgg agatcatgca agctggtggc tggaccaatg taaatattgt
     1141 catgaactat atccgtaacc tggatagtga aacaggggca atggtgcgcc tgctggaaga
     1201 tggcgattaa gaatttacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1261 tatatactag agagagaata taaaaagcca gattattaat ccggcttttt tattattt
//