BBa_K315017
1
BBa_K315017
pTet + lox5171 Forward
2010-07-08T11:00:00Z
2015-05-08T01:11:55Z
We created this part as one step in our project.
This a promoter followed by a variant lox site.
false
false
_435_
0
7372
9
Not in stock
false
This a good construct to begin when attempting to test how variant lox sites recombine.
false
Nitya Rao
component2072781
1
BBa_K315001
component2072773
1
BBa_R0040
annotation2072781
1
BBa_K315001
range2072781
1
63
96
annotation2072773
1
BBa_R0040
range2072773
1
1
54
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986785
1
-35
range1986785
1
20
25
annotation1986787
1
-10
range1986787
1
43
48
BBa_K315001
1
BBa_K315001
variant forward lox site with 2 base changes in the spacer region (lox5171)
2010-06-14T11:00:00Z
2015-05-08T01:11:55Z
We learned about the use of variant lox sites to produce random fluorescent protein expression from the "Brainbow" paper (http://www.nature.com/nature/journal/v450/n7166/full/nature06293.html). We discovered this specific sequence in "Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination" (http://www.ncbi.nlm.nih.gov/pubmed/9714735).
The cre-lox system is used for site-specific recombination. Lox sites are 34 bp sequences of DNA that flank coding sequences. When cre recombinase is introduced, it can excise or invert the portions of DNA between two lox sites. If the two lox sites flanking the coding sequence are both forward or both reverse, the coding sequence will be excised, leaving one lox site behind. If the one site is forward and the other reverse, the coding sequence will be inverted.
false
false
_435_
0
7372
9
It's complicated
false
In constructing variant lox sites, we looked for the least promiscuous sites - those that had the lowest rates of recombination with other variants.
false
Nitya Rao
annotation2070870
1
Spacer Region
range2070870
1
14
21
annotation2070869
1
Arm (Inverted Repeat)
range2070869
1
1
13
annotation2070871
1
Arm (Inverted Repeat)
range2070871
1
22
34
BBa_K315001_sequence
1
ataacttcgtataatgtgtactatacgaagttat
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_K315017_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataatgtgtactatacgaagttat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z