BBa_K313022
type: ComponentDefinition
persistent identity: BBa K313022
display id: BBa_K313022
version: 1
was generated by: iGEM to SBOL conversion
description: terminator leakiness assay device
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:11:54Z
mutable provenance: BBa_I712074, BBa_B1007, BBa_B0030, BBa_J61047, BBa_B0014
owned by: james
owned by: myers
mutable description: This is a device that aims to measure the leakiness of terminator BBa_B1007.
discontinued: false
dominant: false
experience: experience/None
group u list: _433_
m user id: 0
owner id: 5929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: nothing special
star: false
creator: Ryosuke Kamei, Ryo Kariyazono
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_B0014
component: BBa_I712074
component: BBa_J61047
component: BBa_B0030
component: BBa_B1007
sequence annotation: BBa_I712074
sequence annotation: BBa_B1007
sequence annotation: BBa_B0030
sequence annotation: BBa_J61047
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K313022_sequence/1

BBa_B0014
type: Component
persistent identity: component2093041
display id: component2093041
version: 1
top level: BBa_K313022
definition: BBa_B0014
access: public

BBa_I712074
type: Component
persistent identity: component2093020
display id: component2093020
version: 1
top level: BBa_K313022
definition: BBa_I712074
access: public

BBa_J61047
type: Component
persistent identity: component2093034
display id: component2093034
version: 1
top level: BBa_K313022
definition: Cre
access: public

BBa_B0030
type: Component
persistent identity: component2093032
display id: component2093032
version: 1
top level: BBa_K313022
definition: BBa_B0030
access: public

BBa_B1007
type: Component
persistent identity: component2093030
display id: component2093030
version: 1
top level: BBa_K313022
definition: BBa_B1007
access: public

BBa_B1007
type: Component
persistent identity: component2093025
display id: component2093025
version: 1
top level: BBa_K313022
definition: BBa_B1007
access: public

BBa_I712074
type: SequenceAnnotation
persistent identity: annotation2093020
display id: annotation2093020
version: 1
top level: BBa_K313022
location: https://synbiohub.org/public/igem/BBa_K313022/annotation2093020/range2093020/1
component: BBa_I712074

https://synbiohub.org/public/igem/BBa_K313022/annotation2093020/range2093020/1
type: Range
persistent identity: range2093020
display id: range2093020
version: 1
top level: BBa_K313022
direction: direction/reverse
start: 1
end: 46
orientation: inline

BBa_B1007
type: SequenceAnnotation
persistent identity: annotation2093025
display id: annotation2093025
version: 1
top level: BBa_K313022
location: https://synbiohub.org/public/igem/BBa_K313022/annotation2093025/range2093025/1
component: BBa_B1007

https://synbiohub.org/public/igem/BBa_K313022/annotation2093025/range2093025/1
type: Range
persistent identity: range2093025
display id: range2093025
version: 1
top level: BBa_K313022
direction: direction/reverse
start: 55
end: 94
orientation: inline

BBa_B1007
type: SequenceAnnotation
persistent identity: annotation2093030
display id: annotation2093030
version: 1
top level: BBa_K313022
location: https://synbiohub.org/public/igem/BBa_K313022/annotation2093030/range2093030/1
component: BBa_B1007

https://synbiohub.org/public/igem/BBa_K313022/annotation2093030/range2093030/1
type: Range
persistent identity: range2093030
display id: range2093030
version: 1
top level: BBa_K313022
direction: direction/reverse
start: 103
end: 142
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2093032
display id: annotation2093032
version: 1
top level: BBa_K313022
location: https://synbiohub.org/public/igem/BBa_K313022/annotation2093032/range2093032/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K313022/annotation2093032/range2093032/1
type: Range
persistent identity: range2093032
display id: range2093032
version: 1
top level: BBa_K313022
direction: direction/forward
start: 151
end: 165
orientation: inline

