LOCUS       BBa_K313030              778 bp    DNA     linear       12-May-2024
DEFINITION  signal transmission assay device
ACCESSION   BBa_K313030
VERSION     BBa_K313030.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    55..73
                     /label=BBa_K313010
     RBS             82..96
                     /label=RBS-1\Strong
     RBS             89..93
                     /label=RBS
     misc_feature    82..96
                     /label=BBa_B0030
     RBS             82..96
                     /label=BBa_B0030
     misc_feature    172..177
                     /label=Former SpeI site
     misc_feature    174..174
                     /label=Mutation to delete the SpeI site
     CDS             103..675
                     /label=Hin sequence
     primer_bind     103..105
                     /label=Fwd primer J31001
     primer_bind     653..672
                     /label=Rev primer J31001
     CDS             103..675
                     /label=BBa_J31000
     stem_loop       691..710
                     /label=T7 TE
     misc_feature    717..717
                     /label=stop
     polyA_site      711..724
                     /label=polya
     misc_feature    684..724
                     /label=BBa_B0012
     terminator      684..724
                     /label=BBa_B0012
     stem_loop       745..767
                     /label=stem_loop
     misc_feature    733..778
                     /label=BBa_B0011
     terminator      733..778
                     /label=BBa_B0011
     terminator      684..778
                     /label=BBa_B0014
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagacatga
       61 ggattaccca tgttactaga gattaaagag gagaaatact agatggctac tattgggtat
      121 attcgggtgt caacaattga ccaaaatatc gatttacagc gtaatgcgct taccagtgca
      181 aattgtgacc gcatttttga agaccgtatc agtggcaaga ttgcaaaccg ccccggcctg
      241 aaacgggcgt taaagtatgt aaataaaggc gatactcttg tcgtctggaa attagacaga
      301 ctgggccgta gcgtgaaaaa tctggtggcg ttaatatcag aattacatga acgtggagct
      361 cacttccatt ctttaaccga tagtattgat accagtagcg cgatggggcg attctttttt
      421 catgtaatgt cagcactggc cgagatggag cgagaattaa tcgtcgagcg aacccttgcc
      481 ggactggctg ccgccagagc gcaaggacga ctgggagggc gccctcgggc gatcaacaaa
      541 catgaacagg aacagattag tcggctatta gagaaaggcc atcctcggca gcaattagct
      601 attatttttg gtattggcgt atccacctta tacagatact ttccggcaag cagtataaaa
      661 aaacgaatga attaatacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      721 tatatactag agagagaata taaaaagcca gattattaat ccggcttttt tattattt
//