LOCUS       BBa_K313035             1086 bp    DNA     linear       12-May-2024
DEFINITION  Hin invertase assay device
ACCESSION   BBa_K313035
VERSION     BBa_K313035.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     misc_feature    55..80
                     /label=BBa_J44000
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     stem_loop       150..172
                     /label=stem_loop
     misc_feature    138..183
                     /label=BBa_B0011
     terminator      138..183
                     /label=BBa_B0011
     terminator      89..183
                     /label=BBa_B0014
     misc_feature    192..217
                     /label=BBa_J44000
     misc_feature    230..233
                     /label=conserved
     RBS             226..237
                     /label=BBa_B0034
     CDS             244..918
                     /label=mrfp1
     misc_feature    925..949
                     /label=Help:Barcodes
     CDS             244..924
                     /label=BBa_E1010
     stem_loop       969..1012
                     /label=stem_loop
     misc_feature    958..1037
                     /label=BBa_B0010
     terminator      958..1037
                     /label=BBa_B0010
     stem_loop       1053..1072
                     /label=T7 TE
     misc_feature    1079..1079
                     /label=stop
     polyA_site      1073..1086
                     /label=polya
     misc_feature    1046..1086
                     /label=BBa_B0012
     terminator      1046..1086
                     /label=BBa_B0012
     misc_feature    226..1086
                     /label=BBa_I13507
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagttatca
       61 aaaaccatgg tttttgataa tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta
      181 ttttactaga gttatcaaaa accatggttt ttgataatac tagagaaaga ggagaaatac
      241 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
      301 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
      361 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
      421 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
      481 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
      541 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
      601 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
      661 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
      721 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
      781 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
      841 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
      901 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca
      961 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1021 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1081 tttata
//