LOCUS       BBa_K313020             1421 bp    DNA     linear       20-Nov-2024
DEFINITION  terminator leakiness assay device
ACCESSION   BBa_K313020
VERSION     BBa_K313020.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     stem_loop       62..81
                     /label=T7 TE
     misc_feature    88..88
                     /label=stop
     polyA_site      82..95
                     /label=polya
     misc_feature    55..95
                     /label=BBa_B0012
     terminator      55..95
                     /label=BBa_B0012
     stem_loop       116..138
                     /label=stem_loop
     misc_feature    104..149
                     /label=BBa_B0011
     terminator      104..149
                     /label=BBa_B0011
     terminator      55..149
                     /label=BBa_B0014
     stem_loop       165..184
                     /label=T7 TE
     misc_feature    191..191
                     /label=stop
     polyA_site      185..198
                     /label=polya
     misc_feature    158..198
                     /label=BBa_B0012
     terminator      158..198
                     /label=BBa_B0012
     stem_loop       219..241
                     /label=stem_loop
     misc_feature    207..252
                     /label=BBa_B0011
     terminator      207..252
                     /label=BBa_B0011
     terminator      158..252
                     /label=BBa_B0014
     RBS             261..275
                     /label=RBS-1\Strong
     RBS             268..272
                     /label=RBS
     misc_feature    261..275
                     /label=BBa_B0030
     RBS             261..275
                     /label=BBa_B0030
     CDS             282..1318
                     /label=BBa_J61047
     stem_loop       1334..1353
                     /label=T7 TE
     misc_feature    1360..1360
                     /label=stop
     polyA_site      1354..1367
                     /label=polya
     misc_feature    1327..1367
                     /label=BBa_B0012
     terminator      1327..1367
                     /label=BBa_B0012
     stem_loop       1388..1410
                     /label=stem_loop
     misc_feature    1376..1421
                     /label=BBa_B0011
     terminator      1376..1421
                     /label=BBa_B0011
     terminator      1327..1421
                     /label=BBa_B0014
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtcacac
       61 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc
      121 agattattaa tccggctttt ttattatttt actagagtca cactggctca ccttcgggtg
      181 ggcctttctg cgtttatata ctagagagag aatataaaaa gccagattat taatccggct
      241 tttttattat tttactagag attaaagagg agaaatacta gatgtccaat ttactgaccg
      301 tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc
      361 tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa atgcttctgt
      421 ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag
      481 aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa
      541 ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac
      601 caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg
      661 ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt
      721 cactcatgga aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg
      781 cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac
      841 gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg
      901 caggtgtaga gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg
      961 tctctggtgt agctgatgat ccgaataact acctgttttg ccgggtcaga aaaaatggtg
     1021 ttgccgcgcc atctgccacc agccagctat caactcgcgc cctggaaggg atttttgaag
     1081 caactcatcg attgatttac ggcgctaagg atgactctgg tcagagatac ctggcctggt
     1141 ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga gtttcaatac
     1201 cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac tatatccgta
     1261 acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat taagaattta
     1321 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagagaga
     1381 atataaaaag ccagattatt aatccggctt ttttattatt t
//