LOCUS       BBa_K314203             1807 bp    DNA     linear       23-Dec-2024
DEFINITION  HSL inducible toxin antitoxin, Tse2 + Tsi2
ACCESSION   BBa_K314203
VERSION     BBa_K314203.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     misc_feature    1088..1564
                     /label=tse2
     misc_feature    1574..1807
                     /label=tsi2
     CDS             1088..1807
                     /label=BBa_K314202
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg tcctacgact acgagaaaac cagcctcacc ctctaccggg cggtattcaa
     1141 ggccaactac gacggcgacg tcggtcgcta cctgcatccc gacaaggaac tcgccgaggc
     1201 tgcggaagtc gccccgctgc tgcatccgac cttcgacagc cccaacaccc ctggcgtccc
     1261 cgcccgcgcg ccggacatcg tcgccggccg cgacggcctc tacgccccgg acaccggcgg
     1321 cacctcggtg ttcgaccgcg ccggcgtgct gcgccgcgcc gacggcgact tcgtgatacc
     1381 cgacggcacc gacatcccgc cggaccttaa ggtgaagcag gacagctaca acaagcgcct
     1441 gcaagccacc cactacacca tcatgccggc caagccgatg taccgggagg tcctcatggg
     1501 ccaactggac aacttcgtgc gcaacgccat ccgccgccaa tgggaaaaag cccgcgggct
     1561 ctaggagtga accatgaacc tgaaacccca gaccctgatg gtggcgatcc agtgcgtcgc
     1621 cgcgcgcacc cgcgaactcg acgcgcagtt gcagaacgac gacccgcaga acgccgctga
     1681 actcgaacag ttgctggtcg gctacgacct cgccgccgac gacctgaaga acgcctacga
     1741 gcaagccctg ggccaataca gcggcctgcc gccctatgac cggctgatcg aagagccggc
     1801 atcctga
//