LOCUS       BBa_K315028              870 bp    DNA     linear       21-Jun-2024
DEFINITION  pTet + lox5171 forward + RBS + RFP + loxBri forward
ACCESSION   BBa_K315028
VERSION     BBa_K315028.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..75
                     /label=Arm (Inverted Repeat)
     misc_feature    76..83
                     /label=Spacer Region
     misc_feature    84..96
                     /label=Arm (Inverted Repeat)
     misc_feature    63..96
                     /label=BBa_K315001
     misc_feature    1..96
                     /label=BBa_K315017
     misc_feature    109..112
                     /label=conserved
     RBS             105..116
                     /label=BBa_B0034
     CDS             123..797
                     /label=mrfp1
     misc_feature    804..828
                     /label=Help:Barcodes
     CDS             123..828
                     /label=BBa_E1010
     misc_feature    105..828
                     /label=BBa_S04419
     misc_feature    1..828
                     /label=BBa_S04467
     misc_feature    837..849
                     /label=inverted repeat arm
     misc_feature    850..857
                     /label=spacer region
     misc_feature    858..870
                     /label=inverted repeat arm
     misc_feature    851..851
                     /label=T -> A
     misc_feature    852..852
                     /label=G -> C
     misc_feature    856..856
                     /label=G -> A
     misc_feature    837..870
                     /label=BBa_K315012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agataacttc gtataatgtg tactatacga agttattact agagaaagag gagaaatact
      121 agatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag
      181 gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag
      241 gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca
      301 tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc
      361 cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg
      421 aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct
      481 acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa
      541 ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg
      601 aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca
      661 cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg
      721 acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc
      781 gtcactccac cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagataa
      841 cttcgtataa actatactat acgaagttat
//