BBa_J61047
type: SequenceAnnotation
persistent identity: annotation2093034
display id: annotation2093034
version: 1
top level: BBa_K313022
location: https://synbiohub.org/public/igem/BBa_K313022/annotation2093034/range2093034/1
component: BBa_J61047

https://synbiohub.org/public/igem/BBa_K313022/annotation2093034/range2093034/1
type: Range
persistent identity: range2093034
display id: range2093034
version: 1
top level: BBa_K313022
direction: direction/reverse
start: 172
end: 1208
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2093041
display id: annotation2093041
version: 1
top level: BBa_K313022
location: https://synbiohub.org/public/igem/BBa_K313022/annotation2093041/range2093041/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K313022/annotation2093041/range2093041/1
type: Range
persistent identity: range2093041
display id: range2093041
version: 1
top level: BBa_K313022
direction: direction/reverse
start: 1217
end: 1311
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_I712074
type: ComponentDefinition
persistent identity: BBa I712074
display id: BBa_I712074
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter (strong promoter from T7 bacteriophage)
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:46Z
mutable provenance: T7 bacteriophage
owned by: james
owned by: myers
mutable description: T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1856
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Rok Gaber
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I712074_sequence/1

Cre
type: ComponentDefinition
persistent identity: BBa J61047
display id: BBa_J61047
version: 1
was generated by: iGEM to SBOL conversion
description: Cre DNA recombinase
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: bob
owned by: james
owned by: myers
mutable description: bob
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: bob
star: true
creator: John Anderson
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J61047_sequence/1

BBa_B1007
type: ComponentDefinition
persistent identity: BBa B1007
display id: BBa_B1007
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~=80)
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~=80% # 8bp stem, 6nt loop # Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 6 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1007
sequence annotation: PolyA
sequence annotation: modified thr
sequence: https://synbiohub.org/public/igem/BBa_B1007_sequence/1

B1007
type: SequenceAnnotation
persistent identity: annotation1900955
display id: annotation1900955
version: 1
top level: BBa_B1007
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900955/range1900955/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B1007/annotation1900955/range1900955/1
type: Range
persistent identity: range1900955
display id: range1900955
version: 1
top level: BBa_B1007
direction: direction/forward
start: 1
end: 40
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1900957
display id: annotation1900957
version: 1
top level: BBa_B1007
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900957/range1900957/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1007/annotation1900957/range1900957/1
type: Range
persistent identity: range1900957
display id: range1900957
version: 1
top level: BBa_B1007
direction: direction/reverse
start: 4
end: 9
orientation: inline

modified thr
type: SequenceAnnotation
persistent identity: annotation1900956
display id: annotation1900956
version: 1
top level: BBa_B1007
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900956/range1900956/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1007/annotation1900956/range1900956/1
type: Range
persistent identity: range1900956
display id: range1900956
version: 1
top level: BBa_B1007
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1900958
display id: annotation1900958
version: 1
top level: BBa_B1007
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900958/range1900958/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1007/annotation1900958/range1900958/1
type: Range
persistent identity: range1900958
display id: range1900958
version: 1
top level: BBa_B1007
direction: direction/forward
start: 32
end: 37
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K313022_sequence/1
type: Sequence
persistent identity: BBa K313022 sequence
display id: BBa_K313022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaatacaagctacttgttctttttgcatactagagcgcaaaaaaccccgcccctgacagggcggggttttttcgctactagagcgcaaaaaaccccgcccctgacagggcggggttttttcgctactagagattaaagaggagaaatactagatgtccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgattaagaatttactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I712074_sequence/1
type: Sequence
persistent identity: BBa I712074 sequence
display id: BBa_I712074_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaatacaagctacttgttctttttgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61047_sequence/1
type: Sequence
persistent identity: BBa J61047 sequence
display id: BBa_J61047_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgattaagaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1007_sequence/1
type: Sequence
persistent identity: BBa B1007 sequence
display id: BBa_B1007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgcaaaaaaccccgcccctgacagggcggggttttttcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z