LOCUS       BBa_K313027             1403 bp    DNA     linear       13-May-2024
DEFINITION  terminator leakiness assay device
ACCESSION   BBa_K313027
VERSION     BBa_K313027.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    55..93
                     /label=B1006
     stem_loop       64..85
                     /label=modified thr terminator
     polyA_site      86..93
                     /label=PolyA
     polyA_site      55..63
                     /label=PolyA
     terminator      55..93
                     /label=BBa_B1006
     misc_feature    102..140
                     /label=B1006
     stem_loop       111..132
                     /label=modified thr terminator
     polyA_site      133..140
                     /label=PolyA
     polyA_site      102..110
                     /label=PolyA
     terminator      102..140
                     /label=BBa_B1006
     misc_feature    149..187
                     /label=B1006
     stem_loop       158..179
                     /label=modified thr terminator
     polyA_site      180..187
                     /label=PolyA
     polyA_site      149..157
                     /label=PolyA
     terminator      149..187
                     /label=BBa_B1006
     misc_feature    196..234
                     /label=B1006
     stem_loop       205..226
                     /label=modified thr terminator
     polyA_site      227..234
                     /label=PolyA
     polyA_site      196..204
                     /label=PolyA
     terminator      196..234
                     /label=BBa_B1006
     RBS             243..257
                     /label=RBS-1\Strong
     RBS             250..254
                     /label=RBS
     misc_feature    243..257
                     /label=BBa_B0030
     RBS             243..257
                     /label=BBa_B0030
     CDS             264..1300
                     /label=BBa_J61047
     stem_loop       1316..1335
                     /label=T7 TE
     misc_feature    1342..1342
                     /label=stop
     polyA_site      1336..1349
                     /label=polya
     misc_feature    1309..1349
                     /label=BBa_B0012
     terminator      1309..1349
                     /label=BBa_B0012
     stem_loop       1370..1392
                     /label=stem_loop
     misc_feature    1358..1403
                     /label=BBa_B0011
     terminator      1358..1403
                     /label=BBa_B0011
     terminator      1309..1403
                     /label=BBa_B0014
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaaaaa
       61 aaaccccgcc cctgacaggg cggggttttt ttttactaga gaaaaaaaaa ccccgcccct
      121 gacagggcgg ggtttttttt tactagagaa aaaaaaaccc cgcccctgac agggcggggt
      181 ttttttttac tagagaaaaa aaaaccccgc ccctgacagg gcggggtttt tttttactag
      241 agattaaaga ggagaaatac tagatgtcca atttactgac cgtacaccaa aatttgcctg
      301 cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac atgttcaggg
      361 atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc cggtcgtggg
      421 cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg
      481 attatcttct atatcttcag gcgcgcggtc tggcagtaaa aactatccag caacatttgg
      541 gccagctaaa catgcttcat cgtcggtccg ggctgccacg accaagtgac agcaatgctg
      601 tttcactggt tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac
      661 aggctctagc gttcgaacgc actgatttcg accaggttcg ttcactcatg gaaaatagcg
      721 atcgctgcca ggatatacgt aatctggcat ttctggggat tgcttataac accctgttac
      781 gtatagccga aattgccagg atcagggtta aagatatctc acgtactgac ggtgggagaa
      841 tgttaatcca tattggcaga acgaaaacgc tggttagcac cgcaggtgta gagaaggcac
      901 ttagcctggg ggtaactaaa ctggtcgagc gatggatttc cgtctctggt gtagctgatg
      961 atccgaataa ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca
     1021 ccagccagct atcaactcgc gccctggaag ggatttttga agcaactcat cgattgattt
     1081 acggcgctaa ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg
     1141 tcggagccgc gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg
     1201 gtggctggac caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag
     1261 gggcaatggt gcgcctgctg gaagatggcg attaagaatt tactagagtc acactggctc
     1321 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta
     1381 ttaatccggc ttttttatta ttt
//