LOCUS       BBa_K315032             3154 bp    DNA     linear       22-Nov-2024
DEFINITION  pBad + RBS + cre + pTet + loxP forward + RBS + RFP + lox5171 
            forward
ACCESSION   BBa_K315032
VERSION     BBa_K315032.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1233
                     /label=RBS-1\Strong
     RBS             1226..1230
                     /label=RBS
     misc_feature    1219..1233
                     /label=BBa_B0030
     RBS             1219..1233
                     /label=BBa_B0030
     CDS             1240..2276
                     /label=BBa_J61047
     misc_feature    1..2276
                     /label=BBa_I718008
     misc_feature    2285..2303
                     /label=TetR 1
     misc_feature    2285..2338
                     /label=BBa_R0040
     promoter        2304..2309
                     /label=-35
     misc_feature    2310..2328
                     /label=TetR 2
     promoter        2327..2332
                     /label=-10
     promoter        2285..2338
                     /label=BBa_R0040
     misc_feature    2347..2380
                     /label=BBa_J61046
     misc_feature    2285..2380
                     /label=BBa_S04437
     misc_feature    2393..2396
                     /label=conserved
     RBS             2389..2400
                     /label=BBa_B0034
     CDS             2407..3081
                     /label=mrfp1
     misc_feature    3088..3112
                     /label=Help:Barcodes
     CDS             2407..3112
                     /label=BBa_E1010
     misc_feature    2389..3112
                     /label=BBa_K093005
     misc_feature    2285..3112
                     /label=BBa_S04445
     misc_feature    3121..3133
                     /label=Arm (Inverted Repeat)
     misc_feature    3134..3141
                     /label=Spacer Region
     misc_feature    3142..3154
                     /label=Arm (Inverted Repeat)
     misc_feature    3121..3154
                     /label=BBa_K315001
     misc_feature    2285..3154
                     /label=BBa_K315030
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagat taaagaggag aaatactaga tgtccaattt actgaccgta
     1261 caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg
     1321 atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc
     1381 gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa
     1441 cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact
     1501 atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtccgggct gccacgacca
     1561 agtgacagca atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc
     1621 ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca
     1681 ctcatggaaa atagcgatcg ctgccaggat atacgtaatc tggcatttct ggggattgct
     1741 tataacaccc tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt
     1801 actgacggtg ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca
     1861 ggtgtagaga aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc
     1921 tctggtgtag ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt
     1981 gccgcgccat ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca
     2041 actcatcgat tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct
     2101 ggacacagtg cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg
     2161 gagatcatgc aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac
     2221 ctggatagtg aaacaggggc aatggtgcgc ctgctggaag atggcgatta agaatttact
     2281 agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta
     2341 ctagagataa cttcgtataa tgtatgctat acgaagttat tactagagaa agaggagaaa
     2401 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg
     2461 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac
     2521 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg
     2581 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac
     2641 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac
     2701 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc
     2761 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa
     2821 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa
     2881 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa
     2941 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa
     3001 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa
     3061 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag
     3121 ataacttcgt ataatgtgta ctatacgaag ttat
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