BBa_K314204
type: ComponentDefinition
persistent identity: BBa K314204
display id: BBa_K314204
version: 1
was generated by: iGEM to SBOL conversion
description: p4T6S::T7
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:11:55Z
mutable provenance: Original source was from ''P. aeruginosa'' PA01 genome library--fosmid O24--from Mike Jacobs at University of Washington Department of Immunology.
owned by: james
owned by: myers
mutable description: A fosmid (F' BAC) which encodes the HSI-I Type VI Secretion locus of ''Pseudomonas aeruginosa''. The locus has been engineered using homologous recombination to replace native promoters with robust T7 promoters. The two promoters are contained in the same intergenic region, with one on the positive strand and one on the negative. We are using this plasmid to express a Type VI Secretion System in the BL21(DE3) strain of ''E. coli'' which has an IPTG inducible T7 RNA polymerase. The purpose of the system is to create a probiotic system, creating a secretion system for a toxin/antitoxin system (BBa_K314200-BBa_K3142003). The system allows for protein delivery directly from the donor cell into the periplasm or cytosol of the recepient organism. While we are using this system to deliver a toxin, other protein effectors might be used for a range of applications.
discontinued: true
dominant: false
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6398
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: The size of the gene locus was too large to effectively BioBrick; there are several NotI and other restriction sites in crucial parts of necessary genes.
star: false
is replaced by: T6SS
creator: Laura Austin
type: DnaRegion
role: partType/Plasmid
role: SO:0000155
sequence annotation: ppkA
sequence annotation: pppA
sequence annotation: icmF1
sequence annotation: fha1
sequence annotation: RBS
sequence annotation: T7 promoter
sequence annotation: hcp1
sequence annotation: clpV1
sequence annotation: VgrG1
sequence: https://synbiohub.org/public/igem/BBa_K314204_sequence/1

ppkA
type: SequenceAnnotation
persistent identity: annotation2095336
display id: annotation2095336
version: 1
top level: BBa_K314204
location: https://synbiohub.org/public/igem/BBa_K314204/annotation2095336/range2095336/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K314204/annotation2095336/range2095336/1
type: Range
persistent identity: range2095336
display id: range2095336
version: 1
top level: BBa_K314204
direction: direction/reverse
start: 13050
end: 16148
orientation: inline

pppA
type: SequenceAnnotation
persistent identity: annotation2095337
display id: annotation2095337
version: 1
top level: BBa_K314204
location: https://synbiohub.org/public/igem/BBa_K314204/annotation2095337/range2095337/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K314204/annotation2095337/range2095337/1
type: Range
persistent identity: range2095337
display id: range2095337
version: 1
top level: BBa_K314204
direction: direction/reverse
start: 16156
end: 16884
orientation: inline

icmF1
type: SequenceAnnotation
persistent identity: annotation2095338
display id: annotation2095338
version: 1
top level: BBa_K314204
location: https://synbiohub.org/public/igem/BBa_K314204/annotation2095338/range2095338/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K314204/annotation2095338/range2095338/1
type: Range
persistent identity: range2095338
display id: range2095338
version: 1
top level: BBa_K314204
direction: direction/reverse
start: 17571
end: 20876
orientation: inline

fha1
type: SequenceAnnotation
persistent identity: annotation2095339
display id: annotation2095339
version: 1
top level: BBa_K314204
location: https://synbiohub.org/public/igem/BBa_K314204/annotation2095339/range2095339/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K314204/annotation2095339/range2095339/1
type: Range
persistent identity: range2095339
display id: range2095339
version: 1
top level: BBa_K314204
direction: direction/reverse
start: 24313
end: 25806
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2095340
display id: annotation2095340
version: 1
top level: BBa_K314204
location: https://synbiohub.org/public/igem/BBa_K314204/annotation2095340/range2095340/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K314204/annotation2095340/range2095340/1
type: Range
persistent identity: range2095340
display id: range2095340
version: 1
top level: BBa_K314204
direction: direction/reverse
start: 25812
end: 25817
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2095341
display id: annotation2095341
version: 1
top level: BBa_K314204
location: https://synbiohub.org/public/igem/BBa_K314204/annotation2095341/range2095341/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K314204/annotation2095341/range2095341/1
type: Range
persistent identity: range2095341
display id: range2095341
version: 1
top level: BBa_K314204
direction: direction/reverse
start: 25825
end: 25844
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2095342
display id: annotation2095342
version: 1
top level: BBa_K314204
location: https://synbiohub.org/public/igem/BBa_K314204/annotation2095342/range2095342/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K314204/annotation2095342/range2095342/1
type: Range
persistent identity: range2095342
display id: range2095342
version: 1
top level: BBa_K314204
direction: direction/forward
start: 25876
end: 25894
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2095343
display id: annotation2095343
version: 1
top level: BBa_K314204
location: https://synbiohub.org/public/igem/BBa_K314204/annotation2095343/range2095343/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K314204/annotation2095343/range2095343/1
type: Range
persistent identity: range2095343
display id: range2095343
version: 1
top level: BBa_K314204
direction: direction/forward
start: 25900
end: 25905
orientation: inline

