Original source was from ''P. aeruginosa'' PA01 genome library--fosmid O24--from Mike Jacobs at University of Washington Department of Immunology.
A fosmid (F' BAC) which encodes the HSI-I Type VI Secretion locus of ''Pseudomonas aeruginosa''. The locus has been engineered using homologous recombination to replace native promoters with robust T7 promoters. The two promoters are contained in the same intergenic region, with one on the positive strand and one on the negative. We are using this plasmid to express a Type VI Secretion System in the BL21(DE3) strain of ''E. coli'' which has an IPTG inducible T7 RNA polymerase. The purpose of the system is to create a probiotic system, creating a secretion system for a toxin/antitoxin system (BBa_K314200-BBa_K3142003). The system allows for protein delivery directly from the donor cell into the periplasm or cytosol of the recepient organism. While we are using this system to deliver a toxin, other protein effectors might be used for a range of applications.
The size of the gene locus was too large to effectively BioBrick; there are several NotI and other restriction sites in crucial parts of necessary genes.
Type six secretion system from Pseudomonas aeruginosain a pCC2Fos CopyControl Fosmid.
Conversion of the iGEM parts registry to SBOL2.1