LOCUS       BBa_K313021             1263 bp    DNA     linear       26-Dec-2024
DEFINITION  terminator leakiness assay device
ACCESSION   BBa_K313021
VERSION     BBa_K313021.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    55..94
                     /label=B1007
     stem_loop       64..85
                     /label=modified thr
     polyA_site      58..63
                     /label=PolyA
     polyA_site      86..91
                     /label=PolyA
     terminator      55..94
                     /label=BBa_B1007
     RBS             103..117
                     /label=RBS-1\Strong
     RBS             110..114
                     /label=RBS
     misc_feature    103..117
                     /label=BBa_B0030
     RBS             103..117
                     /label=BBa_B0030
     CDS             124..1160
                     /label=BBa_J61047
     stem_loop       1176..1195
                     /label=T7 TE
     misc_feature    1202..1202
                     /label=stop
     polyA_site      1196..1209
                     /label=polya
     misc_feature    1169..1209
                     /label=BBa_B0012
     terminator      1169..1209
                     /label=BBa_B0012
     stem_loop       1230..1252
                     /label=stem_loop
     misc_feature    1218..1263
                     /label=BBa_B0011
     terminator      1218..1263
                     /label=BBa_B0011
     terminator      1169..1263
                     /label=BBa_B0014
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagcgcaaa
       61 aaaccccgcc cctgacaggg cggggttttt tcgctactag agattaaaga ggagaaatac
      121 tagatgtcca atttactgac cgtacaccaa aatttgcctg cattaccggt cgatgcaacg
      181 agtgatgagg ttcgcaagaa cctgatggac atgttcaggg atcgccaggc gttttctgag
      241 catacctgga aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg caagttgaat
      301 aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg attatcttct atatcttcag
      361 gcgcgcggtc tggcagtaaa aactatccag caacatttgg gccagctaaa catgcttcat
      421 cgtcggtccg ggctgccacg accaagtgac agcaatgctg tttcactggt tatgcggcgg
      481 atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc
      541 actgatttcg accaggttcg ttcactcatg gaaaatagcg atcgctgcca ggatatacgt
      601 aatctggcat ttctggggat tgcttataac accctgttac gtatagccga aattgccagg
      661 atcagggtta aagatatctc acgtactgac ggtgggagaa tgttaatcca tattggcaga
      721 acgaaaacgc tggttagcac cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa
      781 ctggtcgagc gatggatttc cgtctctggt gtagctgatg atccgaataa ctacctgttt
      841 tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct atcaactcgc
      901 gccctggaag ggatttttga agcaactcat cgattgattt acggcgctaa ggatgactct
      961 ggtcagagat acctggcctg gtctggacac agtgcccgtg tcggagccgc gcgagatatg
     1021 gcccgcgctg gagtttcaat accggagatc atgcaagctg gtggctggac caatgtaaat
     1081 attgtcatga actatatccg taacctggat agtgaaacag gggcaatggt gcgcctgctg
     1141 gaagatggcg attaagaatt tactagagtc acactggctc accttcgggt gggcctttct
     1201 gcgtttatat actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta
     1261 ttt
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