LOCUS       BBa_K315003             2149 bp    DNA     linear       29-May-2024
DEFINITION  pLac+RBS+TetA+RBS+RFP
ACCESSION   BBa_K315003
VERSION     BBa_K315003.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1417
                     /label=tetA(C) forward
     primer_bind     227..250
                     /label=Fwd primer J31007
     primer_bind     1398..1417
                     /label=Rev primer J31007
     misc_feature    1415..1417
                     /label=stop
     CDS             227..1417
                     /label=BBa_J31007
     misc_feature    209..1417
                     /label=BBa_S03710
     misc_feature    1430..1433
                     /label=conserved
     RBS             1426..1437
                     /label=BBa_B0034
     CDS             1444..2118
                     /label=mrfp1
     misc_feature    2125..2149
                     /label=Help:Barcodes
     CDS             1444..2124
                     /label=BBa_E1010
     misc_feature    1426..2149
                     /label=BBa_I13502
     misc_feature    209..2149
                     /label=BBa_S03720
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aatctaacaa
      241 tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat
      301 gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta
      361 tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc
      421 actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat
      481 cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt
      541 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
      601 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
      661 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
      721 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
      781 taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
      841 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
      901 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
      961 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
     1021 cgtcactggc cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
     1081 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
     1141 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
     1201 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
     1261 cctaacttcg atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac
     1321 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt
     1381 gcgtcgcggt gcatggagcc gggccacctc gacctaatac tagagaaaga ggagaaatac
     1441 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa
     1501 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa
     1561 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac
     1621 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc
     1681 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc
     1741 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc
     1801 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa
     1861 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt
     1921 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc
     1981 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg
     2041 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt
     2101 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgc
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