hcp1
type: SequenceAnnotation
persistent identity: annotation2095346
display id: annotation2095346
version: 1
top level: BBa_K314204
location: https://synbiohub.org/public/igem/BBa_K314204/annotation2095346/range2095346/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K314204/annotation2095346/range2095346/1
type: Range
persistent identity: range2095346
display id: range2095346
version: 1
top level: BBa_K314204
direction: direction/forward
start: 29141
end: 29629
orientation: inline

clpV1
type: SequenceAnnotation
persistent identity: annotation2095347
display id: annotation2095347
version: 1
top level: BBa_K314204
location: https://synbiohub.org/public/igem/BBa_K314204/annotation2095347/range2095347/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K314204/annotation2095347/range2095347/1
type: Range
persistent identity: range2095347
display id: range2095347
version: 1
top level: BBa_K314204
direction: direction/forward
start: 34011
end: 36719
orientation: inline

VgrG1
type: SequenceAnnotation
persistent identity: annotation2095348
display id: annotation2095348
version: 1
top level: BBa_K314204
location: https://synbiohub.org/public/igem/BBa_K314204/annotation2095348/range2095348/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K314204/annotation2095348/range2095348/1
type: Range
persistent identity: range2095348
display id: range2095348
version: 1
top level: BBa_K314204
direction: direction/forward
start: 36766
end: 38697
orientation: inline

T6SS
type: ComponentDefinition
persistent identity: BBa K314300
display id: BBa_K314300
version: 1
was generated by: iGEM to SBOL conversion
description: T6SS fosmid
created: 2010-10-21T11:00:00Z
modified: 2015-05-08T01:11:55Z
mutable provenance: Pseudomonas aeruginosain
owned by: james
owned by: myers
mutable description: Type six secretion system from Pseudomonas aeruginosain a pCC2Fos CopyControl Fosmid.
discontinued: false
dominant: true
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6501
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: space holder
star: false
creator: Laura Austin
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: ppkA
sequence annotation: pppA
sequence annotation: icmF1
sequence annotation: fha1
sequence annotation: RBS
sequence annotation: T7 promoter
sequence annotation: hcp1
sequence annotation: clpV1
sequence annotation: VgrG1
sequence: https://synbiohub.org/public/igem/BBa_K314300_sequence/1

ppkA
type: SequenceAnnotation
persistent identity: annotation2109311
display id: annotation2109311
version: 1
top level: T6SS
location: https://synbiohub.org/public/igem/BBa_K314300/annotation2109311/range2109311/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K314300/annotation2109311/range2109311/1
type: Range
persistent identity: range2109311
display id: range2109311
version: 1
top level: T6SS
direction: direction/reverse
start: 13048
end: 16146
orientation: inline

pppA
type: SequenceAnnotation
persistent identity: annotation2109312
display id: annotation2109312
version: 1
top level: T6SS
location: https://synbiohub.org/public/igem/BBa_K314300/annotation2109312/range2109312/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K314300/annotation2109312/range2109312/1
type: Range
persistent identity: range2109312
display id: range2109312
version: 1
top level: T6SS
direction: direction/reverse
start: 16153
end: 16881
orientation: inline

icmF1
type: SequenceAnnotation
persistent identity: annotation2109313
display id: annotation2109313
version: 1
top level: T6SS
location: https://synbiohub.org/public/igem/BBa_K314300/annotation2109313/range2109313/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K314300/annotation2109313/range2109313/1
type: Range
persistent identity: range2109313
display id: range2109313
version: 1
top level: T6SS
direction: direction/reverse
start: 17568
end: 20872
orientation: inline

