BBa_K315027
type: ComponentDefinition
persistent identity: BBa K315027
display id: BBa_K315027
version: 1
was generated by: iGEM to SBOL conversion
description: pTet + lox5171 forward + RBS + RFP + lox2272 forward
created: 2010-07-14T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: We created this part in an effort to determine how various lox sites interact.
owned by: james
owned by: myers
mutable description: This construct consists of a promoter followed by a "floxed" RFP gene. Because the two lox sites are not identical, we do not expect RFP to be excised when cre recombinase is introduced.
discontinued: false
dominant: false
group u list: _435_
m user id: 0
owner id: 7372
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: pTet is a moderately strong promoter. RFP is a readily visible reporter.
star: false
creator: Nitya Rao
type: DnaRegion
role: SO:0000110
role: partType/DNA
component: BBa_K315013
component: BBa_S04467
sequence annotation: BBa_S04467
sequence annotation: BBa_K315013
sequence: https://synbiohub.org/public/igem/BBa_K315027_sequence/1

BBa_K315013
type: Component
persistent identity: component2257489
display id: component2257489
version: 1
top level: BBa_K315027
definition: BBa_K315013
access: public

BBa_S04467
type: Component
persistent identity: component2257488
display id: component2257488
version: 1
top level: BBa_K315027
definition: BBa_S04467
access: public

BBa_S04467
type: SequenceAnnotation
persistent identity: annotation2257488
display id: annotation2257488
version: 1
top level: BBa_K315027
location: https://synbiohub.org/public/igem/BBa_K315027/annotation2257488/range2257488/1
component: BBa_S04467

https://synbiohub.org/public/igem/BBa_K315027/annotation2257488/range2257488/1
type: Range
persistent identity: range2257488
display id: range2257488
version: 1
top level: BBa_K315027
direction: direction/reverse
start: 1
end: 828
orientation: inline

BBa_K315013
type: SequenceAnnotation
persistent identity: annotation2257489
display id: annotation2257489
version: 1
top level: BBa_K315027
location: https://synbiohub.org/public/igem/BBa_K315027/annotation2257489/range2257489/1
component: BBa_K315013

https://synbiohub.org/public/igem/BBa_K315027/annotation2257489/range2257489/1
type: Range
persistent identity: range2257489
display id: range2257489
version: 1
top level: BBa_K315027
direction: direction/reverse
start: 837
end: 870
orientation: inline

BBa_K315013
type: ComponentDefinition
persistent identity: BBa K315013
display id: BBa_K315013
version: 1
was generated by: iGEM to SBOL conversion
description: lox2272 Forward
created: 2010-07-01T11:00:00Z
modified: 2015-05-08T01:11:55Z
mutable provenance: We learned about the use of variant lox sites to produce random fluorescent protein expression from the "Brainbow" paper (http://www.nature.com/nature/journal/v450/n7166/full/nature06293.html). We discovered this specific sequence in "Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination" (http://www.ncbi.nlm.nih.gov/pubmed/9714735
owned by: james
owned by: myers
mutable description: The cre-lox system is used for site-specific recombination. Lox sites are 34 bp sequences of DNA that flank coding sequences. When cre recombinase is introduced, it can excise or invert the portions of DNA between two lox sites. If the two lox sites flanking the coding sequence are both forward or both reverse, the coding sequence will be excised, leaving one lox site behind. If the one site is forward and the other reverse, the coding sequence will be inverted
discontinued: false
dominant: false
experience: experience/None
group u list: _435_
m user id: 0
owner id: 7371
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: In constructing variant lox sites, we looked for the least promiscuous sites - those that had the lowest rates of recombination with other variants.
star: false
creator: Jamela Peterson
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K315013_sequence/1

BBa_S04467
type: ComponentDefinition
persistent identity: BBa S04467
display id: BBa_S04467
version: 1
was generated by: iGEM to SBOL conversion
description: K315017:S04419
created: 2010-07-14T11:00:00Z
modified: 2015-05-08T01:14:40Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 7372
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Nitya Rao
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_S04419
component: BBa_K315017
sequence annotation: BBa_K315017
sequence annotation: BBa_S04419
sequence: https://synbiohub.org/public/igem/BBa_S04467_sequence/1

