LOCUS       BBa_K313022             1311 bp    DNA     linear       11-May-2024
DEFINITION  terminator leakiness assay device
ACCESSION   BBa_K313022
VERSION     BBa_K313022.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    55..94
                     /label=B1007
     stem_loop       64..85
                     /label=modified thr
     polyA_site      58..63
                     /label=PolyA
     polyA_site      86..91
                     /label=PolyA
     terminator      55..94
                     /label=BBa_B1007
     misc_feature    103..142
                     /label=B1007
     stem_loop       112..133
                     /label=modified thr
     polyA_site      106..111
                     /label=PolyA
     polyA_site      134..139
                     /label=PolyA
     terminator      103..142
                     /label=BBa_B1007
     RBS             151..165
                     /label=RBS-1\Strong
     RBS             158..162
                     /label=RBS
     misc_feature    151..165
                     /label=BBa_B0030
     RBS             151..165
                     /label=BBa_B0030
     CDS             172..1208
                     /label=BBa_J61047
     stem_loop       1224..1243
                     /label=T7 TE
     misc_feature    1250..1250
                     /label=stop
     polyA_site      1244..1257
                     /label=polya
     misc_feature    1217..1257
                     /label=BBa_B0012
     terminator      1217..1257
                     /label=BBa_B0012
     stem_loop       1278..1300
                     /label=stem_loop
     misc_feature    1266..1311
                     /label=BBa_B0011
     terminator      1266..1311
                     /label=BBa_B0011
     terminator      1217..1311
                     /label=BBa_B0014
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagcgcaaa
       61 aaaccccgcc cctgacaggg cggggttttt tcgctactag agcgcaaaaa accccgcccc
      121 tgacagggcg gggttttttc gctactagag attaaagagg agaaatacta gatgtccaat
      181 ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt
      241 cgcaagaacc tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa
      301 atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg
      361 tttcccgcag aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg
      421 gcagtaaaaa ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtccggg
      481 ctgccacgac caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa
      541 aacgttgatg ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac
      601 caggttcgtt cactcatgga aaatagcgat cgctgccagg atatacgtaa tctggcattt
      661 ctggggattg cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa
      721 gatatctcac gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg
      781 gttagcaccg caggtgtaga gaaggcactt agcctggggg taactaaact ggtcgagcga
      841 tggatttccg tctctggtgt agctgatgat ccgaataact acctgttttg ccgggtcaga
      901 aaaaatggtg ttgccgcgcc atctgccacc agccagctat caactcgcgc cctggaaggg
      961 atttttgaag caactcatcg attgatttac ggcgctaagg atgactctgg tcagagatac
     1021 ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga
     1081 gtttcaatac cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac
     1141 tatatccgta acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat
     1201 taagaattta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
     1261 tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt t
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