BBa_K315021 1 BBa_K315021 TetA (DeOptimized from bp 144 to 290) 2010-07-13T11:00:00Z 2015-05-08T01:11:56Z This TetA gene has been deoptimized from base pairs 144 to 290 using the online Optimizer program (http://genomes.urv.es/OPTIMIZER/) and was constructed using customized Oligo segments that were annealed and ligated into a pSB1A2 vector. This sequence is the wild type TetA gene which has been deoptimized from base pairs 144 to 290. This is a basic part that can be placed behind a promoter and produce the Tetracycline resistance protein. Being that this gene is deoptimized, we are hypothesizing that it will allow for less translational efficiency as compared to the wild type TetA (no data is available at this time to support or reject this hypothesis) false false _435_ 0 7416 9 It's complicated false During the design of this sequence, it was necessary to ensure that the deoptimized sequence did not contain BioBrick restriction sites and that the translational sequence was identical to that of the wild type gene false Danielle Wagner BBa_K315021_sequence 1 atgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgccctaatgcaatttctatgtgcccccgtcctaggagccctaagtgataggtttggaaggaggcccgtcctactagccagtctactaggagccacaatagattatgccataatggccacaacacccgtcctatggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z