BBa_S04419
type: Component
persistent identity: component2250203
display id: component2250203
version: 1
top level: BBa_S04467
definition: BBa_S04419
access: public

BBa_K315017
type: Component
persistent identity: component2250197
display id: component2250197
version: 1
top level: BBa_S04467
definition: BBa_K315017
access: public

BBa_K315017
type: SequenceAnnotation
persistent identity: annotation2250197
display id: annotation2250197
version: 1
top level: BBa_S04467
location: https://synbiohub.org/public/igem/BBa_S04467/annotation2250197/range2250197/1
component: BBa_K315017

https://synbiohub.org/public/igem/BBa_S04467/annotation2250197/range2250197/1
type: Range
persistent identity: range2250197
display id: range2250197
version: 1
top level: BBa_S04467
direction: direction/reverse
start: 1
end: 96
orientation: inline

BBa_S04419
type: SequenceAnnotation
persistent identity: annotation2250203
display id: annotation2250203
version: 1
top level: BBa_S04467
location: https://synbiohub.org/public/igem/BBa_S04467/annotation2250203/range2250203/1
component: BBa_S04419

https://synbiohub.org/public/igem/BBa_S04467/annotation2250203/range2250203/1
type: Range
persistent identity: range2250203
display id: range2250203
version: 1
top level: BBa_S04467
direction: direction/reverse
start: 105
end: 828
orientation: inline

BBa_S04419
type: ComponentDefinition
persistent identity: BBa S04419
display id: BBa_S04419
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:E1010
created: 2010-01-22T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 5562
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Mary Gearing
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_E1010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_S04419_sequence/1

BBa_E1010
type: Component
persistent identity: component2244399
display id: component2244399
version: 1
top level: BBa_S04419
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component2244396
display id: component2244396
version: 1
top level: BBa_S04419
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2244396
display id: annotation2244396
version: 1
top level: BBa_S04419
location: https://synbiohub.org/public/igem/BBa_S04419/annotation2244396/range2244396/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04419/annotation2244396/range2244396/1
type: Range
persistent identity: range2244396
display id: range2244396
version: 1
top level: BBa_S04419
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2244399
display id: annotation2244399
version: 1
top level: BBa_S04419
location: https://synbiohub.org/public/igem/BBa_S04419/annotation2244399/range2244399/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_S04419/annotation2244399/range2244399/1
type: Range
persistent identity: range2244399
display id: range2244399
version: 1
top level: BBa_S04419
direction: direction/reverse
start: 19
end: 724
orientation: inline

BBa_K315017
type: ComponentDefinition
persistent identity: BBa K315017
display id: BBa_K315017
version: 1
was generated by: iGEM to SBOL conversion
description: pTet + lox5171 Forward
created: 2010-07-08T11:00:00Z
modified: 2015-05-08T01:11:55Z
mutable provenance: We created this part as one step in our project.
owned by: james
owned by: myers
mutable description: This a promoter followed by a variant lox site.
discontinued: false
dominant: false
group u list: _435_
m user id: 0
owner id: 7372
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This a good construct to begin when attempting to test how variant lox sites recombine.
star: false
creator: Nitya Rao
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0040
component: BBa_K315001
sequence annotation: BBa_R0040
sequence annotation: BBa_K315001
sequence: https://synbiohub.org/public/igem/BBa_K315017_sequence/1

BBa_R0040
type: Component
persistent identity: component2072773
display id: component2072773
version: 1
top level: BBa_K315017
definition: p(tetR)
access: public

