##gff-version 3
##sequence-region BBa_K315016 1 912
BBa_K315016	.	engineered_region	1	870	.	+	0	ID=BBa_S04450;Name=BBa_S04450
BBa_K315016	.	sequence_feature	837	870	.	+	0	ID=BBa_K315010;Name=BBa_K315010;Parent=BBa_S04450
BBa_K315016	.	engineered_region	1	828	.	+	0	ID=BBa_S04445;Name=BBa_S04445;Parent=BBa_S04450
BBa_K315016	.	engineered_region	105	828	.	+	0	ID=BBa_K093005;Name=BBa_K093005;Parent=BBa_S04445
BBa_K315016	.	ribosome_entry_site	105	116	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K093005
BBa_K315016	.	conserved_region	109	112	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K315016	.	CDS	123	828	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K093005
BBa_K315016	.	engineered_tag	804	828	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K315016	.	CDS	123	797	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K315016	.	engineered_region	1	96	.	+	0	ID=BBa_S04437;Name=BBa_S04437;Parent=BBa_S04445
BBa_K315016	.	sequence_feature	63	96	.	+	0	ID=BBa_J61046;Name=BBa_J61046;Parent=BBa_S04437
BBa_K315016	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S04437
BBa_K315016	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K315016	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K315016	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K315016	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K315016	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K315016	.	sequence_feature	879	912	.	+	0	ID=BBa_K315001;Name=BBa_K315001
BBa_K315016	.	sequence_feature	892	899	.	+	0	ID=annotation2070870;Name=Spacer Region;Parent=BBa_K315001
BBa_K315016	.	sequence_feature	879	891	.	+	0	ID=annotation2070869;Name=Arm (Inverted Repeat);Parent=BBa_K315001
BBa_K315016	.	sequence_feature	900	912	.	+	0	ID=annotation2070871;Name=Arm (Inverted Repeat);Parent=BBa_K315001
>BBa_K315016
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataatgt
atgctatacgaagttattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatg
cgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacg
aaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagtt
ccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttc
aaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagt
tcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttggga
agcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggt
ggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgaca
tcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccac
cggtgcttaataacgctgatagtgctagtgtagatcgctactagagataacttcgtataggatactttatacgaagtta
ttactagagataacttcgtataatgtgtactatacgaagttat