fha1
type: SequenceAnnotation
persistent identity: annotation2109314
display id: annotation2109314
version: 1
top level: T6SS
location: https://synbiohub.org/public/igem/BBa_K314300/annotation2109314/range2109314/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K314300/annotation2109314/range2109314/1
type: Range
persistent identity: range2109314
display id: range2109314
version: 1
top level: T6SS
direction: direction/reverse
start: 24312
end: 25806
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2109320
display id: annotation2109320
version: 1
top level: T6SS
location: https://synbiohub.org/public/igem/BBa_K314300/annotation2109320/range2109320/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K314300/annotation2109320/range2109320/1
type: Range
persistent identity: range2109320
display id: range2109320
version: 1
top level: T6SS
direction: direction/reverse
start: 25812
end: 25817
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2109335
display id: annotation2109335
version: 1
top level: T6SS
location: https://synbiohub.org/public/igem/BBa_K314300/annotation2109335/range2109335/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K314300/annotation2109335/range2109335/1
type: Range
persistent identity: range2109335
display id: range2109335
version: 1
top level: T6SS
direction: direction/reverse
start: 25825
end: 25844
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2109383
display id: annotation2109383
version: 1
top level: T6SS
location: https://synbiohub.org/public/igem/BBa_K314300/annotation2109383/range2109383/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K314300/annotation2109383/range2109383/1
type: Range
persistent identity: range2109383
display id: range2109383
version: 1
top level: T6SS
direction: direction/forward
start: 25876
end: 25894
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2109387
display id: annotation2109387
version: 1
top level: T6SS
location: https://synbiohub.org/public/igem/BBa_K314300/annotation2109387/range2109387/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K314300/annotation2109387/range2109387/1
type: Range
persistent identity: range2109387
display id: range2109387
version: 1
top level: T6SS
direction: direction/forward
start: 25900
end: 25905
orientation: inline

hcp1
type: SequenceAnnotation
persistent identity: annotation2109315
display id: annotation2109315
version: 1
top level: T6SS
location: https://synbiohub.org/public/igem/BBa_K314300/annotation2109315/range2109315/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K314300/annotation2109315/range2109315/1
type: Range
persistent identity: range2109315
display id: range2109315
version: 1
top level: T6SS
direction: direction/forward
start: 29150
end: 29628
orientation: inline

clpV1
type: SequenceAnnotation
persistent identity: annotation2109316
display id: annotation2109316
version: 1
top level: T6SS
location: https://synbiohub.org/public/igem/BBa_K314300/annotation2109316/range2109316/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K314300/annotation2109316/range2109316/1
type: Range
persistent identity: range2109316
display id: range2109316
version: 1
top level: T6SS
direction: direction/forward
start: 34011
end: 36719
orientation: inline

VgrG1
type: SequenceAnnotation
persistent identity: annotation2109317
display id: annotation2109317
version: 1
top level: T6SS
location: https://synbiohub.org/public/igem/BBa_K314300/annotation2109317/range2109317/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K314300/annotation2109317/range2109317/1
type: Range
persistent identity: range2109317
display id: range2109317
version: 1
top level: T6SS
direction: direction/forward
start: 36764
end: 38693
orientation: inline