BBa_K315001
type: Component
persistent identity: component2072781
display id: component2072781
version: 1
top level: BBa_K315017
definition: BBa_K315001
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2072773
display id: annotation2072773
version: 1
top level: BBa_K315017
location: https://synbiohub.org/public/igem/BBa_K315017/annotation2072773/range2072773/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K315017/annotation2072773/range2072773/1
type: Range
persistent identity: range2072773
display id: range2072773
version: 1
top level: BBa_K315017
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_K315001
type: SequenceAnnotation
persistent identity: annotation2072781
display id: annotation2072781
version: 1
top level: BBa_K315017
location: https://synbiohub.org/public/igem/BBa_K315017/annotation2072781/range2072781/1
component: BBa_K315001

https://synbiohub.org/public/igem/BBa_K315017/annotation2072781/range2072781/1
type: Range
persistent identity: range2072781
display id: range2072781
version: 1
top level: BBa_K315017
direction: direction/reverse
start: 63
end: 96
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K315001
type: ComponentDefinition
persistent identity: BBa K315001
display id: BBa_K315001
version: 1
was generated by: iGEM to SBOL conversion
description: variant forward lox site with 2 base changes in the spacer region (lox5171)
created: 2010-06-14T11:00:00Z
modified: 2015-05-08T01:11:55Z
mutable provenance: We learned about the use of variant lox sites to produce random fluorescent protein expression from the "Brainbow" paper (http://www.nature.com/nature/journal/v450/n7166/full/nature06293.html). We discovered this specific sequence in "Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination" (http://www.ncbi.nlm.nih.gov/pubmed/9714735).
owned by: james
owned by: myers
mutable description: The cre-lox system is used for site-specific recombination. Lox sites are 34 bp sequences of DNA that flank coding sequences. When cre recombinase is introduced, it can excise or invert the portions of DNA between two lox sites. If the two lox sites flanking the coding sequence are both forward or both reverse, the coding sequence will be excised, leaving one lox site behind. If the one site is forward and the other reverse, the coding sequence will be inverted.
discontinued: false
dominant: false
experience: experience/None
group u list: _435_
m user id: 0
owner id: 7372
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: In constructing variant lox sites, we looked for the least promiscuous sites - those that had the lowest rates of recombination with other variants.
star: false
creator: Nitya Rao
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Arm (Inverted Repeat)
sequence annotation: Spacer Region
sequence: https://synbiohub.org/public/igem/BBa_K315001_sequence/1

Arm (Inverted Repeat)
type: SequenceAnnotation
persistent identity: annotation2070869
display id: annotation2070869
version: 1
top level: BBa_K315001
location: https://synbiohub.org/public/igem/BBa_K315001/annotation2070869/range2070869/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K315001/annotation2070869/range2070869/1
type: Range
persistent identity: range2070869
display id: range2070869
version: 1
top level: BBa_K315001
direction: direction/forward
start: 1
end: 13
orientation: inline

Spacer Region
type: SequenceAnnotation
persistent identity: annotation2070870
display id: annotation2070870
version: 1
top level: BBa_K315001
location: https://synbiohub.org/public/igem/BBa_K315001/annotation2070870/range2070870/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K315001/annotation2070870/range2070870/1
type: Range
persistent identity: range2070870
display id: range2070870
version: 1
top level: BBa_K315001
direction: direction/forward
start: 14
end: 21
orientation: inline

Arm (Inverted Repeat)
type: SequenceAnnotation
persistent identity: annotation2070871
display id: annotation2070871
version: 1
top level: BBa_K315001
location: https://synbiohub.org/public/igem/BBa_K315001/annotation2070871/range2070871/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K315001/annotation2070871/range2070871/1
type: Range
persistent identity: range2070871
display id: range2070871
version: 1
top level: BBa_K315001
direction: direction/forward
start: 22
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K315027_sequence/1
type: Sequence
persistent identity: BBa K315027 sequence
display id: BBa_K315027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataatgtgtactatacgaagttattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagataacttcgtataaagtatcctatacgaagttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K315013_sequence/1
type: Sequence
persistent identity: BBa K315013 sequence
display id: BBa_K315013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttcgtataaagtatcctatacgaagttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04467_sequence/1
type: Sequence
persistent identity: BBa S04467 sequence
display id: BBa_S04467_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataatgtgtactatacgaagttattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04419_sequence/1
type: Sequence
persistent identity: BBa S04419 sequence
display id: BBa_S04419_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K315017_sequence/1
type: Sequence
persistent identity: BBa K315017 sequence
display id: BBa_K315017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataatgtgtactatacgaagttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K315001_sequence/1
type: Sequence
persistent identity: BBa K315001 sequence
display id: BBa_K315001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttcgtataatgtgtactatacgaagttat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z