BBa_K313036 1 BBa_K313036 MS2 phage cDNA (loading sequence and replicase-deficient) 2010-10-25T11:00:00Z 2015-05-08T01:11:54Z We bought MS2 virus as RNA and made cDNA by reverse transcription. Loading sequence deficient MS2 phage cDNA. Virus transformed from this part carry out mRNA with specific loading sequence(19bp) and infect another E.coli. In the infected E.coli, translation of mRNA occur. false false _433_ 0 5939 9 It's complicated true We removed XbaI site, loading sequence and replicase region. false Tomoka Ao, Ryosuke Kamei, Ryo Kariyazono, Masahiko Shimizu, Kosuke Uekusa, Mami Nakatake BBa_K313036_sequence 1 gggtgggacccctttcggggtcctgctcaacttcctgtcgagctaatgccatttttaatgtctttagcgagacgctaccatggctatcgctgtaggtagccggaattccattcctaggaggtttgacctgtgcgagcttttagtacccttgatagggagaacgagaccttcgtcccctccgttcgcgtttacgcggacggtgagactgaagataactcattctctttaaaatatcgttcgaactggactcccggtcgttttaactcgactggggccaaaacgaaacagtggcactacccctctccgtattcacggggggcgttaagtgtcacatcgatagatcaaggtgcctacaagcgaagtgggtcatcgtggggtcgcccgtacgaggagaaagccggtttcggcttctccctcgacgcacgctcctgctacagcctcttccctgtaagccaaaacttgacttacatcgaagtgccgcagaacgttgcgaaccgggcgtcgaccgaagtcctgcaaaaggtcacccagggtaattttaaccttggtgttgctttagcagaggccaggtcgacagcctcacaactcgcgacgcaaaccattgcgctcgtgaaggcgtacactgccgctcgtcgcggtaattggcgccaggcgctccgctaccttgccctaaacgaagatcgaaagtttcgatcaaaacacgtggccggcaggtggttggagttgcagttcggttggttaccactaatgagtgatatccagggtgcatatgagatgcttacgaaggttcaccttcaagagtttcttcctatgagagccgtacgtcaggtcggtactaacatcaagttagatggccgtctgtcgtatccagctgcaaacttccagacaacgtgcaacatatcgcgacgtatcgtgatatggttttacataaacgatgcacgtttggcatggttgtcgtctctaggtatcttgaacccactaggtatagtgtgggaaaaggtgcctttctcattcgttgtcgactggctcctacctgtaggtaacatgctcgagggccttacggcccccgtgggatgctcctacatgtcaggaacagttactgacgtaataacgggtgagtccatcataagcgttgacgctccctacgggtggactgtggagagacagggcactgctaaggcccaaatctcagccatgcatcgaggggtacaatccgtatggccaacaactggcgcgtacgtaaagtctcctttctcgatggtccataccttagatgcgttagcattaatcaggcaacggctctctcgatagagccctcaaccggagtttgaagcatggcttctaactttactcagttcgttctcgtcgacaatggcggaactggcgacgtgactgtcgccccaagcaacttcgctaacggggtcgctgaatggatcagctctaactcgcgttcacaggcttacaaagtaacctgtagcgttcgtcagagctctgcgcagaatcgcaaatacaccatcaaagtcgaggtgcctaaagtggcaacccagactgttggtggtgtagagcttcctgtagccgcatggcgttcgtacttaaatatggaactaaccattccaattttcgctacgaattccgactgcgagcttattgttaaggcaatgcaaggtctcctaaaagatggaaacccgattccctcagcaatcgcagcaaactccggcatctactaatagacgccggccattcaaacatgaagattatccgtgccgaagacaacaaagaagttcaactctttatgtattgatcttcctcgcgatctttctctcgaaatttaccaatcaattgcttctgtcgctactggaagcggtgatccgcacagtgacgactttacagcaattgcttacttaagggacgaattgctcacaaagcatccgaccttaggttctggtaatgacgaggcgacccgtcgtaccttagctatcgctaagctacgggaggcgaatgatcggtgcggtcagataaatagagaaggtttcttacatgacaaatccttgtcatgggatccggatgttttacaaaccagcatccgtagccttattggcaacctcctctctggctaccgatcgtcgttgtttgggcaatgcacgttctccaacggtgcctctatggggcacaagttgcaggatgcagcgccttacaagaagttcgctgaacaagcaaccgttaccccccgcgctctgagagcggctctattggtccgagaccaatgtgcgccgtggatcagacacgcggtccgctataacgagtcatatgaatttaggctcgttgtagggaacggagtgtttacagttccgaagaataataaaatagattgggctgcctgtaaggagcctgatatgaatatgtacctccagaaaggggtcggtgcctttatcagacgccggctcaaatccgttggtatagacctgaatgatcaatcgatcaaccagcgtctggctcagcagggcagcgtagatggttcgcttgcgacgatagacttatcgtctgcatccgattccatctccgatcgcctggtgtggagttttctcccaccagagctatattcatatctcgatcgtatccgctcacactacggaatcgtagatggcgagacgatacgatgggaactattttccacaatgggaaatgggttcacatttgagctagagtccatgatattctgggcaatagtcaaagcgacccaaatccattttggtaacgccggaaccataggcatctacggggacgatattatatgtcccagtgagattgcaccccgtgtgctagaggcacttgcctactacggttttaaaccgaatcttcgtaaaacgttcgtgtccgggctctttcgcgagagctgcggcgcgcacttttaccgtggtgtcgatgtcaaaccgttttacatcaagaaacctgttgacaatctcttcgccctgatgctgatattaaatcggctacggggttggggagttgtcggaggtatgtcagatccacgcctctacaaggtgtgggtacggctctcctcccaggtgccttcgatgttcttcggtgggacggacctcgctgccgactactacgtagtcagcccgcctacggcagtctcggtatacaccaagactccgtacgggcggctgctcgcggatacccgtacctcgggtttccgtcttgctcgtatcgctcgagaacgcaagttcttcagcgaaaagcacgacagtggtcgctacatagcgtggttccatactggaggtgaaatcaccgacagcatgaagtccgccggcgtgcgcgttatacgcacttcggagtggctaacgccggttcccacattccctcaggagtgtgggccagcgagctctcctcggtagctgaccgagggacccccgtaaacggggtgggtgtgctcgaaagagcacgggtccgcgaaagcggtggctccaccgaaaggtgggcgggcttcggcccagggacctccccctaaagagaggacccgggattctcccgatttggtaactagctgcttggctagttaccaccca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z