https://synbiohub.org/public/igem/BBa_K314204_sequence/1
type: Sequence
persistent identity: BBa K314204 sequence
display id: BBa_K314204_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcggccgcaaggggttcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcagctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcactatagggcgaattcgagctcggtacccggggatcccaccgaacgggcgaccacctcgccacgctcgtcgctcagtagcgccagcgccctgcgcaattgcgggcgcacctccaggcgatagcgccggcccgcctcgaagccgtcatagaccacctgctggtagcagttgatctgcaccggctcgccgaagccgccccccccgccgccggaaccgccatagagttcgtactggaagcgcacctccagcacatggcgtcccggagtgacctggaagtagcgcccgtcgctcaaccgctcgccgtccaggcgctcggccagcagggtgttggccggcgcgctgcgcagctcgacccaggccatctgcgggtccggctgcggcagcggtccggcgcagccggcgaggagcagcagcgcgagcgggacgagacgggtgagtggacgcatggggcgggctccggagggtgttcagctcctagcatagcgccctcgccttgcgccgttcagaccttcgcgcagcgcccctcgcggccgcgcgccagttccctgttgtcctggtcgtagagcttcgcccagggacggaagccgatgtgcccggcctccaggcggtagcgctggccggcgctgaagtccttgaacttgaggttgagctggcaatcgcgccagagcgggctgtccacggggccgatgttggtcgggtcgacggtgaactggtagcgcaccgtgagttcgcgggtgccgggatcgacctcgaagtagcgcgtgcggtcccagctcttgtcgtcgacttccacggcggcgagatcggcgtcgggctgggtatcgaggtcgacccaggcgcggttcgggtcgggggtgggcatcagcagtgagcagccgctcaggacgagcaggccaccggcaagcagcagcgtacgcatagaagactccgaagctcctttaacccggccgccatccgtcgcgccttggaaaaacgccttgcgttcctccgcctcgccggatcgcggaccggtgctggtagggcggcgggcggtccgcgtgctgtgctatgttccgcccatgccacgccgccttcgccatccgcttgctgaccttcgactgccccgttgggttccctgcctggcgctgttggcgctgagcggttgctcgaccgtctcctactacgggcaactggccagcggccagttcgccctgctgcgcgcccgcgagccggccgccgcggtgatcgccgacccgcagcgcgacgcgcgcttgcgcgaacgcctggcgaaggcggaaaaggcccggcaattcgccagcgaacgcctcggcctgccggacaaccgcagctatcgcctgtacgccgatatcggccggccctacgtggtatggaacgtgttcgccaccccggagctgtcgctgcagccggtcacccattgcttccccatcgccggctgcgtggcctaccgcggctactacagcgaggccgccgcgcgcgccgaggcggcgcgccagcgggcgctcggcgacgacgtctacgtcggcggggtgcaggcctactcgaccctcggctggttcgacgatccgatcctcagcagcatgctgcgctgggacgacgaacggctggcgaccttgatcttccacgaactggcgcaccagaagttctacctgcgcgacgacaccgccttcaacgaatccttcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K314300_sequence/1
type: Sequence
persistent identity: BBa K314300 sequence
display id: BBa_K314300_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcggccgcaaggggttcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcagctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcactatagggcgaattcgagctcggtacccggggatcccaccgaacgggcgaccacctcgccacgctcgtcgctcagtagcgccagcgccctgcgcaattgcgggcgcacctccaggcgatagcgccggcccgcctcgaagccgtcatagaccacctgctggtagcagttgatctgcaccggctcgccgaagccgccccccccgccgccggaaccgccatagagttcgtactggaagcgcacctccagcacatggcgtcccggagtgacctggaagtagcgcccgtcgctcaaccgctcgccgtccaggcgctcggccagcagggtgttggccggcgcgctgcgcagctcgacccaggccatctgcgggtccggctgcggcagcggtccggcgcagccggcgaggagcagcagcgcgagcgggacgagacgggtgagtggacgcatggggcgggctccggagggtgttcagctcctagcatagcgccctcgccttgcgccgttcagaccttcgcgcagcgcccctcgcggccgcgcgccagttccctgttgtcctggtcgtagagcttcgcccagggacggaagccgatgtgcccggcctccaggcggtagcgctggccggcgctgaagtccttgaacttgaggttgagctggcaatcgcgccagagcgggctgtccacggggccgatgttggtcgggtcgacggtgaactggtagcgcaccgtgagttcgcgggtgccgggatcgacctcgaagtagcgcgtgcggtcccagctcttgtcgtcgacttccacggcggcgagatcggcgtcgggctgggtatcgaggtcgacccaggcgcggttcgggtcgggggtgggcatcagcagtgagcagccgctcaggacgagcaggccaccggcaagcagcagcgtacgcatagaagactccgaagctcctttaacccggccgccatccgtcgcgccttggaaaaacgccttgcgttcctccgcctcgccggatcgcggaccggtgctggtagggcggcgggcggtccgcgtgctgtgctatgttccgcccatgccacgccgccttcgccatccgcttgctgaccttcgactgccccgttgggttccctgcctggcgctgttggcgctgagcggttgctcgaccgtctcctactacgggcaactggccagcggccagttcgccctgctgcgcgcccgcgagccggccgccgcggtgatcgccgacccgcagcgcgacgcgcgcttgcgcgaacgcctggcgaaggcggaaaaggcccggcaattcgccagcgaacgcctcggcctgccggacaaccgcagctatcgcctgtacgccgatatcggccggccctacgtggtatggaacgtgttcgccaccccggagctgtcgctgcagccggtcacccattgcttccccatcgccggctgcgtggcctaccgcggctactacagcgaggccgccgcgcgcgccgaggcggcgcgccagcgggcgctcggcgacgacgtctacgtcggcggggtgcaggcctactcgaccctcggctggttcgacgatccgatcctcagcagcatgctgcgctgggacgacgaacggctggcgaccttgatcttccacgaactggcgcaccagaagttctacctgcgcgacgacaccgccttcaacgaatccttcg…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z